Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study

Eifler, Aaron C.; Lewandowski, Robert J.; Virmani, Sumeet; Chung, J.; Wang, Dingxin; Tang, Richard; Szolc-Kowalska, Barbara; Woloschak, Gayle E.; Yang, Guang; Ryu, Robert K.; Salem, Riad; Larson, Andrew C.; Cheon, Eric C.; Strouch, Matthew J.; Bentrem, David J.; Omary, Reed A.

doi:10.1016/j.jvir.2009.04.051

Cited by 16 publications

(10 citation statements)

References 33 publications

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“…Tumor cells alone are often insufficient for tumor growth, without a receptive microenvironment [34]. For example, in the rabbit VX2 model, tumors must first be grown subcutaneously, prior to transplanting the tumor into the liver or pancreas [35]. Here, we show that direct inoculation of a solid organ is possible, using gelatin sponge, which is made from collagen, an important part of the extracellular matrix in tumors.…”

Section: Plos Onementioning

confidence: 89%

Induction and characterization of pancreatic cancer in a transgenic pig model

et al. 2020

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Background Preclinical testing of new locoregional therapies for pancreatic cancer has been challenging, due to the lack of a suitable large animal model. Purpose To develop and characterize a porcine model of pancreatic cancer. Unlike small animals, pigs have similar physiology, drug dosing, and immune response to humans. Locoregional therapy in pigs can be performed using the same size catheters and devices as in humans. Methods The Oncopig is a transgenic pig with Cre-inducible TP53 R167H and KRAS G12D mutations. In 12 Oncopigs, CT-guided core biopsy of the pancreas was performed. The core biopsy was incubated with an adenoviral vector carrying the Cre recombinase gene. The transformed core biopsy was injected back into the pancreas (head, tail, or both). The resulting tumors (n = 19) were characterized on multi-phase contrast-enhanced CT, and on pathology, including immunohistochemistry. Angiographic characterization of the tumors was performed in 3 pigs. Results Pancreatic tumors developed at 19 out of 22 sites (86%) that were inoculated. Average tumor size was 3.0 cm at 1 week (range: 0.5-5.1 cm). H&E and immunohistochemical stains revealed undifferentiated carcinomas, similar to those of the pancreatobiliary system in

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Section: Plos Onementioning

confidence: 89%

Induction and characterization of pancreatic cancer in a transgenic pig model

et al. 2020

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“…While the VX2 tumor model has been used as a model of invasive pancreatic cancer before (17, 18), this complimentary study is the first to investigate clinical imaging in rabbit pancreatic cancer with the goal of PDT treatment planning. The degree to which VX2 tumors mimic the morphology and behaviour of pancreatic tumors found in humans is unknown and since the VX2 cell line is a naturally occurring rabbit squamous cell carcinoma derived from virus-induced papilloma (33), it cannot be assumed to have any structural or molecular similarity to human pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, clinical CT imaging is not compatible with the small animal models typically used in preclinical cancer research, as the spatial resolution of CT and ultrasound devices becomes a limiting factor when animal size is scaled down. Recently, a rabbit model of pancreatic cancer was described (17), and used to investigate MRI (18). We utilize this rabbit model of pancreatic cancer to complete comparative imaging and drug delivery studies.…”

Section: Introductionmentioning

confidence: 99%

Perfusion CT Estimates Photosensitizer Uptake and Biodistribution in a Rabbit Orthotopic Pancreatic Cancer Model

et al. 2015

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Objectives It was hypothesized that perfusion computed tomography (CT) blood flow (BF), blood volume (BV) and vascular permeability surface-area (PS) product parameters would be predictive of therapeutic anti-cancer agent uptake in pancreatic cancer, facilitating image-guided interpretation of human treatments. The hypothesis was tested in an orthotopic rabbit model of pancreatic cancer, by establishing the model, imaging with endoscopic ultrasound and contrast CT, and spatially comparing the perfusion maps to the ex vivo uptake values of injected photosensitizer, vertepofin. Materials and Methods Nine New Zealand White rabbits underwent direct pancreas implantation of VX2 tumors and CT perfusion or endoscopic ultrasound was performed 10 days post-implantation. Verteporfin was injected during CT imaging and tissue was removed 1 h post-injection for frozen tissue fluorescence scanning. Region-of-interest comparisons of CT data with ex vivo fluorescence and histopathological staining were performed. Results DCE-CT showed enhanced BF, BV, and PS in the tumor rim, and decreased BF, BV and PS in the tumor core. Significant correlations were found between ex vivo verteporfin concentration and each of BF, BV, and PS. Conclusions The efficacy of verteporfin delivery in tumors is estimated by perfusion CT, providing a non-invasive method of mapping photosensitizer dose.

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“…Hypervascularity, rapid growth and easy propagation in the skeletal muscle, represent its features [1][2][3][4]. It is of note that this model has been used to model various types of cancer, including those of pancreas, kidney and liver [5][6][7][8][9][10][11][12][13][14][15]. The transplantation of VX2 cells can be achieved either by implanting solid tumor pieces or by injecting the tumor cell suspensions (fresh or frozen) as previously described [16].…”

Section: Introductionmentioning

confidence: 99%