Effect of Human Cytomegalovirus and Glucose on Adhesion Molecules Expression in Cultured Human Endothelial Cells

Altannavch, Tsevegjav; Roubalová, K; Kučera, Petr; Andĕl, M

doi:10.1016/j.carpath.2004.03.278

Cited by 7 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Herein, examination of two relevant CAMs by fl ow cytometry indicated that CAM expression on astrocyte surface was enhanced after TMEV infection. Differential upregulation of CAMs has been observed in different cell types following different stimuli, including viral infection [15] . On the other hand, expression of DAF (CD55) was also enhanced in TMEV-infected astrocytes, though this enhancement was lower with the L*-1 variant than with the DA strain.…”

Section: Discussionmentioning

confidence: 99%

Differential Astrocyte Response to Theiler’s Murine Encephalomyelitis Virus Infection

Pozner¹,

Berría

Negrotto³

et al. 2005

Intervirology

View full text Add to dashboard Cite

Objectives: We aimed to address if selective astrocyte apoptosis is involved in the lack of murine demyelinating disease following infection by the L*-1 variant of Theiler’s virus. In addition, we investigated whether L*-1-infected astrocytes were able to selectively express molecules whose effects would play a role as pathogenic factors. Methods: Murine cultured astrocytes were infected with two Theiler viruses, the DA strain and the mutated DA variant L*-1, which does not synthesize the out of frame L* protein. Results: Neither DA nor L*-1 provoked apoptosis, although they replicated in astrocytes inducing GFAP and iNOS expression, as well as subsequent nitric oxide production. In addition, both viruses caused an enhanced expression of ICAM-1, VCAM-1 and decay accelerating factor (DAF). In this connection, values of VCAM-1 and DAF induced by L*-1 were higher and lower, respectively, than those induced by DA. Conclusions: Since no apoptosis was found, such mechanism would not be involved in the lack of TMEV-induced demyelinating disease by L*-1. In contrast, selective expression of VCAM-1 and DAF molecules induced by L*-1 could have a role in virus clearance from the central nervous system.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential Astrocyte Response to Theiler’s Murine Encephalomyelitis Virus Infection

Pozner¹,

Berría

Negrotto³

et al. 2005

Intervirology

View full text Add to dashboard Cite

show abstract

“…are involved in the atherosclerotic process in diabetes. 1,[9][10][11][12] SMemb was originally found in embryonic smooth muscle cells, 32) and shown to be increased at the mRNA and protein levels in diabetic meningial cells. 13) SMemb has been characterized to cause vascular smooth muscle cells to switch from contractile to synthetic type, possibly leading to atherosclerotic lesions.…”

Section: Discussionmentioning

confidence: 99%

“…Especially, expression of endothelin-1 (ET-1), a vasoconstrictive peptide, plasminogen activator inhibitor-1 (PAI-1), a prothrombic protein, and ICAM-1, VCAM-1 and ECAM-1, a group of adhesion proteins, are of significant interest in this context. [7][8][9][10][11][12] Chen et al 7,8) reported that the upregulated expression of endothelin-1 was shown in human umbilical vein endothelial cells (HUVECs) under hyperglycemic conditions, however, the behavior of PAI-1 in the cells was not studied under such conditions, hitherto.…”

mentioning

confidence: 99%

2-Amino-phenoxazine-3-one Attenuates Glucose-Induced Augmentation of Embryonic Form of Myosin Heavy Chain, Endothelin-1 and Plasminogen Activator Inhibitor-1 in Human Umbilical Vein Endothelial Cells

Fukuda

Yoshitake

Khan

et al. 2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Altannavch et al, [21] studied the effect of HCMV on expression of ICAM-1, VCAM-1, and ELAM-1 in HUVEC and described that HCMV increases expression of ICAM-1. These data are in contrast to data of the current study.…”

Section: Discussionmentioning

confidence: 99%

HCMV infection triggers smooth muscle cell proliferation in a 3D human coronary in vitro model

Voisard¹,

Göttling²,

Baur³

et al. 2013

Virol Discov

View full text Add to dashboard Cite

Background: Although a role of the human cytomegalovirus (HCMV) in atherosclerosis and restenosis is probable, clinical studies are not conclusive. The present study investigates in a threedimensional (3D) human coronary transfilter co-culture model the effect of cell-free and cell-associated HCMV-infection. Methods: Human coronary endothelial cells (HCAEC) and HCMSMC were seeded on both sides of a polycarbonate filter membrane. HCMV-infection was carried out by HCMV-infected MO. As controls MO-attack without HCMV-infection, cell free HCMV-infection, and the transfilter co-culture model without MO-attack and without HCMV-infection (Mock) were used. Results: At day 1, day 4, day 7, and day 14 the effects of HCMV-infection on MO adhesion and chemotaxis and on reactive proliferation of HCMSMC was studied. Cell-associated HCMV infection of HCAEC and HCMSMC was less intense and postponed in comparison to cell-free HCMV infection. Endothelial adhesion of MO after cellbased HCMV infection was decreased in comparison to non-infected MO, no clear effect was found on chemotaxis. Both after cell-associated and cell-free HCMV infection proliferation of HCMSMC was significantly increased. Conclusions: In the 3D human coronary transfilter co-culture model both cell-free and cell-associated HCMV-infection significantly increased proliferation of HCMSMC. If we assume that HCMV is involved in the pathogenesis of atherosclereosis and restenosis, the effect of antiviral treatments should be studied in experimental and clinical studies.

show abstract

Effect of Human Cytomegalovirus and Glucose on Adhesion Molecules Expression in Cultured Human Endothelial Cells

Cited by 7 publications

References 0 publications

Differential Astrocyte Response to Theiler’s Murine Encephalomyelitis Virus Infection

Differential Astrocyte Response to Theiler’s Murine Encephalomyelitis Virus Infection

2-Amino-phenoxazine-3-one Attenuates Glucose-Induced Augmentation of Embryonic Form of Myosin Heavy Chain, Endothelin-1 and Plasminogen Activator Inhibitor-1 in Human Umbilical Vein Endothelial Cells

HCMV infection triggers smooth muscle cell proliferation in a 3D human coronary in vitro model

Contact Info

Product

Resources

About