Effect of Hemostatics Used During Operations for Digestive Organ on Cancer Cells Present in the Peritoneal Cavity

Kubota, Nobuhiro; Kojima, Takashi; Naruse, Takayoshi

doi:10.1089/cbr.2000.15.47

Cited by 6 publications

(3 citation statements)

References 3 publications

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“…1 We reported afterward that the targets of the action of liquid form BB gelatin is mainly T cells. 6 In this study, we investigated effect of various kinds of gelatin on proliferation of murine benign and malignant cells.…”

Section: Discussionmentioning

confidence: 99%

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In VitroEffect of Gelatins on Murine Cell Proliferation

Kojima

Koide

Nagata

et al. 2001

Cancer Biotherapy and Radiopharmaceuticals

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Porcine skin (PS) gelatin suppressed proliferation of a murine hepatic cell carcinoma cell line, MH134, a murine fibrosarcoma cell line, Meth A and a murine T cell lymphoma cell line, RL Male 1. The magnitude of suppression of the proliferation by cold water fish skin (CWFS) or bovine bone (BB) gelatin was lower than that by PS gelatin. On the other hand, BB gelatin stimulated proliferation of murine spleen cells. The magnitude of stimulation of the proliferation by CWFS gelatin was lower than that by BB gelatin. PS gelatin slightly suppressed proliferation of murine spleen cells. PS gelatin induced apoptosis but not necrosis of MH134 tumor cells. CWFS gelatin induced weaker apoptosis of the cells than PS gelatin. DNA histogram indicated that PS and CWFS gelatins acted on MH134 tumor cells to increase ratios of G2 + M-phase.

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Section: Discussionmentioning

confidence: 99%

“…1 A review of the literature since 1900 failed to reveal an earlier study to report that bovine gelatin has anti-tumor activity on mice in vivo and in vitro. In this study, we investigated effect of various kinds of gelatin on proliferation of murine benign and maligant cells.…”

Section: Introductionmentioning

confidence: 99%

In VitroEffect of Gelatins on Murine Cell Proliferation

Kojima

Koide

Nagata

et al. 2001

Cancer Biotherapy and Radiopharmaceuticals

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“…Takagi et al 28 reported that brin sealant may inhibit cancer cells from detaching and spreading during surgery in mice, thus preventing the recurrence of peritonitis carcinomatosa. Kubota et al 29 carried out experiments in mice and reported that sponges can inhibit the growth of MH134 tumor cells. However, we did not nd any studies on esophageal cancer, and the effect of brin sealant on tumor growth needs further investigation.…”

Section: Cox Regression Analyses Of Overallmentioning

confidence: 99%

Long-term outcomes of the application of fibrin sealant in the prevention of cervical anastomotic leakage after McKeown esophagectomy

Huang,

Li,

et al. 2023

Preprint

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Background Anastomotic leakage remains an issue after esophagectomy. Previous studies have indicated that the intraoperative application of fibrin sealant could reduce the incidence of anastomotic leakage. This study reported survival outcomes among EC or EJC patients who received the intraoperative application of fibrin sealant during McKeown esophagectomy. Methods We designed a retrospective study including 227 patients with esophageal or esophagogastric junction cancer undergoing McKeown esophagectomy performed by our team between January 2018 and December 2019, of whom 86 patients were included in the FS group and 141 patients were included in the control group. Intraoperatively, 2.5 ml of porcine fibrin sealant was applied circumferentially to the cervical anastomosis in the FS group. The primary outcome was the impact of applying fibrin sealant to cervical anastomosis on the survival of patients after surgery. Results The median follow-up was 41 months (range, 0–52). The three-year overall survival (FS group vs. control group: 75.6% vs. 74,4%, p = 0.96) and the three-year disease-free survival (82% vs. 74,4%, p = 0.116) were not significantly different between the two groups. In the long-term follow-up, the total rates of AL between the two groups were 4.7% vs. 19.9% (p = 0.003), while the total rates of anastomotic stricture were 2.3% vs. 7.1% (p = 0.139). Cervical local recurrence was 4.7% versus 2.1% (p = 0.431). Cox regression analyses showed that the application of FS was not a predictor for overall survival. Conclusions The intraoperative application of fibrin sealant could prevent cervical anastomotic leakage after McKeown esophagectomy but did not improve survival outcomes. Further prospective clinical trials are warranted.

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Mitogenic Activity of Gelatin to Murine Spleen Cells

Nagata

Kojima

Kubota

et al. 2000

Cancer Biotherapy and Radiopharmaceuticals

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We reported previously that gelatin stimulates the growth of spleen cells in vitro. Tritium thymidine (3H-TdR) uptake into phytohemagglutinin (PHA)-stimulated spleen cells as well as intact spleen cells was augmented by gelatin. These findings suggest that gelatin serves as a mitogen for lymphoid cells. In this study, the target of action of gelatin was investigated. Tritium thymidine uptake into T cell-rich fraction was enhanced by incubation with 7.5 mg/ml of gelatin for 48 hours. The level of 3H-TdR uptake into B cell-rich fraction was not definitely increased by gelatin. Flow cytometric analysis confirmed these findings. Namely, it showed that treatment of spleen cells with 7.5 mg/ml gelatin increased a ratio of CD3-positive cells and decreased that of CD19-positive cells. Tritium thymidine uptake into natural killer cell-rich fraction was augmented by gelatin in a similar fashion to T cell-rich fraction. Tritium thymidine uptake into macrophages was very low and not affected by gelatin. Tritium thymidine uptake into macrophage-precursors was very low but was enhanced by gelatin. These findings suggest that gelatin could be used as an agent of cancer biotherapy.

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Effect of Hemostatics Used During Operations for Digestive Organ on Cancer Cells Present in the Peritoneal Cavity

Cited by 6 publications

References 3 publications

In VitroEffect of Gelatins on Murine Cell Proliferation

In VitroEffect of Gelatins on Murine Cell Proliferation

Long-term outcomes of the application of fibrin sealant in the prevention of cervical anastomotic leakage after McKeown esophagectomy

Mitogenic Activity of Gelatin to Murine Spleen Cells

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