Effect of GTP and Ca2+ on inositol 1,4,5-trisphosphate induced Ca2+ release from permeabilized rat exocrine pancreatic acinar cells

Engling, R.; Föhr, Karl J.; Kemmer, Thomas P.; Gratzl, Manfred

doi:10.1016/0143-4160(91)90079-t

Cited by 18 publications

(4 citation statements)

References 29 publications

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“…Furthermore, a physical interaction between this Ca 2+ pool and the Ins(1,4,5)P3-sensitive Ca 2+ pool was mediated by GTP. The latter observation supports previous findings in different cell types that GTP mediates the transfer of Ca 2+ from an Ins(1,4,5)P3-insensitive to an Ins(1,4,5)P3-sensitive Ca 2+ pool, thereby enlarging the Ins(1,4,5)P3-sensitive Ca 2+ pool and resulting in an enhanced Ca 2+ release following Ins(1,4,5)P3 stimulation [17][18][19][20]. The GTP evoked Ca 2+ release is probably an artifact due to the fact that the cells were permeabilized.…”

supporting

confidence: 79%

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca²⁺ from distinct intracellular pools in permeabilized lacrimal acinar cells

1995

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In permeabilized lacrimal acinar cells, cyclic ADPribose (cADP-ribose) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) release Ca 2+ in a dose dependent manner from distinct tbapsigargin-sensitive Ca 2+ pools. Ryanodine specifically blocks the Ca 2+ response to cADP-ribose, whereas heparin strongly reduces the response to Ins(1,4,5)P3 application. GTP causes a rapid Ca z+ release by a ryanodine-and heparin-insensitire mechanism and potentiates Ins(1,4,5)P 3 but not cADP-ribose evoked Ca 2+ release. It is estimated that cADP-ribose can release 16/xmol Ca2+/l cells, whereas Ins(1,4,5)P3 can mobilize 55/xmol Ca2+/l cells. The results suggest that cADP-ribose and Ins(1,4,5)P3 release Ca 2+ from distinct internal stores and that a third Ca 2+ pool exists which can selectively interact with the Ins(1,4,5)P3-sensitive Ca z+ store by a GTP-mediated process.

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supporting

confidence: 79%

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca²⁺ from distinct intracellular pools in permeabilized lacrimal acinar cells

1995

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“…In a previous report we introduced decavanadate as a specific inhibitor of the IP3 induced Ca 2 + release of rat insulinoma and rat pheochromocytoma cells [12]. In the present study we focused our interest on rat pancreatic acinar cells, which are sirnilar to permeabilized chromaffin cells [13], but in contrast 10 the above mentioned tumor cells require GTP during the repetitive IP3 induced Ca 2 + release [14]. The investigation of decavanadate effects on Ca z + release in these different cell types is of interest.…”

mentioning

confidence: 97%

Decavanadate displaces inositol 1,4,5-trisphosphate (IP3) from its receptor and inhibits IP3 induced Ca2+ release in permeabilized pancreatic acinar cells

et al. 1991

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-Inositol 1,4,5-trisphosphate (IP3) induced Ca 2 + release in digitonin permeabllized rat pancreatic acinar cells is specifically inhibited by decavanadate. The ca 2 + release induced with 0.18 flM IP3 is half maximally inhibited with approximately 5 flM decavanadate. Complete inhibition is achieved with around 20 flM decavanadate. Removal of decavanadate from the permeabilized cells fully restores sensitivity towards IP3, indicating the reversibility of the inhibition. Oligovanadate, which inhibits A TP dependent ca 2 + up1ake into intracellular stores, does not influence IP3 induced ca 2 + release. In order to reveal the mechanism underlying the effects of the different vanadate species, binding of IP3 to the same cellular preparations was investigated. We found that binding of IP3 to a high affinity receptor site (I

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“…The electrodes can also be applied for the analysis of intracellular Ca 2+ uptake and Ca 2+ release from permeabilized cells. [23][24][25][26]…”

Section: Calculation Of Ligand-metal Equilibriamentioning

confidence: 99%

[12] Calculation and control of free divalent cations in solutions used for membrane fusion studies

Föhr

Warchoł²,

Gratzl³

1993

Methods in Enzymology

112

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Section II. Conformational Changes of Proteins during Membrane Fusion 5. Protein Conformation Changes in Virus-Cell Fu-ROBERT W. DOMS sion 6. Monitoring Protein Conformational Changes dur-TETSURO YOSHIMURA ing Membrane Fusion 7. Synthetic Peptides as Probes of Function of Viral NEJAT DÜZGÜNES. 82 Envelope Proteins Section III. Membrane Fusion during Exocytosis 8. Simultaneous Electrical and Optical Measure-JOSHUA ZIMMERBERG 99 ments of Individual Membrane Fusion Events during Exocytosis 9. Visualization of Exocytosis by Quick Freezing and CARRIE J. MERKLE AND Freeze-Fracture DOUGLAS E. CHANDLER 112 10. Electropermeabilized Platelets: A Preparation to DEREK E. KNIGHT AND Study Exocytosis MICHAEL C. SCRUTTON 123 11. Exocytotic Membrane Fusion as Studied in Toxin-GUDRUN AHNERT-HILGER, Permeabilized Cells BRIGITTE STECHER, CORDIAN BEYER, AND MANFRED GRATZL 139 ν vi TABLE OF CONTENTS 12. Calculation and Control of Free Divalent Cations KARL J. FÖHR, in Solutions Used for Membrane Fusion Studies WOJCIECH WARCHOL, AND MANFRED GRATZL 13. Cytosolic Free Calcium Transients during Exocy-DANIEL P. LEW, tosis in Intact Human Neutrophils MARISA JACONI, AND TULLIO POZZAN 14. Use of Tetrahymena and Paramecium in Studies BIRGIT Η. SATIR AND of Exocytosis LEA K. BLEYM AN 174

show abstract

Effect of GTP and Ca2+ on inositol 1,4,5-trisphosphate induced Ca2+ release from permeabilized rat exocrine pancreatic acinar cells

Cited by 18 publications

References 29 publications

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca²⁺ from distinct intracellular pools in permeabilized lacrimal acinar cells

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca²⁺ from distinct intracellular pools in permeabilized lacrimal acinar cells

Decavanadate displaces inositol 1,4,5-trisphosphate (IP3) from its receptor and inhibits IP3 induced Ca2+ release in permeabilized pancreatic acinar cells

[12] Calculation and control of free divalent cations in solutions used for membrane fusion studies

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Effect of GTP and Ca2+ on inositol 1,4,5-trisphosphate induced Ca2+ release from permeabilized rat exocrine pancreatic acinar cells

Cited by 18 publications

References 29 publications

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca2+ from distinct intracellular pools in permeabilized lacrimal acinar cells

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca2+ from distinct intracellular pools in permeabilized lacrimal acinar cells

Decavanadate displaces inositol 1,4,5-trisphosphate (IP3) from its receptor and inhibits IP3 induced Ca2+ release in permeabilized pancreatic acinar cells

[12] Calculation and control of free divalent cations in solutions used for membrane fusion studies

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Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca²⁺ from distinct intracellular pools in permeabilized lacrimal acinar cells

Cyclic ADP‐ribose and inositol 1,4,5‐trisphosphate mobilizes Ca²⁺ from distinct intracellular pools in permeabilized lacrimal acinar cells