-Inositol 1,4,5-trisphosphate (IP3) induced Ca 2 + release in digitonin permeabllized rat pancreatic acinar cells is specifically inhibited by decavanadate. The ca 2 + release induced with 0.18 flM IP3 is half maximally inhibited with approximately 5 flM decavanadate. Complete inhibition is achieved with around 20 flM decavanadate. Removal of decavanadate from the permeabilized cells fully restores sensitivity towards IP3, indicating the reversibility of the inhibition. Oligovanadate, which inhibits A TP dependent ca 2 + up1ake into intracellular stores, does not influence IP3 induced ca 2 + release. In order to reveal the mechanism underlying the effects of the different vanadate species, binding of IP3 to the same cellular preparations was investigated. We found that binding of IP3 to a high affinity receptor site (I
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