Effect of glycerol-l-lactide coating polymer on bone ingrowth of bFGF-coated hydroxyapatite implants

Alt, Volker; Pfefferle, Hans-Joachim; Kreuter, Jörg; Stahl, J.P.; Pavlidis, Theodosios; Meyer, Cindy; Mockwitz, J.; Wenisch, Sabine; Schnettler, Reinhard

doi:10.1016/j.jconrel.2004.06.017

Cited by 16 publications

(14 citation statements)

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“…The limited "inductive" properties of HAP have led several investigators to develop strategies that more actively induce bone formation by delivering biologically active growth factors. For example, a series of studies have adsorbed growth factors to HAP, including fi broblast growth factor-2, [ 6 ] hepatocyte growth factor, [ 7 ] insulin-like growth factor-1, [ 8 ] osteogenin, [ 9 ] and transforming growth factor-β 1. [ 10 ] These studies have shown that adsorbed growth factors can promote bone formation by osteoblasts and osteoblast precursor cells on the surface of HAP biomaterials.…”

Section: A Modular Hydroxyapatite-binding Version Of Vascular Endothmentioning

confidence: 99%

A Modular, Hydroxyapatite‐Binding Version of Vascular Endothelial Growth Factor

2010

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Section: A Modular Hydroxyapatite-binding Version Of Vascular Endothmentioning

confidence: 99%

A Modular, Hydroxyapatite‐Binding Version of Vascular Endothelial Growth Factor

2010

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“…Stability of released FGF‐2 from coated particles showed similar levels between particles from an emulsion solution of DCM and particles from a homogenous solution of acetone of FGF‐2. Several previous studies used acetone as a solvent for a polymer coating with growth factors, and none of them reported the inactivation of the growth factors with acetone . However, the voltage of the ESP process can significantly influence the stability of growth factors.…”

Section: Discussionmentioning

confidence: 99%

Biofunctional porous anodized titanium implants for enhanced bone regeneration

Shim

Chung

Jung

et al. 2013

J Biomedical Materials Res

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Efficient osseointegration is a key factor in dental implants to reduce the total time-course of therapy. Titanium implants with anodized surface gained much interest for their enhanced osseointegration. Anodized implant combined with bioactive drugs is an ideal candidate for enhance bone regeneration. Previously delivery of drugs from the metal implants has been attempted by utilizing a polymeric dip-coating method. However, the entire surface coating with polymer can diminish the advantageous surface roughness of anodized implants and cause contact inhibition between bone and implant surface. In this study, fibroblast growth factor-2 (FGF-2) loaded poly(lactide-co-glycolide) nanoparticles were partially coated on anodized Ti discs by an electrospray deposition. Nanoparticle coated anodized discs maintained their native porous structure and provided a sustained release of FGF-2 for more than 2 weeks with 40% initial burst. In vitro study confirmed the influence of polymeric nanoparticles and the release of growth factors from the Ti disc. Nanoparticle-coated groups significantly enhanced cell spreading and differentiation. For in vivo evaluation, the anodized titanium implants were applied to rabbit tibia model. The osseointegration was estimated by bone to implant contact of best three consecutive threads at the border of the implant. The mean osteointegration value of FGF-2 releasing implant groups (70.1%) was significantly higher than that of untreated implants (47.1%). We believe that the electrospray deposition technique is a particularly attractive approach for the coating of medical devices with porous surface to maintain their surface topography while allowing a sustained delivery of growth factors for bone regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3639-3648, 2014.

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“…There is no study on the bone ingrowth of coated or uncoated HA implants limited to the cortical area of long bones as most comparable studies have studied bony incorporation of HA implants in cancellous bone of the metaphyseal‐epiphyseal region 2, 3…”

Section: Discussionmentioning

confidence: 99%

“…Implantation of the same type of uncoated HA cylinders (Endobon®) in homogenous cancellous bone in miniature pigs was shown to result in full incorporation of the implant after 80 days 2, 3. In this study, the implantation of the same HA material led to bone formation of only 27.7% of the implant area in the cortical bone area of the tibia shaft in miniature pigs suggesting important physiological differences in the incorporation of a HA bone substitute material between cancellous and cortical bone.…”

Section: Discussionmentioning

confidence: 99%

“…The coating of HA implants with growth factors to a composite material is an interesting approach for the enhancement of new bone formation combining the osteoconductive effect of the HA with other proosteogenic effects of different factors 2. Our group has used bFGF in a previous study for the coating of solid HA implants (Endobon®, Biomet Deutschland, Berlin, Germany) and could show significant enhancement of new bone formation by basic fibroblast growth factor (bFGF) coating compared with plain HA 3…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating

et al. 2008

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The combination of hydroxyapatite (HA) implants serving as osteoconductive scaffold with growth factors is an interesting approach for the improvement of bone defect healing. The purpose of this study was to test whether recombinant human bone morphogenetic protein-2 (rhBMP-2) coating of solid HA-implants improves bone formation in a cortical bone defect. Cylindrical trephine mill defects (diameter: 9.8 mm, depth: 10 mm) were created into the cortical tibia shaft of minipigs and subsequently filled either by plain HA cylinders (Endobon) or by rhBMP-2-coated HA cylinders. Fluorochrome labeling for the evaluation of time-dependent bone formation was done on days 8, 9, and 10 postsurgery with tetracyclin-100, at days 25 and 30 with alizarin-komplexon, and finally on days 32, 37, 73, and 79 with calcein green. Twelve weeks after implantation, the tibiae were harvested and were prepared for standard histological staining, fluorochrome analysis, and histomorphometry. Coating of HA implants with rhBMP-2 led to significant enhanced new bone formation of 84.7% (+/-4.6%) of the implant area with almost complete bony incorporation compared with only 27.7% (+/-8.5%) in the uncoated HA implants (p = 0.028). In both types of implants, osteoconduction of HA led to bone ingrowth of the surrounding host bone into the implants. However, only rhBMP-2-coated implants showed multitopic de novo bone formation reflecting the osteoinductive properties of rhBMP-2 in all areas of the HA implant. This study showed that the coating of HA ceramic implants with rhBMP-2 can significantly enhance new bone formation attributable to its osteoinductive effects.

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Effect of glycerol-l-lactide coating polymer on bone ingrowth of bFGF-coated hydroxyapatite implants

Cited by 16 publications

References 32 publications

A Modular, Hydroxyapatite‐Binding Version of Vascular Endothelial Growth Factor

A Modular, Hydroxyapatite‐Binding Version of Vascular Endothelial Growth Factor

Biofunctional porous anodized titanium implants for enhanced bone regeneration

Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating

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