Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull

Menzel, Viviana A.; Hinsch, Elvira; Hägele, Wolfgang; Hinsch, Klaus‐Dieter

doi:10.1111/j.1745-7262.2007.00240.x

Cited by 19 publications

(13 citation statements)

References 21 publications

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“…In agreement with these observations, it has been stated that supplementation of a lower EGCG concentration (10 μg/mL) during in vitro fertilization significantly increased fertilization rate, while higher EGCG concentration (25 μg/mL) decreased fertilization rate [ 64 ]. The same observations were reported for ARTs applied for semen/sperm, as high polyphenol levels had detrimental effects on sperm membrane integrity and mitochondrial membrane potential, sperm capacitation, sperm motility, and sperm-zona pellucid binding and thus in vitro fertilization outcomes [ 132 , 135 , 138 , 139 ]. Overall, the action of polyphenols are associated with their chemical structure, level, cell sensitivity, and half-time of reactive oxygen species, which might explain different results obtained following using polyphenolic compounds as antioxidant agents [ 132 ].…”

Section: Polyphenols and In Vitro Assisted Reproduction Techniquessupporting

confidence: 63%

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

2020

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Reproduction is a complex process that is substantially affected by environmental cues, specifically feed/diet and its components. Farm animals as herbivorous animals are exposed to a large amount of polyphenols present in their natural feeding system, in alternative feed resources (shrubs, trees, and agro-industrial byproducts), and in polyphenol-enriched additives. Such exposure has increased because of the well-known antioxidant properties of polyphenols. However, to date, the argumentation around the impacts of polyphenols on reproductive events is debatable. Accordingly, the intensive inclusion of polyphenols in the diets of breeding animals and in media for assisted reproductive techniques needs further investigation, avoiding any source of reproductive waste and achieving maximum benefits. This review illustrates recent findings connecting dietary polyphenols consumption from different sources (conventional and unconventional feeds) with the reproductive performance of farm animals, underpinned by the findings of in vitro studies in this field. This update will help in formulating proper diets, optimizing the introduction of new plant species, and feed additives for improving reproductive function, avoiding possible reproductive wastes and maximizing possible benefits.

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Section: Polyphenols and In Vitro Assisted Reproduction Techniquessupporting

confidence: 63%

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

2020

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“…2) either immediately or one hr after thawing (p > 0.05). These results are in contrast with those of Thomson et al (2009), who found that addition of genistein to the freezing media (100 µM) significantly increased total motility of human sperm, while this effect was not appreciated in bull sperm, not even after 1 hr incubation (Menzel et al, 2007). Taking in account the above data we can suggest that the effect of genistein on sperm motility differs depending on the species used.…”

Section: Py Induction In Frozen-thawed Equine Spermcontrasting

confidence: 91%

Effect of genistein addition to equine sperm freezing extender

Macías‐García

Guimarães

Lopes

et al. 2018

J Hellenic Vet Med Soc

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Cryopreservation negatively affects equine sperm quality post-thaw: an excessive release of reactive oxygen species (ROS) and increased protein tyrosine phosphorylation (known as cryocapacitation) have been demonstrated in frozenthawed equine sperm diminishing its lifespan. The objective of this study was to determine the possible beneficial effect of genistein addition (a ROS scavenger and protein tyrosine kinase inhibitor) to a commercial freezing extender. Equine sperm were frozen in the presence of different genistein concentrations ranging from 0 to 800 μM. After thawing, the sperm viability (eosinnigrosin), acrosome integrity using peanut agglutinin (PNA), protein tyrosine phosphorylation (PY) and motility were assessed at times 0 and 1 hr. In addition PY was studied in fresh and frozen sperm incubated in Modified Whitten’s (MW) medium with 25 mM Bicarbonate (MW+Bic). Genistein did not affect the viability, total or progressive motility or acrosome status of frozenthawed equine sperm. Immediately after thawing, positive PY staining in the equatorial band was observed and genistein addition did not exert any change in PY. Fresh sperm incubated in MW+Bic showed a significant increase in PY staining along the tail compared to frozen-thawed sperm. In our study sperm viability immediately post-thaw was 58.25% ± 5.35 and progressive motility 14.46% ± 5.7 (mean ± S.E.M.) and genistein did not improve the post-thaw quality of equine sperm. In addition, cryopreservation did not induce PY immediately post-thaw or enhanced frozen-thawed equine sperm susceptibility to PY induction.

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“…Besides these differences related to concentration, another question to take into consideration is related to the possible different mechanisms for induced npg capacitation of fresh spermatozoa and cryocapacitation, as previously described in bull spermatozoa [53]. In the frozen bull spermatozoa, the genistein did not affect tyrosine phosphorylation [54], suggesting that cryopreservation affects the regulatory mechanism of capacitation [53,54].…”

Section: Resultsmentioning

confidence: 94%

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 mmol L -1 ) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozenthawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

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Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull

Abstract: Our results suggest that in cryopreserved bovine spermatozoa, genistein affects a protein tyrosine phosphorylation-independent signal transduction pathway that is involved in sperm capacitation, the acrosome reaction and sperm-zona pellucida binding.

Cited by 19 publications

References 21 publications

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

Effect of genistein addition to equine sperm freezing extender

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

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