Effect of genistein addition to equine sperm freezing extender

Macías‐García, Beatriz; Guimarães, Tatiana; Lopes, G.; Rocha, A.; González‐Fernández, Lauro

doi:10.12681/jhvms.15868

Cited by 8 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of genistein upon equine semen quality has also been observed, with the addition of genistein to equine sperm (up to 800 µM) not significantly influencing viability [ 86 ]. Genistein also did not affect sperm motility or acrosomal status immediately and up to one hour after thawing [ 86 ]. It can be concluded that the beneficial effect of genistein on sperm motility is species-specific and may also be breed-specific.…”

Section: Effects On Male Reproductive Physiologymentioning

confidence: 99%

Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock

Wyse

Latif

Gurusinghe

et al. 2022

Animals

View full text Add to dashboard Cite

Legume crops and pastures have a high economic value in Australia. However, legume species commonly used for grazing enterprises have been identified to produce high concentrations of phytoestrogens. These compounds are heterocyclic phenolic, and are similar in structure to the mammalian estrogen, 17β-estradiol. The biological activity of the various phytoestrogen types; isoflavones, lignans and coumestans, are species-specific, although at concentrations of 25 mg/kg of dry matter each of the phytoestrogen types affect reproductive functions in grazing livestock. The impacts upon fertility in grazing livestock such as cattle and sheep, vary greatly over length of exposure time, age and health of animal and the stress stimuli the plant is exposed to. More recently, research into the other effects that phytoestrogens may have upon metabolism, immune capacity and growth and performance of grazing livestock has been conducted. Potential new benefits for using these phytoestrogens, such as daidzein and genistein, have been identified by observing the stimulation of production in lymphocytes and other antibody cells. Numerous isoflavones have also been recognized to promote protein synthesis, increase the lean meat ratio, and increase weight gain in cattle and sheep. In Australia, the high economic value of legumes as pasture crops in sheep and cattle production enterprises requires proactive management strategies to mitigate risk associated with potential loss of fertility associated with inclusion of pasture legumes as forages for grazing livestock.

show abstract

Section: Effects On Male Reproductive Physiologymentioning

confidence: 99%

Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock

Wyse

Latif

Gurusinghe

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…Martinez-Soto et al (2010) genistein is an isoflavone compound that has a direct effect on the function of mature spermatozoa by modifying the capacitation process and acrosome reaction. Genistein modifies membrane hemodialysis and causes a significant reduction in ROS (Garcia et al, 2015). On the other hand, genistein has a protective antioxidant effect on sperm DNA integrity.…”

Section: Discussionmentioning

confidence: 99%

Effect of Genistein Supplementation in Tris-Aminomethane Base Extender on Characteristics of Pre-Freezing and Post-Thawed Sperm Ongole Grade Bull

Nugraha¹,

Putri²,

Ardiantoro³

et al. 2023

AAVS

View full text Add to dashboard Cite

Sperm undergo physiological changes during the cooling and freezing process, and especially significant changes are seen after freezing than after cooling. This research analyzed the effect of genistein supplementation in the basic diluent of tris-aminomethane on the quality of spermatozoa. Bulls aged 3-4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4-5 0 C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane-egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effect of the antioxidant genistein on tris aminomethane -egg yolk diluent gave a significant effect on sperm viability in the Before Freezing (BF) phase at 1.5 hours, BF and PT at two hours (P<0.05). The addition of different genistein in diluent tris aminomethane -egg yolk was no significant effect (P>0.05) on sperm abnormality in both the BF and PT phases at 1 hours, 1.5 hours and 2 hours. The addition of genistein with a concentration of 30 µM was able to maintain better semen quality.

show abstract

“…Kumi-Diaka et al [ 233 ] stated that low doses of GEN (30 μg/mL) did not affect mice sperm motility, however its high concentrations (70 and 100 μg/mL) interfered with the sperm motion behavior. According to Macías-García et al [ 234 ], none of the GEN doses tested (0–800 μmol/L) had any effect in frozen-thawed equine sperm motility or viability. The fact that the addition of GEN did not significantly improve the basic quality parameters of semen led to the assumption that excessive ROS production prior, or during freezing may not be the main factor affecting equine sperm vitality exposed to low temperatures in comparison to cold or osmotic shock.…”

Section: Genisteinmentioning

confidence: 99%

“…Furthermore, besides differences related to an adequate GEN concentration, another question that needs to be considered, is related to the possibility that different mechanisms are responsible for induced capacitation of fresh or cryopreserved spermatozoa. Indeed, in case of frozen bull or equine spermatozoa, the biomolecule did not affect tyrosine phosphorylation or the acrosome status [ 234 , 240 , 241 ], suggesting that the process of cryopreservation per se affects the regulatory mechanisms of capacitation.…”

Section: Genisteinmentioning

confidence: 99%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

View full text Add to dashboard Cite

Emerging evidence from in vivo as well as in vitro studies indicates that natural biomolecules may play important roles in the prevention or management of a wide array of chronic diseases. Furthermore, the use of natural compounds in the treatment of male sub- or infertility has been proposed as a potential alternative to conventional therapeutic options. As such, we aimed to evaluate the effects of selected natural biomolecules on the sperm production, structural integrity, and functional activity. At the same time, we reviewed their possible beneficial or adverse effects on male reproductive health. Using relevant keywords, a literature search was performed to collect currently available information regarding molecular mechanisms by which selected natural biomolecules exhibit their biological effects in the context of male reproductive dysfunction. Evidence gathered from clinical trials, in vitro experiments and in vivo studies suggest that the selected natural compounds affect key targets related to sperm mitochondrial metabolism and motion behavior, oxidative stress, inflammation, DNA integrity and cell death. The majority of reports emphasize on ameliorative, stimulating and protective effects of natural biomolecules on the sperm function. Nevertheless, possible adverse and toxic behavior of natural compounds has been indicated as well, pointing out to a possible dose-dependent impact of natural biomolecules on the sperm survival and functionality. As such, further research leading to a deeper understanding of the beneficial or adverse roles of natural compounds is necessary before these can be employed for the management of male reproductive dysfunction.

show abstract

Effect of genistein addition to equine sperm freezing extender

Cited by 8 publications

References 24 publications

Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock

Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock

Effect of Genistein Supplementation in Tris-Aminomethane Base Extender on Characteristics of Pre-Freezing and Post-Thawed Sperm Ongole Grade Bull

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

Contact Info

Product

Resources

About