Effect of Genistein and Daidzein on the Proliferation and Differentiation of Human Preadipocyte Cell Line

Abstract

Isoflavones are known to have several biological activities, including a hypolipidemic effect. However, the mechanism of the lipid lowering effect of genistein remains to be elucidated. There is conflicting evidence on the effect of genistein for the deposition of adipocyte tissues. We examined the effect of the isoflavones on the growth and differentiation of human preadipocyte cells, AML-I. Growth arrest accompanied by the appearance of characteristics of apoptosis was observed by genistein or daidzein treatment under the adipogenic stimulation. The expressions of apoptosis-related proteins, Bad, Akt, and p-Akt, were modulated in the genistein-treated cells by Western blot analysis. On the other hand, exposure of AML-I to the isoflavones increased accumulation of cytoplasmic lipid droplets. Actually, the cytoplasmic expressions of fatty acid synthase (FAS) and peroxisome proliferator-activated receptor (PPAR)-gamma were increased in the genistein-treated cells. Glycosylated forms of the isoflavones genistein and puerarin did not have such activities. These results suggested that only aglycon forms of isoflavones induced not only apoptosis but also lipogenesis in the preadipocyte cell line AML-I. The possible mechanism of these phenomena has been discussed in the text.

RNA‐Seq analysis reveals the potential molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of finishing Xianan beef cattle

“…Some studies showed that daidzein could promote adipogenic differentiation and fat deposition (Cho et al, 2010;Hirota et al, 2010;Li et al, 2011). Some studies showed that daidzein could promote adipogenic differentiation and fat deposition (Cho et al, 2010;Hirota et al, 2010;Li et al, 2011).…”

Daidzein enhances intramuscular fat deposition and improves meat quality in finishing steers

“…Daidzein and equol enhanced adipocyte differentiation, formation of lipid droplets, and lipid accumulation in murine and human preadipocytes. 7,8,34 Rehfeldt et al 9 investigate the effects of dietary daidzein during late gestation on neonatal body composition and found that daidzein supplementation did not affect subcutaneous fat content but significantly elevated the concentration of fat in the whole piglet body. Similar to our results, Crespillo et al 33 observed that supplemental daidzein tended to increase the fat content in skeletal muscle and reduced that in liver of male Wistar rats.…”

Anti-atherosclerotic action of Ger-Gen-Chyn-Lian-Tang and AMPK-dependent lipid lowering effect in hepatocytes

“…In animal models, puerarin also can attenuate high glucose-and diabetes-induced VSMCs proliferation . Moreover, aglycon form of puerarin could cause lipogenesis via increasing fatty acid synthase (FAS) and peroxisome proliferator-activated receptor g (PPARg) in a preadipocyte cell line (Hirota et al, 2010). In addition, wogonin was suggested to modulate atherosclerotic change in vitro (Sieveking et al, 2005), and baicalein was shown to inhibit VSMC proliferation, and suppress intimal hyperplasia after balloon vascular injury in the rat (Peng et al, 2008).…”