Effect of Fungal Natural Products in an Agrobacterium tumefaciens Potato Disc Assay

Abstract

An Agrobacterium tumefaciens potato disc assay was used to screen certain natural products (25 from fungi for crown gall tumor/antitumor induction. Monorden (-75.0%), cladosporin (-79-0%), monocillin IV ( -79.6%), duclauxin ( -96.0%), diplodiol (-96.3%), and chaetoglobosin K (-99.0%) displayed concentration-dependent responses at 5, 10, 25, and 50 µg/disc. These natural products were not antimicrobial as determined by sensitivity tests using fungi and bacteria, inclusive of A. tumefaciens.

Isolation, structural elucidation of flavonoid constituents fromLeptadenia pyrotechnicaand evaluation of their toxicity and antitumor activity

“…The potato disc bioassays for the plant extracts were carried out as described elsewhere (Ferrigni et al, 1982;Ferrigni & McLaughlin, 1984;Anderson et al, 1988Anderson et al, , 1992Bryant et al, 1994). Tumors were initiated on potato discs (usually Pontiac red or red Russet variations).…”

“…Crown gall tumorigenesis on discs of potato tuber Solanum tuberosum L. Solanaceae was proposed as an ideal system for investigating the transformation process (Ferrigni & McLaughlin, 1984). The action of the antitumor compounds is neither via antibiosis nor through inhibition of bacterial attachment to the tumor-binding sites (Bryant et al, 1994).…”

Structural elucidation and evaluation of toxicity and antitumor activity of cardiac glycosides isolated fromLeptadenia pyrotechnica

“…The potato disk bioassay for the plant extracts was carried out in accordance to (Ferrigni & McLughlin, 1984;Ferrigni et al, 1982;Anderson et al, 1988Anderson et al, , 1992Bryant et al, 1994). Tumors were initiated on potato disks (usually Pontiac red or red russet variations).…”

“…The action of the antitumor compounds is neither via antibiosis nor through inhibition of bacterial attachment to the tumor-binding sites (Bryant et al, 1994). This paper deals with the isolation, structural elucidation of cardiac glycosides in L. pyrotechnica and evaluation of their antitumor activity and toxicity using potato disk assay and brine shrimp, respectively.…”

GC-MS investigation and toxicological evaluation of alkaloids fromLeptadenia pyrotechnica

“…The potato disc bioassay for the plant extracts were carried out as described elsewhere Ferrigni & McLughlin, 1984;Anderson et al, 1988Anderson et al, , 1992Bryant et al, 1994). Tumors were initiated on potato discs (usually Pontiac red or red russet variations).…”