GC-MS investigation and toxicological evaluation of alkaloids fromLeptadenia pyrotechnica

Abstract

GC-MS analysis of isolated alkaloids from aerial parts of Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceae) was performed. Twenty-four alkaloids and six simple amines were detected for the first time in this plant. Almost all of the alkaloids belonged to pyridine, pyrrole, pyrazine, and indole types. The acute LC 50 of the total alkaloids and alcohol extracts estimated by means of brine shrimp toxicity test were 63.09 and 11.89 ppm, respectively. The antitumor activities of these extracts, using potato disc screen, showed good activity represented by −33.6% and −49.3%, respectively.

Antioxidant, carbonic anhydrase inhibition and diuretic activity of Leptadenia pyrotechnica Forssk. Decne

“…Among glycosides it contained both cardiac and pregnane glycosides [ 10 ]. The GC-MS analysis of alkaloid rich extract of L. pyrotechnica indicated the presence of pyridine alkaloids while other alkaloids were also present in minor quantities [ 11 ]. Among phenolics and flavonoids it contained caffeic acid, gallic acid, vanillic acid, epicatechin, kaempferol-, quercetin-, texacin-flavonoids and cardenolides [ [12] , [13] , [14] ].…”