Effect of fungal elicitation on indirubin production from a suspension culture of Polygonum tinctorium

Marero, Lydia M.; Jin, Je Hyeong; Shin, Joong Han; Lee, Ho Jae; Chung, In Sik; Lee, Hyong Joo

doi:10.1016/s0141-0229(96)00230-x

Cited by 16 publications

(1 citation statement)

References 11 publications

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“…Nowadays, employment of fungal preparations as elicitors has become one of the most important and successful measures to enhance secondary metabolite production in plant cell cultures. Representative examples included Silybum marianum cell cultures for silymarin production [17], Polygonum tinctorium cell cultures for indirubin production [18], Salvia miltiorrhiza cell cultures for tanshinone production [19], and Dioscorea galesttiana cell cultures for diosgenin production [20]. Fungal elicitors were utilized mainly in the form of living or autoclaved mycelia, crude extracts, peptides, proteins and saccharides [12,13].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17

Mao

Lou

et al. 2011

Molecules

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The effects of the oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 as elicitors on diosgenin production in cell suspension cultures of its host Dioscorea zingiberensis were investigated. Three oligosaccharides, DP4, DP7 and DP10, were purified from the oligosaccharide fractions DP2-5, DP5-8 and DP8-12, respectively, which were prepared from the water-extracted mycelial polysaccharide of the endophytic fungus F. oxysporum Dzf17. When the cell cultures were treated with fraction DP5-8 at 20 mg/L on day 26 and harvested on day 32, the maximum diosgenin yield (2.187 mg/L) was achieved, which was 5.65-fold of control (0.387 mg/L). When oligosaccharides DP4, DP7 and DP10 were individually added to 26-day-old D. zingiberensis cell cultures at concentrations of 2, 4, 6, 8 and 10 mg/L in medium, DP7 at 6 mg/L was found to significantly enhance diosgenin production, with a yield of 3.202 mg/L, which was 8.27-fold of control. When the cell cultures were treated with DP7 twice on days 24 and 26, and harvested on day 30, both diosgenin content and yield were significantly increased and reached the maximums of 1.159 mg/g dw and 4.843 mg/L, both of which were higher than those of single elicitation, and were 9.19- and 12.38-fold of control, respectively.

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