Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17

Abstract

The effects of the oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 as elicitors on diosgenin production in cell suspension cultures of its host Dioscorea zingiberensis were investigated. Three oligosaccharides, DP4, DP7 and DP10, were purified from the oligosaccharide fractions DP2-5, DP5-8 and DP8-12, respectively, which were prepared from the water-extracted mycelial polysaccharide of the endophytic fungus F. oxysporum Dzf17. When the cell cultures were treated with fraction DP5-8 at 20 mg/L on day 26 and harvested on day 32, the maximum diosgenin yield (2.187 mg/L) was achieved, which was 5.65-fold of control (0.387 mg/L). When oligosaccharides DP4, DP7 and DP10 were individually added to 26-day-old D. zingiberensis cell cultures at concentrations of 2, 4, 6, 8 and 10 mg/L in medium, DP7 at 6 mg/L was found to significantly enhance diosgenin production, with a yield of 3.202 mg/L, which was 8.27-fold of control. When the cell cultures were treated with DP7 twice on days 24 and 26, and harvested on day 30, both diosgenin content and yield were significantly increased and reached the maximums of 1.159 mg/g dw and 4.843 mg/L, both of which were higher than those of single elicitation, and were 9.19- and 12.38-fold of control, respectively.

Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity

“…The major phytoconstituents present in the root extract of H. isora are steroidal sapogenins belonging to triterpenes group (Diosgenin, neolignans and rosmarinic) (Li et al 2011;Patel et al 2012;Kumar et al 2007). The possible mechanism for the reduction of Ag ?…”

Effect of endophytic Bacillus sp. from selected medicinal plants on growth promotion and diosgenin production in Trigonella foenum-graecum

“…Synthesis of secondary metabolites like saponins has been suggested as an adaptive strategy in plants to respond to environmental stress. The studies of Walker et al (2011) clearly demonstrates the role of The effects of oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 to elicit the biosynthesis of diosgenin in cell suspension cultures of Dioscorea zingiberensis has also been previously reported (Li et al 2011).…”

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

“…Cultures treated with chitosan contained 72 proteins when compared to the untreated controls whereas 27 proteins found in controls were not detected in chitosan-treated Moss Plants (Physcomitrella patens) cultures. Exopolysaccharide (EPS) and water-extracted mycelial polysaccharide (WOS) from the endophytic fungus Fusarium oxysporumDzf17 significantly increased the activities of phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD), suggesting that the oligosaccharides from the endophytic fungus F. oxysporum Dzf17 may be related to the activation and enhancement of the defensive mechanisms of D. zingiberensis suspension cell and seedling cultures (Li et al 2011).After methyl jasmonate (MeJA) treatment, which is considered an elicitor of secondary metabolites, 388 candidate MeJA-responsive unique transcripts were identified in Sweet Basil (Ocimum basilicum). Transcript analysis suggests that in addition to controlling its own biosynthesis and stress responses, MeJA up-regulates transcripts of the various secondary metabolic pathways, including terpenoids and phenylpropanoids flavonoids.…”

“…An inducer can promote a lot of ingredients in one plant, but the effect on various secondary metabolites is different (Table 1) (Akcapinar et al 2015) (Akcapinar et al 2015).Different elicitors derived from one fungal have different effects on promoting secondary metabolites in medical plants (Li et al 2011), so it's necessary to find out the most effective elicitor. However, addition of fungal elicitor for significantly stimulating secondary metabolites accumulation in the cell culture of Calendula officinalis and its secretion into the culture medium, the elicitors also caused slight inhibition of Calendula officinalis cell growth (Wiktorowska et al 2010).…”