Effect of endophytic Bacillus sp. from selected medicinal plants on growth promotion and diosgenin production in Trigonella foenum-graecum

Abstract

Endophytic microorganisms support the growth of host plants by production of phytohormones, N 2 fixation, synthesis of ACC deaminase, phosphate solubilization and also by means of production of antimicrobial metabolites or siderophores. In the present study, effect of endophytic Bacillus sp. was analysed on the growth enhancement and metabolite modulation in the seedlings of Fenugreek (Trigonella foenum-graecum L.), a well known spice with medicinal properties. Preliminary screening has resulted in the identification of strains CaB 1 (Bacillus cereus), CaB 2 (Bacillus thuringiensis) and CaB 5 (Bacillus sp.) isolated from Capsicum annuum and EcB 4 (Bacillus sp.) isolated from Elettaria cardamomum to have excellent effect on seedling growth enhancement in Fenugreek. The plantlets formed from the treatment were further analysed for leaf number, shoot length, root length, wet weight and diosgenin concentration, which is the principal secondary metabolite of the plant. Interestingly, HPLC analysis has confirmed the ability of CaB 5 to induce highest level of diosgenin biosynthesis (527.83 ± 13.5 lg/mL). The obtained results are of great significance as it widens the exploration of endophytic bacteria on enhanced synthesis of medicinal natural products.

Identification of a novel endophytic Bacillus sp. from Capsicum annuum with highly efficient and broad spectrum plant probiotic effect

“…The results obtained in the study is also in agreement with our previous report using the same CaB 5 where it was found to have diosgenin enhancement effect in Trigonella foenum‐graecum and hardening period reduction in tissue cultured banana ( Musa accumilata ) (Jasim et al . ; Suada et al . ).…”

“…In our previous studies also, the same CaB 5 was found to have tremendous modulatory effect on diosgenin production in Trigonella foenum‐graecum (Fenugreek) and was also found to reduce the hardening period of tissue‐cultured Musa acuminata (Jasim et al . ; Suada et al . ).…”

Identification and functional characterization of DzS3GT, a cytoplasmic glycosyltransferase catalyzing biosynthesis of diosgenin 3-O-glucoside in Dioscorea zingiberensis

“…and Trillium, produce pharmaceutical diosgenin, which usually exists in plants in the form of saponins and has been a starting material for the production of steroids over seven decades (Ding et al 1983;Jasim et al 2015;Marker et al 1940). Steroidal saponins vary in structure because of their various patterns of steroidal oxygenation and glycosylation (Challinor et al 2013;Wang and Hou 2008), which may be associated with a broad range of biological activities, such as cytotoxic (Wen et al 2015), antiulcer (Júnior et al 2015), hypolipidemic, anti-inflammatory, antioxidant, and antifungal effects (Sparg et al 2004;Weng et al 2014).…”

Relative efficiency of positive selection and tissue culture for generating pathogen-free planting materials of yam (Dioscorea spp.)

“…Previous studies on contamination control in yam tissue cultures advocated the use of a combination of five antibiotics, with 33% success and this was specific to some genotypes (Mbah & Wakil 2012). However, endophytes may not be completely undesirable as various endophytic bacteria in association with plants were reported to increase the adaptive response of plants to stress through plant growth stimulation (nitrogen fixation, auxin and cytokinin production) and disease protective properties (Jasim et al 2015). In black pepper, the endophyte Pseudomonas fluorescens increased production of defence enzymes against the pathogen Phytophthora capsici (Paul & Sarma 2005).…”