Innate endophytic fungus, Aspergillus terreus as biotic elicitor of withanolide A in root cell suspension cultures of Withania somnifera

Kushwaha, Ramesh Kumar; Singh, Satnam; Pandey, Shiv Shanker; Kalra, Alok; Babu, Chikkarasanahalli Shivegowda Vivek

doi:10.1007/s11033-019-04641-w

Cited by 15 publications

(3 citation statements)

References 61 publications

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“…Licorice endophyte fungus, A. eucalypticola SBU-11AE (stored on the sand at 4 °C) that produce glabridin (data not published), obtained from Beneficial Microorganisms Lab, Department of Agriculture, Medicinal Plant and Drugs Research Institute (MPDRI), Shahid Beheshti University, Tehran, Iran. To prepare the working culture of fungus, 100–200 mg of inoculated sand was cultured on 8 cm Petri dish containing PDA (Potato Dextrose Agar) medium and incubated at 28 °C for seven days ( Figure 2(C) ) [ 34 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae)

Bahadori Ganjabadi,

Farzaneh,

Mirjalili

2023

Mycobiology

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Glabridin is a well-known active isoflavone found in the root of licorice ( Glycyrrhiza glabra L.) that possess a wide range of biological activity. Plant cells, hairy roots, and fungal endophytes cultures are the most important alternative methods for plant resources conservation and sustainable production of natural compounds, which has received much attention in recent decades. In the present study, an efficient culture condition was optimized for the biomass accumulation and glabridin production from fungal endophyte Aspergillus eucalypticola SBU-11AE isolated from licorice root. Type of culture medium, range of pH, and licorice root extract (as an elicitor) were tested. The results showed that the highest and lowest biomass production was observed on PCB medium (6.43 ± 0.32 g/l) and peptone malt (5.85 + 0.11 g/l), respectively. The medium culture PCB was produced the highest level of glabridin (7.26 ± 0.44 mg/l), while the lowest level (4.47 ± 0.02 mg/l) was obtained from the medium peptone malt. The highest biomass (8.51 ± 0.43 g/l) and glabridin (8.30 ± 0.51 mg/l) production were observed from the PCB medium adjusted with pH = 6, while the lowest value of both traits was obtained from the same medium with pH = 7. The highest production of total glabridin (10.85 ± 0.84 mg/l) was also obtained from the culture medium treated with 100 mg/l of the plant root extract. This information can be interestingly used for the commercialization of glabridin production for further industrial applications.

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Section: Methodsmentioning

confidence: 99%

Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae)

Bahadori Ganjabadi,

Farzaneh,

Mirjalili

2023

Mycobiology

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“…Recently, different studies have demonstrated the role of endophytes in the primary and secondary metabolism of plants, like the identification of endophytes promoting plant growth, and enhancing in-planta vindoline, ajmalicine, and serpentine biosynthesis in Catharanthus roseus (Tiwari et al 2013 ; Pandey et al 2016b ; Singh et al 2020 ). The role of endophytes in enhancing the withanolide A (WLA) and withaferin A (WFA) biosynthesis in Withania somnifera has also been reported (Pandey et al 2018 ; Kushwaha et al 2019a , b , c ). Moreover, the WFA, which is usually found in leaves, was detected in the roots of the endophyte-treated plants, thus suggesting the role of endophytes in modulating the site of WFA biosynthesis in W. somnifera plant (Pandey et al 2018 ).…”

Section: Introductionmentioning

confidence: 94%

“…Recently, the compatibility of Trichoderma viride with the fungal endophytes of W. somnifera was also studied, and it was observed that the co-inoculation of T. viride along with inherent endophytes of W. somnifera enhanced the plant growth and in-planta withanolide accumulation (Kushwaha et al 2019c ). An endophytic fungus ( Aspergillus terreus ) of W. somnifera has been used as a biotic elicitor for increasing the biosynthesis of WLA in the root cell suspension culture of W. somnifera (Kushwaha et al 2019b ). Therefore, the endophytic community associated with a particular host plant has special importance as a source of potential microbes that may be used to promote plant growth, protect plants from abiotic and biotic stresses, and increase biosynthesis of secondary metabolites of host plants, and as an in-vitro platform for the production of host-similar-secondary metabolites or other therapeutic compounds.…”

Section: Introductionmentioning

confidence: 99%

Unraveling endophytic diversity in dioecious Siraitia grosvenorii: implications for mogroside production

Tamang,

Kaur,

Thakur

et al. 2024

Appl Microbiol Biotechnol

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Host and tissue-specificity of endophytes are important attributes that limit the endophyte application on multiple crops. Therefore, understanding the endophytic composition of the targeted crop is essential, especially for the dioecious plants where the male and female plants are different. Here, efforts were made to understand the endophytic bacterial composition of the dioecious Siraitia grosvenorii plant using 16S rRNA amplicon sequencing. The present study revealed the association of distinct endophytic bacterial communities with different parts of male and female plants. Roots of male and female plants had a higher bacterial diversity than other parts of plants, and the roots of male plants had more bacterial diversity than the roots of female plants. Endophytes belonging to the phylum Proteobacteria were abundant in all parts of male and female plants except male stems and fruit pulp, where the Firmicutes were most abundant. Class Gammaproteobacteria predominated in both male and female plants, with the genus Acinetobacter as the most dominant and part of the core microbiome of the plant (present in all parts of both, male and female plants). The presence of distinct taxa specific to male and female plants was also identified. Macrococcus, Facklamia, and Propionibacterium were the distinct genera found only in fruit pulp, the edible part of S. grosvenorii. Predictive functional analysis revealed the abundance of enzymes of secondary metabolite (especially mogroside) biosynthesis in the associated endophytic community with predominance in roots. The present study revealed bacterial endophytic communities of male and female S. grosvenorii plants that can be further explored for monk fruit cultivation, mogroside production, and early-stage identification of male and female plants. Key points • Male and female Siraitia grosvenorii plants had distinct endophytic communities • The diversity of endophytic communities was specific to different parts of plants • S. grosvenorii-associated endophytes may be valuable for mogroside biosynthesis and monk fruit cultivation

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Insights into Role of Invisible Partners in Plant Growth and Development

Bharadwaj

Kumar

Ramalingam

2021

Plant in Challenging Environments

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Innate endophytic fungus, Aspergillus terreus as biotic elicitor of withanolide A in root cell suspension cultures of Withania somnifera

Cited by 15 publications

References 61 publications

Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae)

Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae)

Unraveling endophytic diversity in dioecious Siraitia grosvenorii: implications for mogroside production

Insights into Role of Invisible Partners in Plant Growth and Development

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