Effect of freezing–thawing process and quercetin on human sperm survival and DNA integrity

Zribi, Nassira; Chakroun, N.; Abdallah, Ferjani Ben; Elleuch, H.; Sellami, Afifa; Gargouri, Jalel; Rebaï, Tarek; Fakhfakh, Faiza; Keskes, Leila

doi:10.1016/j.cryobiol.2012.09.003

Cited by 113 publications

(89 citation statements)

References 29 publications

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“…Supplementation of the cryopreservation medium with 50 mM querctin resulted in a significant improvement in post-thaw motility, viability, and DNA integrity [Zribi et al 2012]. The addition of catalase resulted in a significant increase in the percent of progressive motility and viability, along with a significant decrease in DNA damage [Moubasher et al 2013].…”

Section: Utility Of Cryoadditivesmentioning

confidence: 86%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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Sperm cryopreservation preserves the fertility of cancer patients undergoing chemotherapy, ensures sperm are available at the time of oocyte retrieval in assisted reproductive technology (ART) procedures and avoids the need for repeated sperm extraction surgeries in azoospermic patients. Conventional methods of cryopreservation involve storage in liquid nitrogen (LN 2 ), which causes a significant decline in sperm parameters such as motility and viability and results in DNA damage. Newer methods of sperm cryopreservation such as the LN 2 vapor method, vitrification, and experimental methods such as lyophilization, have significant advantages over the conventional methods in terms of cost effectiveness and ease of use. Density gradient centrifugation (DGC), swim up, and magnetic assisted cell sorting (MACS) can be used prior to or post-cryopreservation to improve post-thaw sperm quality. Cryopreservation in special carriers such as cryoloops and empty zona prevents the loss of small numbers of sperm during cryopreservation. This article will discuss these sperm preservation and selection techniques.

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Section: Utility Of Cryoadditivesmentioning

confidence: 86%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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“…Of all the known caspases, caspase-3 plays an important role downstream of the apoptotic cascade (Fuentes-Prior and Salvesen, 2004), and its activation has been reported in the cryopreserved sperm of several mammalian species Zribi et al, 2012). Therefore, we examined whether treatment of sperm with MBCD activates caspase-3 using 2 different methodologies.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken

et al. 2016

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Cryopreserved sperm undergoes serious damage which affects its fertilizing ability. Despite progress in understanding the nature of functional deterioration in mammalian sperm, little is known about the mechanism involved in the induction of functional damage in avian sperm. Cellular membranes are considered the primary site of cryodamage to sperm. Membrane rafts are specific membrane regions enriched in sterols, ganglioside G M1 , and functional proteins and they play important roles in the regulation of diverse functions exerted in mammalian sperm during fertilization. Several reports investigating cryopreservation-induced membrane changes in mammalian sperm have suggested that cryopreservation induces a compositional alteration of membrane rafts via a loss of membrane sterols, leading to impaired fertilizing ability. Recently, we demonstrated that membrane rafts are present in chicken sperm. Therefore, we investigated a possible mechanism for the induction of functional damage in cryopreserved chicken sperm, with particular attention to cryopreservation-induced compositional changes in membrane rafts. Sterol quantification showed that loss of sterols from sperm membranes occurred following cryopreservation. Biochemical analyses of detergent-insoluble membranes showed that the lipid and protein compositions of membrane rafts were altered dramatically by cryopreservation. To determine the physiological role of these changes, we examined external translocation of phosphatidylserine (PS), representing an early apoptotic change, and found that cryopreservation induced apoptotic changes in chicken sperm. Furthermore, methyl-β-cyclodextrin-induced loss of sterols from the plasma membranes stimulated PS translocation that was not accompanied with caspase-3 activation, which plays an important role downstream of the apoptotic cascade. Based on the results obtained in this study, we discuss a new mechanism for reduction of the fertilizing ability in avian sperm after cryopreservation.

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“…Among the factors responsible for generating injury during cryopreservation is oxidative stress (Zribi et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

“…Trans-resveratrol and quercetin have gained attention for their high antioxidant potential (Sarlós et al, 2002;Zribi et al, 2012). Transresveratrol is a non-flavonoid phenolic compound that belongs to the stilbenes family (Planas et al, 2011), whereas quercetin is an aglycone flavonoid of the flavonols subclass (Kelly, 2011).…”

Section: Introductionmentioning

confidence: 99%

High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

Silva

Arruda

Silva

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the effect of different concentrations of trans-resveratrol or quercetin on the ability of goat sperm to withstand being frozen. Six pools of semen obtained from six male goats were processed with different concentrations of resveratrol or quercetin (Experiment 1: 0, 15, 25, 50, 75 or 100µM resveratrol; Experiment 2: 0, 15, 25, 50, 75 or 100µM quercetin) and frozen. After thawing, the semen was evaluated for sperm kinematics, plasma membrane and acrosome integrity, morphology and oxidative stress following 0 and 1h of incubation. Immediately after thawing (0h), wobble (oscillation index) in the groups treated with 100µM of quercetin or resveratrol was lower (P<0.05) than in those treated with 0 and 25µM resveratrol and 0µM quercetin, respectively. After 1h of incubation, the total motility in treatments with 15, 50 and 75µM quercetin, as well as the plasma membrane integrity in all quercetin concentrations were lower (P<0.05) than at 0h. In opposition, the linearity of semen samples treated with 100µM quercetin and the straightness of those treated with 75 and 100µM quercetin were lower (P<0.05) at 0h than at 1h after thawing. Thus, it can be concluded that resveratrol and quercetin at high concentrations (100µM) transiently reduce the wobble of goat sperm submitted to frozen storage, and that quercetin (75 and 100µM) increases the linearity and straightness over time, which can be favorable for fertility.

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Effect of freezing–thawing process and quercetin on human sperm survival and DNA integrity

Cited by 113 publications

References 29 publications

Effect of sperm storage and selection techniques on sperm parameters

Effect of sperm storage and selection techniques on sperm parameters

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken

High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

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