Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro

Helgeland, Kristen

doi:10.1111/j.1600-0722.1976.tb00490.x

Cited by 15 publications

(14 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, F − -mediated decrease in protein synthesis and/or secretion has been well-documented [27], [28], [32], [60], [61], [62], [63], [64], [65], [66], [67], [68], [69], [70], [71], [72], [73]. Importantly, we demonstrated a decrease in enamel matrix transcripts during the maturation stage.…”

Section: Discussionsupporting

confidence: 62%

The Acid Test of Fluoride: How pH Modulates Toxicity

Sharma¹,

Tsuchiya

Skobe³

et al. 2010

PLoS ONE

View full text Add to dashboard Cite

BackgroundIt is not known why the ameloblasts responsible for dental enamel formation are uniquely sensitive to fluoride (F−). Herein, we present a novel theory with supporting data to show that the low pH environment of maturating stage ameloblasts enhances their sensitivity to a given dose of F−. Enamel formation is initiated in a neutral pH environment (secretory stage); however, the pH can fall to below 6.0 as most of the mineral precipitates (maturation stage). Low pH can facilitate entry of F− into cells. Here, we asked if F− was more toxic at low pH, as measured by increased cell stress and decreased cell function.Methodology/Principal FindingsTreatment of ameloblast-derived LS8 cells with F− at low pH reduced the threshold dose of F− required to phosphorylate stress-related proteins, PERK, eIF2α, JNK and c-jun. To assess protein secretion, LS8 cells were stably transduced with a secreted reporter, Gaussia luciferase, and secretion was quantified as a function of F− dose and pH. Luciferase secretion significantly decreased within 2 hr of F− treatment at low pH versus neutral pH, indicating increased functional toxicity. Rats given 100 ppm F− in their drinking water exhibited increased stress-mediated phosphorylation of eIF2α in maturation stage ameloblasts (pH<6.0) as compared to secretory stage ameloblasts (pH∼7.2). Intriguingly, F−-treated rats demonstrated a striking decrease in transcripts expressed during the maturation stage of enamel development (Klk4 and Amtn). In contrast, the expression of secretory stage genes, AmelX, Ambn, Enam and Mmp20, was unaffected.ConclusionsThe low pH environment of maturation stage ameloblasts facilitates the uptake of F−, causing increased cell stress that compromises ameloblast function, resulting in dental fluorosis.

show abstract

Section: Discussionsupporting

confidence: 62%

The Acid Test of Fluoride: How pH Modulates Toxicity

Sharma¹,

Tsuchiya

Skobe³

et al. 2010

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Fluoride toxication affects protein synthesis by primary causing destruction of polypeptide chains [34] and weakness of amino-acid bindings in proteins [35]. It has been reported that excessive amount of fluoride cause a reduction of protein synthesis [31] and also metabolisms of cholesterol and triglyceride in liver are affected in fluoride-exposed group [36].…”

Section: Naf Groupmentioning

confidence: 99%

Effect of Fluoride Exposure on Serum Glycoprotein Pattern and Sialic Acid Level in Rabbits

Çiftçi

Çenesiz

Yarım

et al. 2009

Biol Trace Elem Res

View full text Add to dashboard Cite

This study describes the effects of fluoride exposure on the protein profile, glycoprotein pattern, and total sialic acid concentration of serum in rabbits. For this aim; 20 healthy New Zealand rabbits were used. The rabbits were divided into two equal groups each with ten animals according to their weighing: control group and experimental group. The rabbits in control group were given drinking tap water containing 0.29 mg/l sodium fluoride and experimental group received the same tap water to which was added 40 mg/l sodium fluoride for 70 days. Blood samples were taken from each rabbit on day 70. Serum fluoride concentrations were measured by a fluoride-specific ion electrode in serum. The fluoride levels in the serum were found as 18.4 (±1.58) μg/L in control and 301.3 (±52.18) μg/L in fluoride exposed rabbits. The sialic acid levels were found as 69.2 (±0.32) mg/dL in control and 43.4 (±0.13) mg/dL in fluoride exposed group. The electrophoretic patterns of serum proteins, glycoproteins, and total sialic acid concentration were determined. Fifteen different protein fractions with molecular weights ranging from 22 to 249 kDa were displayed in the serum protein electrophoretic gel of both groups. The raw concentrations of the protein fractions decreased in fluoride exposed rabbits as compared with the control rabbits. The serum glycoprotein pattern revealed seven major protein bands from 47 to 167 kDa in experimental and control groups. The slight decrease of raw concentration of the protein bands in glycoprotein pattern of serum was observed in fluoride toxication comparing to control. The

show abstract

“…It was concluded that F was an inhibitor of protein synthesis, thus inducing growth inhibition [26]. Other data report that collagen metabolism was affected by F in vivo [10] and in vitro [9]. The decrease of collagen synthesis has been explained by an impairment in cross-linking and maturation of collagen fibers associated with an increase in collagen degradation [10].…”

Section: Protein Synthesismentioning

confidence: 95%

“…The influence of F on the dentinal matrix components, especially type I collagen, has been studied previously. In rabbit dental pulps incubated with graded amounts of F, release of collagen was inhibited by about 50% [9]. Sharma [10] described that long-term ingestion of F interferes with the biosynthesis, maturation, and degradation of rabbit skin collagen.…”

mentioning

confidence: 98%

Selective inhibition of collagen synthesis by fluoride in human pulp fibroblastsIn Vitro

1993

View full text Add to dashboard Cite

Human dental pulp cells were cultured in fluoridated mediums (0, 1, 10, 25 ppm) in order to study the biological effect of the ion regarding the cellular metabolism: cell growth, alkaline phosphatase (ALP) activity, and protein synthesis. The results indicated a decrease of the cell growth at 25 ppm and a dose-dependent decrease of the ALP activity. Type I collagen immunoperoxidase staining, radioimmunoassay quantitation, and analysis of type I and III collagens mRNA levels showed an inhibition of collagen production and gene expression. In contrast, fibronectin production and gene expression were not affected by fluoride. The treatment did not influence the qualitative pattern of the different mRNA species. Of the three collagen chains, the alpha 1(I) was the most affected. These data suggest that fluoride does not exert a general depletive effect on human dental pulp cells but rather a selective inhibition on collagen production.

show abstract

Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro

Cited by 15 publications

References 37 publications

The Acid Test of Fluoride: How pH Modulates Toxicity

The Acid Test of Fluoride: How pH Modulates Toxicity

Effect of Fluoride Exposure on Serum Glycoprotein Pattern and Sialic Acid Level in Rabbits

Selective inhibition of collagen synthesis by fluoride in human pulp fibroblastsIn Vitro

Contact Info

Product

Resources

About