Effect of Fluoride Exposure on Serum Glycoprotein Pattern and Sialic Acid Level in Rabbits

Abstract

This study describes the effects of fluoride exposure on the protein profile, glycoprotein pattern, and total sialic acid concentration of serum in rabbits. For this aim; 20 healthy New Zealand rabbits were used. The rabbits were divided into two equal groups each with ten animals according to their weighing: control group and experimental group. The rabbits in control group were given drinking tap water containing 0.29 mg/l sodium fluoride and experimental group received the same tap water to which was added 40 mg/l sodium fluoride for 70 days. Blood samples were taken from each rabbit on day 70. Serum fluoride concentrations were measured by a fluoride-specific ion electrode in serum. The fluoride levels in the serum were found as 18.4 (±1.58) μg/L in control and 301.3 (±52.18) μg/L in fluoride exposed rabbits. The sialic acid levels were found as 69.2 (±0.32) mg/dL in control and 43.4 (±0.13) mg/dL in fluoride exposed group. The electrophoretic patterns of serum proteins, glycoproteins, and total sialic acid concentration were determined. Fifteen different protein fractions with molecular weights ranging from 22 to 249 kDa were displayed in the serum protein electrophoretic gel of both groups. The raw concentrations of the protein fractions decreased in fluoride exposed rabbits as compared with the control rabbits. The serum glycoprotein pattern revealed seven major protein bands from 47 to 167 kDa in experimental and control groups. The slight decrease of raw concentration of the protein bands in glycoprotein pattern of serum was observed in fluoride toxication comparing to control. The

The Role of Hesperetin in the Management of Diabetes Mellitus and Its Complications

“…Our results are finding in line with the study of reduced fucose by improved secretion of insulin in coumarin treated diabetic rats [14]. Sialic acid is the terminal residue of the oligosaccharide side chain of glycoproteins and widely occurs in the exposed positions of molecules like hormones, enzymes and also on tissues [15]. Various factors cause elevation in the concentration of plasma sialic acid.…”

Diyabetik Sıçan Karaciğerinde Pazı Ekstresinin Glikoprotein Bileşikleri ve İleri Oksidasyon Protein Ürün Seviyeleri Üzerindeki Koruyucu Etkisi

“…Sialic acid is derived from neuraminic acid and can be found in glycoproteins structures. It plays a role in intracellular signalling, protease resistance, cell-cell recognition, conformational stabilization, and protein targeting (26). The sialic acid levels of serum and tissues are seen as a marker in diabetes (28), in which, the amount of sialic acid increases.…”

Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis

“…The increase in TSA levels may be due to the release of sialic acids in the membrane of cells damaged by oxidative stress developing due to fluorosis (Ozcelik et al, 2014;Koçak et al, 2020b). There are studies indicating that serum TSA (Jha et al, 1982;Ciftci et al, 2010;Doğan et al, 2016), PSA (Sharma, 1983) and testicular TSA (Kaya et al, 2015) levels decrease in fluorosis patients.…”