Effect of Fluoride Concentration on Remineralization of Carious Enamel an in vitro pH-Cycling Study

Abstract

An in vitro pH-cycling experiment was carried out to investigate the effect of fluoride concentration on enamel demineralization and remineralization. Artificial caries lesions were formed in an acid-buffered solution and subjected daily to a 3-hour acid attack, a 5-min immersion in the test NaF solution (0, 1,250, 500, 1,000, 1,750 and 2,500 ppm F), and to 21 h in an artificial saliva. Changes in mineral content were assessed weekly for 5 weeks using microradiography/microdensitometry. The lesions in the control group (0 ppm F) and the 1-ppm F group demineralized. Remineralization was significantly higher in the 500-ppm F group compared to the 250-ppm F group. However, higher fluoride concentrations did not produce any further significant increase in remineralization. Laminations were apparent in lesions subjected to the 250- and 500-ppm F solutions.

In Vitro Evaluation of Enamel Microhardness after Application of Two Types of Fluoride Varnish

“…As mentioned earlier, pH cycling method by creating acidic challenges greatly helps in the simulation process of the mouth environment in the laboratory [14][15][16]. Nevertheless, 100% simulation cannot be expected because of the important and significant role of factors associated with remineralization process such as speed and flow rate of saliva and composition and buffering capacity of saliva [17,18].…”

The effect of casein phosphopeptide‐amorphous calcium phosphate on remineralization of artificial caries‐like lesions: an in vitro study

“…3,20,21 Artificial early carieslike lesions of enamel show all of the principal histological features of natural caries and have been successfully used to study the remineralization of enamel in vitro. 3,22 Artificial caries-like lesions of enamel are more homogeneously reproducible than natural lesions 23 and thus provide a reliable experimental model.…”

Análise in vitro da Influência da Aplicação Tópica de Fluoreto nas Propriedades Mecânicas de uma Resina Ortodôntica sob Ciclagem de pH

“…O modelo de ciclagem de pH consisti u em um ciclo diário com alternância entre entre saliva arti fi cial remineralizadora neutra (1,54 mmol/L de cálcio, 1,54 mmol/L de fosfato, 20 mmol/L de ácido acéti co e 0,308 g de acetato de amônia, ajustada para pH 7,0 com hidróxido de potássio) 16 e saliva arti fi cial desmineralizadora ácida (3 mmol/L de cálcio, 3 mmol/L de fosfato, 50 mL/L de ácido acéti co e 0,308 g de acetato de amônia, ajustada para pH 4,5 com hidróxido de sódio) 17 . Todos os corpos de prova dos grupos submeti dos à ciclagem de pH foram manti dos em saliva desmineralizadora durante 22 horas consecuti vas e, após lavagens em água deionizada, permaneceram em contato com saliva remineralizadora durante o período de 2 horas, completando o ciclo de 24 horas (Quadro 2) tomando-se por base modelo de ciclagem prévio 18 .…”