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Background:  The aims of this study were to investigate the efficacy of CPP‐ACP containing Tooth Mousse on the remineralization of enamel lesions and to compare its efficacy to that of a fluoride‐containing toothpaste.Methods:  Permanent teeth were placed in demineralizing solution for 96 hours to produce artificial caries‐like lesions 120–200 μm in depth. They were sectioned into 100–150 μm thick samples and randomly assigned to five groups: for Group A, a fluoridated toothpaste (1100 ppm) was used as a positive control and in Group B, a non‐fluoridated toothpaste was used as a negative control. Tooth Mousse containing CPP‐ACP was tested by three different means: as a toothpaste (Group C); as a topical coating (Group D); and (Group E) as a topical coating after treating the sections with the same fluoridated toothpaste as in Group A.Results:  The lesion depth decreased significantly by 7 per cent in Group A, 10.1 per cent in Groups C and D, and 13.1 per cent in Group E (Paired t‐ test, p < 0.05), while in Group B the lesion depth increased significantly by 23 per cent.Conclusions:  Based on the data obtained, CPP‐ACP containing Tooth Mousse remineralized initial enamel lesions and it showed a higher remineralizing potential when applied as a topical coating after the use of a fluoridated toothpaste.

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