Effect of feeding pattern and dietary protein source on protein synthesis in European sea bass (Dicentrarchus labrax)

Langar, Habib; Guillaume, J.

doi:10.1016/0300-9629(94)90119-8

Cited by 16 publications

(9 citation statements)

References 15 publications

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“…The higher protein synthesis rate was always accompanied by a greater degradation rate and lower efficiency of protein retention (Langar et al. 1993; Langar & Guillaume 1994).…”

Section: Whole Body and Liver Compositionmentioning

confidence: 99%

Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L.

et al. 2004

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The development of a test diet supporting good fish growth to be used in gilthead seabream indispensable amino acid (IAA) requirement studies based on dose-response relationship was the objective of the present study. Four isonitrogenous diets, one serving as control (diet C) based on fish meal LT, and three others in which the fish meal component was reduced to 45% and the rest of the nitrogen was provided by mixtures of crystalline AA were formulated. Diet S resembled the whole body IAA profile of wild seabream. Diet D was made deficient in all IAA and a 10% excess of IAA compared with diet S was used in diet E. Triplicate groups of fish were fed the diets to satiation for 6 weeks. Growth parameters and nutrient utilization efficiencies among diets C and S were similar, while excessive supplementation of IAA negatively affected most of them, compared with the control. Whole body composition was differentiated in relation to the diet fed. No significant differences were observed among groups fed the control and diet S. As shown by the results, the presence of crystalline AA in diet S does not significantly affect growth, and could therefore serve as control diet in IAA requirement studies of gilthead seabream. KEY WORDS

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“…The higher protein synthesis rate was always accompanied by a greater degradation rate and lower efficiency of protein retention (Langar et al. 1993; Langar & Guillaume 1994).…”

Section: Whole Body and Liver Compositionmentioning

confidence: 99%

Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L.

et al. 2004

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“…Diets that provide a poor amino acid balance tend to stimulate liver protein synthesis ( 8 ) and depress white muscle protein synthesis ( 7 ) . At the whole animal level, diets with poorer amino acid balance stimulated protein synthesis ( 9 , 10 ) ; this effect was because protein synthesis was measured at 3 h after feeding when, presumably, peak rates of liver protein synthesis accounted for a large proportion of total protein synthesised. At peak rates, liver protein synthesis can often account for most of whole-fish protein synthesis ( 34 ) .…”

Section: Discussionmentioning

confidence: 99%

“…Protein turnover reflects dietary protein in relation to how closely it matches quantitative and qualitative amino acid requirements ( 11 – 13 ) . Low or imbalanced amino acid supply tends to initially stimulate protein synthesis in the liver in order to maintain protein synthesis and growth in the skeletal muscle ( 9 ) . Prolonged feeding at a low intake of amino acids will down-regulate protein synthesis so that liver rates decrease to be the same or lower than on a higher-quality protein source and skeletal muscle protein synthesis cannot be sustained and will be depressed ( 11 , 14 ) .…”

mentioning

confidence: 99%

“…The principal aim of the present study was to assess postprandial protein synthesis as a sensitive test of nutritionally similar diets with different ingredient composition. Measurement of protein synthesis using a flooding dose of a labelled amino acid ( 25 ) has advanced the understanding of the relationships between diet composition, protein metabolism and nutrient utilisation in aquatic animals and is the method that has been used most extensively in fish ( 8 , 9 , 26 ) . However, the complexity of the method means that there are relatively few published studies on fish that compare diets ( 11 , 27 ) .…”

mentioning

confidence: 99%

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Protein synthesis in gilthead sea bream: response to partial fishmeal replacement

et al. 2012

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The present study aimed to measure tissue protein synthesis in sea bream fed isonitrogenous diets that contained 63, 55 and 50 % fishmeal; in the latter two diets, 16 and 27 % of the fishmeal protein was replaced with plant protein. Over a 35 d period, there were no differences in feed intake, growth or feed efficiency among the three diets. Protein metabolism was then measured in the liver and white muscle tissue as rates of protein synthesis and as the capacity for protein synthesis before feeding (0 h) and at different times after feeding (4-48 h). Diet did not have a significant effect on protein synthesis or on the capacity for protein synthesis in either tissue. The capacity for protein synthesis was not affected by time after feeding, and overall mean values were 81·02 (SE 1·68) and 4·07 (SE 0·94) mg RNA/g protein for the liver and white muscle, respectively. Liver and white muscle fractional rates of protein synthesis were significantly higher at 4 -8 h, intermediate at 12 h and were not different among pre-feeding (0 h), 24 and 48 h. Overall, the indices of protein metabolism measured at various times over 48 h following feeding were closely aligned with measurements of feeding, growth and growth efficiency established over a longer time scale. Key words: Protein synthesis: Specific dynamic action: Fishmeal replacementThe development of sustainable aquaculture is partly dependent on sustainable aquafeeds and the replacement of some or all of the protein and oil derived from marine sources (1 -3) . Strategies include the use of protein and oil sources derived from agriculture production as well as consideration of organic and certified ingredients that may include those of marine origin (4,5) . While considerable knowledge exists about the use of alternative protein sources in aquafeeds, the majority of this has been obtained from long-term growth experiments. Protein turnover is clearly of central importance to growth but only a few studies on fish have investigated protein turnover (6) , protein synthesis and protein degradation, in relation to dietary modification; even fewer have investigated the effect of replacing fishmeal on protein synthesis (7 -10) . Protein turnover reflects dietary protein in relation to how closely it matches quantitative and qualitative amino acid requirements (11 -13) .Low or imbalanced amino acid supply tends to initially stimulate protein synthesis in the liver in order to maintain protein synthesis and growth in the skeletal muscle (9) . Prolonged feeding at a low intake of amino acids will down-regulate protein synthesis so that liver rates decrease to be the same or lower than on a higher-quality protein source and skeletal muscle protein synthesis cannot be sustained and will be depressed (11,14) . When an alternative dietary protein is of high quality, muscle protein synthesis may not be affected by protein sources used, as in barramundi (Lates calcarifer) fed lupin meal to replace 45 % of the fishmeal protein (15) . In contrast, wholeanimal protein synthe...

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“…Previous studies of temperate marine species have measured protein metabolism in laboratory maintained animals, in most cases fed artificial diets (e.g. Houlihan et al, 1990;Houlihan et al, 1994;Langar and Guillaume, 1994;Meyer-Burgdorff and Rosenow, 1995;Sveier et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Growth in the slow lane: protein metabolism in the Antarctic limpetNacella concinna(Strebel 1908)

Fraser

Clarke

Peck

2007

Journal of Experimental Biology

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2=28.8%, P<0.05). In turn, this suggests that temperature may be an important factor in determining ectotherm growth efficiency via an influence on PSRE. Maximal fractional and absolute protein synthesis rates occurred at ~1°C in N. concinna, the approximate summer water temperature at the study site, and protein synthesis rates decreased above this temperature. In the absence of adaptation, predicted increases in Antarctic water temperatures would result in reduced, rather than increased, rates of protein synthesis and, in turn, possibly growth.

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Effect of feeding pattern and dietary protein source on protein synthesis in European sea bass (Dicentrarchus labrax)

Cited by 16 publications

References 15 publications

Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L.

Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L.

Protein synthesis in gilthead sea bream: response to partial fishmeal replacement

Growth in the slow lane: protein metabolism in the Antarctic limpetNacella concinna(Strebel 1908)

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