Effect of environmental factors on PCR-DNA analysis from dental pulp

García, A. Alvarez; Muñoz, Irene; Pestoni, C.; Lareu, M.V.; Rodriguez‐Calvo, María Sol; Carracedo, Ángel

doi:10.1007/bf01369671

Cited by 93 publications

(51 citation statements)

References 8 publications

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“…The teeth were divided into five different experimental conditions: immersion in sweet and sea water, stored approximately 10 cm deep in garden soil, kept at room temperature, and finally included in cotton with alcohol and burned [2]. The teeth are placed for periods ranging from 1 week to 2 years.…”

Section: Sample and Sample Preparationmentioning

confidence: 99%

The DNA extraction from the pulp dentine complex of both with and without carious

Corte‐Real

Andrade

Anjos

et al. 2006

International Congress Series

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Abstract. The main objective of this paper is to show that, despite some adverse forensic condition such as degraded human body remains and exhumed material, the dentine (in pulp dentine complex) keeps, in the majority of cases, its integrity. In this study, we use a sample of 30 human teeth (both with and without carious) after extraction during dental treatment. We analyze 15 STRs and both high-variable regions I and II of mitochondrial DNA. Each tooth was prepared using a technique that comprises the mechanic removal of the enamel, central pulp and cement. The DNA extraction was carried out with a commercial kit, but the protocol was adjusted according to the specificities of the sample. This procedure has allowed us to obtain a genetic profile of mitochondria DNA in all the samples as well as to define a profile of STRs in some of them. D

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Section: Sample and Sample Preparationmentioning

confidence: 99%

The DNA extraction from the pulp dentine complex of both with and without carious

Corte‐Real

Andrade

Anjos

et al. 2006

International Congress Series

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“…A study by Alvaraz Garcia et al 32 used the homologous gene amelogenin to determine sex in dental pulp subjected to various environmental factors.…”

Section: Arşiv Kaynak Tarama Dergisi Archives Medical Review Journalmentioning

confidence: 99%

İnsan İskeleti Parçalarından ve Diş Örneklerinden Amelogenin Genini Kullanarak Cinsiyet Belirleme

Zagga¹,

Ahmed²,

Tadros³

et al. 2014

Arşiv Kaynak Tarama Dergisi

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Alternative approaches to sex determination of DNA samples involve investigation of regions of the amelogenin gene. This is the gene that encodes tooth enamel and is present on both the X and Y chromosomes. A review composed via Medline Internet search of literature and contributions from our experiences as well as experiences from colleagues. The rareness of failures in sex determination provides confidence in current techniques, but amelogenin gene method (singly) of sex determination is not without failures. Amelogenin PCR method/system of sex determination should not, at the moment, completely replace traditional methods of sex identification. Hence, sex identification with amelogenin gene, of subjects for forensic purposes should be conducted as much as possible through a multiple morphological-molecular combined methods to avoid fallibility of amelogenin gene.

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“…Among old and putrefied or degraded tissues, DNA in teeth seems to show the best preservation (Schwartz et al 1991). The pulp is well protected by the dental hard tissues and contains different kinds of cells (Alvarez Garcia et al 1996). The dental cement covering the dentin of the roots is produced by DNA-containing cementoblasts which are concentrated in the lower third of the apex and in the area of the bifurcatio of teeth with more than one root (Smith et al 1993).…”

Section: Introductionmentioning

confidence: 99%