The DNA extraction from the pulp dentine complex of both with and without carious

Corte‐Real, Ana; Andrade, L.; Anjos, M.J.; Carvalho, M.; Vide, M.C.; Côrte-Real, F.; Vieira, Duarte Nuno

doi:10.1016/j.ics.2005.11.053

Cited by 16 publications

(13 citation statements)

References 5 publications

(4 reference statements)

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“…Initially it was thought that the genetic analysis of the tooth could only be made from the pulp tissue [1,7]. Recently the analysis from its hard tissues has became more relevant [2][3][4]. The first choice is always for the intact and healthy tooth, however this option is not always available [2].…”

Section: Discussionmentioning

confidence: 99%

“…The endodontic filling was removed using a fine spoon excavator and the root channels were cleaned. The remaining hard tissues were isolated and reduced to powder in cryogenic mill [2][3][4].…”

Section: Methodsmentioning

confidence: 99%

“…During an endodontic procedure the pulp tissue and the inner layer of dentine are removed (the major contribute of nuclear DNA [1,2]) and replaced for endodontic filling usually thermoplastic and cement. Into the dentinal tubules, the odontoblats prolongations are preserved from the aggressions (mitochondrial DNA) [2].…”

Section: Introductionmentioning

confidence: 99%

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Genetic identification in endodontic treated tooth root

Corte‐Real

Anjos

Andrade

et al. 2008

Forensic Science International: Genetics Supplement Series

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The tooth is an important sample in adverse forensic conditions. Its morphology and histology contributes for the preservation of the cellular components. The endodontic root filling is a methodology used for preservation of tooth, in life, supporting a fixed rehabilitation. During endodontic procedure the pulp tissue and the inner layer of dentine are removed (the major nuclear DNA) and replaced for endodontic filling usually thermoplastic and cement. In the dentinal tubules, the odontoblats prolongations are preserved (mitochondrial DNA). In this paper were studied endodontic tooth and the respective donor blood on compresses as reference samples. The samples were prepared. Teeth DNA extraction was made with a commercial kit, Purigene 1 DNA Purification System (PE Gentra), and quantified with an ABI Prism 1 7000; amplification was performed with Identifiler TM PCR Amplification Kit (Applied Biosystems). In samples without STR profile was sequenced HVRI and HVRII. The samples were genotyped using an ABI Prism 1 310 and 3130 Analysers. Results showed that with endodontic treated teeth were obtained STR profiles. Teeth are extraordinary samples in identification caseworks. This study showed that even in roots with endodontic treatment were obtained full STR profiles, giving the possibility of individual genetic identification.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Genetic identification in endodontic treated tooth root

Corte‐Real

Anjos

Andrade

et al. 2008

Forensic Science International: Genetics Supplement Series

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“…Microanatomy plays a vital role in the extraction of DNA, which results in the identification of degraded human beings [17]. The adverse conditions of the degraded human remain, and the DNA extraction is carried out by adjusting the technique to remove enamel, cementum, and pulp [18]. The mtDNA displays a pattern of fragmentation across many tissue types, improving the ability to estimate DNA survival in forensic specimens [19].…”

Section: Review 1mentioning

confidence: 99%

Forensic Odontology, a Boon and a Humanitarian Tool: A Literature Review

Kaleelullah

Hamid

2020

Cureus

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Our society faces fresh challenges in every conceivable way, from an increase in the crime rate to a rise in natural disasters. In spite of the leaps in modern technology, medical breakthroughs in the identification of criminals remain a cumbersome task. The modern-day criminal investigation typically involves many different disciplines to solve a crime. The identification of a person's body is required when it is completely mutilated, disfigured, or beyond recognition-which might be due to any barbaric crime, accident, war, fire, or any natural disaster. Dental identification is one of the most reliable methods, as teeth and dental structures may survive adverse conditions. The techniques involved in forensic odontology include: bite mark analysis, tooth prints, rugroscopy, cheiloscopy, dental DNA analysis, radiographs, and photographs.

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“…Furthermore, an abundance of quality DNA can be extracted from a tooth which is an important advantage in DNA analysis. 31,32 6. Sampling of the tooth for DNA extraction Various methods have been reported regarding the extraction of DNA from the tooth which includes sectioning of teeth horizontally at the cemento-enamel junction or vertically up to root tip, scraping and aspiration.…”

Section: Teeth and Dna Analysismentioning

confidence: 99%