Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Abstract

Abstract. The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimizations of physical and chemical parameters were done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 -9), incubation temperature (20 -30 °C), incubation time (8 -80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

Effect of inoculum size, inducer and metal ion on lipase production by Rhodococcus strain UCC 0009

“…A couple of studies supported the present research finding, which indicated olive oil as the most optimum inducer to aid the secretion of lipase from the local isolate. Improved lipase production from Aspergillus niger was observed when 5 % (v/v) and 1 % (v/v) olive oil were used as the inducer [22,23], which coincided with the outcomes from the present study. However, a study established coconut oil as the inducer and sole carbon source in promoting the highest lipase activity from Aspergillus tamarii.…”

“…Natural oils, namely olive oil, canola oil, coconut oil, corn oil, sunflower oil, sesame oil, and mustard oil, are often utilized as inducers for the secretion of the enzyme lipase. A high lipase yield can also be acquired from lipidic carbon sources comprising of fats and vegetable oils, which are regarded as principal sources of lipidic carbon [22].…”

“…Two factors positively influence lipase production as an inducible enzyme, namely the type and concentration of lipid sources in the fermentation media, such as fatty acids [23]. Olive oil emerged as the best inducer for lipase production in Rhodococcus strain UCC 009 since it contains high oleic acid content, which most lipases are specific towards, and low linoleic acid content, which have been reported to have a detrimental impact on lipase productivity [22]. A couple of studies supported the present research finding, which indicated olive oil as the most optimum inducer to aid the secretion of lipase from the local isolate.…”

Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent

“…Further, previous studies by Mukhtar et al [48] and El-Batal et al [49] indicated that olive oil exhibits good inductive effect, whereas the present findings showed repressive effect. However, our results are in accordance with studies by Malilas et al [50], who reported the lipase production by eight fungal strains using olive oil as an inducer in SmF, which showed no noticeable lipase activity from Fusarium solani after seven days of incubation.…”

Functionalized polymeric silver nanoparticle hybrid network as a dual antimicrobe:Synthesis, characterization, and antibacterial application

“…1,2 Thus, the scientific community gives much attention to the discovery of new and cost-effective antimicrobial agents. 3 But wide use of these agents leads to antibiotic resistance, which in turn becomes a serious issue for human health. 4 Altering the structure of bacteria and using dual-action antimicrobes are the most common techniques applied to overcome bacterial resistance.…”