Effect of enamel matrix proteins on the periodontal connective tissue‐material interface after wound healing

Craig, Ronald G.; Kallur, Sathya P.; Inoue, Masahiro; Rosenberg, Paul A.; LeGeros, Racquel Z.

doi:10.1002/jbm.a.20140

Cited by 21 publications

(13 citation statements)

References 38 publications

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“…This is in agreement with in vivo findings that showed no expression of amelogenin during cementogenesis . There is some preliminary evidence that EMPs could induce cementum formation on materials that do not normally support connective tissue adhesion . Thus, it appears that EMPs could target mainly fibroblastic cells and their differentiation rather than cementogenesis itself.…”

Section: Enamel Matrix Proteins In Periodontal Regeneration and Woundsupporting

confidence: 90%

Biological agents and cell therapies in periodontal regeneration

Larjava¹,

Yang²,

Putnins³

et al. 2012

Endodontic Topics

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Regeneration of periodontal structures is a complex challenge for periodontal wound healing. As by Wikesjö et al. in this issue, wound space provision, primary stability, and healing by primary intention serve as critical factors for attempts to regenerate the periodontium. Barrier membranes for space maintenance can be cumbersome to use in a clinical setting and have proven to provide only partial success in regenerative therapies. Not surprisingly, investigators and clinicians have started to search for biological molecules and cell technologies that could help to either speed up or modify the healing process that depends on migration and differentiation of several different cell populations. In this chapter, we will briefly review the technologies that are currently in use or in clinical trials. Readers are welcome to review in‐depth publications for further details on this complex and evolving area of research.

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Section: Enamel Matrix Proteins In Periodontal Regeneration and Woundsupporting

confidence: 90%

Biological agents and cell therapies in periodontal regeneration

Larjava¹,

Yang²,

Putnins³

et al. 2012

Endodontic Topics

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“…Indeed, the formation of a mineralized matrix on calcium hydroxide and gutta percha surfaces with inserting fibers from the periodontal ligament in a porcine experimental model is reported in an accompanying article. 40 The mechanism through which EMD regulates PDL cell protein synthesis has not been characterized. Attempts to identify known growth factors or cytokines in EMD to possibly account the effects of EMD on protein synthesis have not been successful.…”

Section: Discussionmentioning

confidence: 99%

Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

Inoue

LeGeros

Hoffman

et al. 2004

J Biomedical Materials Res

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Cells within the periodontal ligament have the potential to regenerate a periodontal connective tissue attachment on pathologically exposed root surfaces as well as on several material surfaces including titanium. However, rather than a periodontal connective tissue attachment, a fibrous encapsulation or chronic inflammatory response has been reported at the material connective tissue interface for most dental materials. Cementum is the first tissue of the periodontal connective tissue attachment to develop and the secretion of enamel matrix related proteins on the newly mineralized dentin surface precedes and is thought to induce cementum formation. Enamel matrix-related proteins may also function in the adult because the application of an acid extract of porcine enamel protein matrix (Emdogain(R), EMD) on pathologically exposed root surfaces has been shown to result in cementum regeneration. Therefore, the objective of the present study was to determine whether the application of EMD to materials that do not normally support cementogenesis in vivo would alter the in vitro phenotype of periodontal ligament (PDL) cells including the synthesis of cementum-associated extracellular matrix proteins. Primary PDL cells were established from 21-day-old Sprague-Dawley rats, and were cultured on four materials commonly encountered in dental practice (gutta percha, calcium hydroxide, amalgam, and super EBA cement) with and without the application of EMD. After 7 or 14 days of culture, total-DNA content, collagen synthesis, alkaline phosphatase activity, and the synthesis of a 42-kDa cementum-associated extracellular matrix protein were determined. PDL cells cultured on all materials had decreased total DNA content. The application of EMD further decreased total DNA content. PDL cells cultured on gutta percha and calcium hydroxide with the application of EMD had similar levels of collagen synthesis and alkaline phosphatase activity but also expressed a 42-kDa cementum extracellular matrix-associated protein when compared to the other groups. These results suggest that EMD can alter the phenotype of PDL cells when cultured on these dental materials.

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“…We also used this animal model to induce atrophy of the parotid glands in order to study the effect of these glands on body nitrate metabolism (Xia et al , 2003b). The miniature pig has also been used for testing implant materials and techniques (Schultze‐Mosgau et al , 2000; Buchter et al , 2005; Nkenke et al , 2005), for reconstruction of craniofacial and temporal mandibular joint fractures (Bermejo et al , 1993; Niederhagen et al , 1999; Hollister et al , 2000; Wurzler et al , 2004), for studies on the biological properties of the periodontal tissues and the treatment of periodontal diseases (Kalkwarf and Krejci, 1983; Craig et al , 2004; Ziegler et al , 2005), and on the therapy of obstructive sleep apnea syndrome (Chen et al , 2000; Li et al , 2005a).…”

Section: Other Applicationsmentioning

confidence: 99%

The miniature pig: a useful large animal model for dental and orofacial research

et al. 2007

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Compared with small animal models such as rodents, large animal models are superior in many aspects for the study of human diseases and pre-clinical therapies. Since the development of the Minnesota miniature pig in 1949 at the Hormel Institute (USA), miniature pigs have been used as a large animal model in medical studies for scientific, economic, and ethical reasons. The oral maxillofacial region of miniature pigs is similar to that of humans in anatomy, development, physiology, pathophysiology, and disease occurrence. In this review, we describe the anatomical characteristics of the oral maxillofacial system of the miniature pig, established models of oral diseases in this animal, and other uses of the miniature pig in orofacial research.

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Effect of enamel matrix proteins on the periodontal connective tissue‐material interface after wound healing

Cited by 21 publications

References 38 publications

Biological agents and cell therapies in periodontal regeneration

Biological agents and cell therapies in periodontal regeneration

Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

The miniature pig: a useful large animal model for dental and orofacial research

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