Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials

Inoue, Miho; LeGeros, Racquel Z.; Hoffman, Carrie; Diamond, K.; Rosenberg, Paul A.; Craig, Ronald G.

doi:10.1002/jbm.a.20139

Cited by 10 publications

(11 citation statements)

References 42 publications

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“…21 However, the application of EMD to these substrates permitted the expression of the cementum associated protein on gutta percha and calcium hydroxide surfaces by primary periodontal ligament cell cultures. 22 Therefore, the objective of the present study was to determine whether EMD applied to surfaces not reported to be conducive to cementogenesis in vivo, would alter the type of material-connective tissue interface obtained after wound healing using an experimental animal model.…”

Section: Discussionmentioning

confidence: 99%

“…6,7 However, in a companion article, we report that EMD applied to gutta percha or calcium hydroxide permitted the expression of the cementum extracellular matrix protein by primary periodontal ligament cells after 7 days of culture. 22 These results suggest that the application of EMD can alter the phenotype of periodontal ligament cells when cultured on these materials. In this study, we have extended these findings and determine whether the application of EMD to materials previously reported not supportive of periodontal connective attachment formation would alter the type of periodontal connective tissue material interface, including the formation of cementum, after wound healing in an experimental animal model.…”

Section: Introductionmentioning

confidence: 92%

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Effect of enamel matrix proteins on the periodontal connective tissue‐material interface after wound healing

Craig

Kallur

Inoue

et al. 2004

J Biomedical Materials Res

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The periodontal ligament has the potential to regenerate a complete periodontal connective tissue attachment, starting with the deposition of cementum, on pathologically exposed root surfaces as well as several materials including titanium oxide. However, most commonly used dental materials result in a fibrous encapsulation or a chronic inflammatory response after periodontal wound healing rather than the formation of a periodontal connective tissue attachment. Recently, an extract of porcine enamel matrix (Emdogain(R), EMD) has been reported inductive of cementum formation in both in vivo and in vitro studies. The aim of this study was to determine the effect of EMD, when applied to materials previously reported not supportive of periodontal connective tissue formation, on the periodontal connective tissue-material interface obtained with these materials in vivo. Bilateral osteotomies were performed on the mandible of a Yucatan minipig exposing the buccal root surface of four premolars. A series of four preparations were placed in each root surface that were subsequently filled with calcium hydroxide, gutta percha, mineral trioxide aggregate (MTA), or left unfilled. One side, in addition, received an application of EMD prior to surgical closure. A bioabsorbable surgical barrier membrane was placed over the osteotomy sites to exclude gingival connective tissue from the wound-healing environment. The mucoperiosteal flaps were then readapted and sutured in position. The animal was euthanized 10 weeks after the procedure, block sections obtained and prepared for light microscopy. Results demonstrated complete regeneration of alveolar bone and periodontal ligament in all four teeth from the EMD-treated side. Fibers from the periodontal ligament were observed to insert into a mineralized matrix consistent with cementum on all four root preparations. In contrast, massive root resorption without regeneration of alveolar bone was found on all teeth from the side not treated with EMD. The results of this pilot study suggest that the application of EMD to material surfaces that normally do not support periodontal connective tissue attachment formation can alter the type of periodontal connective tissue interface obtained with these materials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Effect of enamel matrix proteins on the periodontal connective tissue‐material interface after wound healing

Craig

Kallur

Inoue

et al. 2004

J Biomedical Materials Res

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“…EMD stimulates mineralized tissue formation by modulating regulatory molecules in fibroblasts, a process crucial to bone metabolism (52). Furthermore, EMD alters phenotype markers of PDL fibroblasts when cultured on gutta-percha and calcium hydroxide (75).…”

Section: Emdogain and Fibroblastsmentioning

confidence: 99%

Emdogain® in carcinogenesis: a systematic review of in vitro studies

2010

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Emdogain is a commercial product of unknown composition and is clinically used to induce periodontal regeneration. This study aims to review current knowledge of the in vitro effects of Emdogain on oral tissues and, in particular, factors related to carcinoma. A systematic approach was used to review studies from the Embase and Pubmed databases; a total of 76 studies were included. These comprised in vitro studies of the cytokines in, or regulated by, Emdogain and assays designed to study the effects of EMD on human cells in oral tissues or malignant cells. Several studies have shown that EMD regulates the proliferation, migration, adhesion, gene expression, and cytokine production of (pre-)osteoblasts, periodontal fibroblasts, and gingival fibroblasts. However, the effects of EMD on malignant oral cells are not well understood. EMD seems to have broad regulatory effects on malignant cells and on several carcinoma-related factors. Evidence suggests that patients with premalignant or malignant mucosal lesions should not be treated with EMD. (J Oral Sci 52, 1-11, 2010)

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“…Increased surface roughness is achieved by blasting the surface with titanium oxide, analytical etching, sand blasting and acid etching 3 . One of the main aims of materials research for implantology is study and development of 110 V. Pivodova, J. Frankova, J. Ulrichova macromolecules, such as collagens, glycosaminoglycans and various non-collagenous proteins [7][8][9] . An important function of the root cementum is to invest and anchor the principal periodontal ligament fibres which spin like a meshwork between the root and alveolar bone, to the root.…”

Section: Introductionmentioning

confidence: 99%