Effect of emulsification-diffusion parameters on the formation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) particles

Göz, Eda; Karakeçili, Ayşe

doi:10.3109/21691401.2014.937869

Cited by 8 publications

(1 citation statement)

References 29 publications

(52 reference statements)

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“…Type A gelatin was used to fabricate BMP-9-loaded GMs by an emulsion-solvent diffusion method 14) . Briefly, a 25 wt% gelatin solution prepared in 30 ml of liquid paraffin was subjected to crosslinking in glutaraldehyde aqueous solution at 4°C for 24 h, followed by quenching with glycine.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Biocompatible nHA/Col-BMP-9/GM Scaffold: Synthesis, Characterization, and Effects on Bone Marrow Mesenchymal Stem Cells

Zhang

et al. 2019

J. Hard Tissue Biology.

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Bone tissue engineering is a complex process that requires concerted functions of biomaterials, cells, and bioactive growth factors. A porous nano-hydroxyapatite (nHA)/collagen (Col)-bone morphogenetic protein (BMP)-9/gelatin microsphere (GM) scaffold was fabricated by a combination of blending, crosslinking, and freeze-drying technologies. The nHA/Col-BMP-9/GM scaffold was well manufactured with a three-dimensional structure and had good mechanical properties. Rat bone marrow mesenchymal stem cells (BMMSCs) were isolated and cultured in vitro, and then identified by flow cytometry. An indirect Cell Counting Kit-8 assay demonstrated that the scaffolds favored BMMSC proliferation, indicating their cytocompatibility. In vitro, BMMSCs were seeded onto the nHA/Col-BMP-9/GM scaffolds to mimic a natural microenvironment. Scanning electron microscopy observation and adhesion rate determination were performed to evaluate the adherence and growth of BMMSCs on the composite scaffolds. Cells cultured in the scaffolds tended to maintain good morphology, attachment, and proliferation, confirming that the scaffolds were biocompatible and showed no cytotoxicity in vitro. Alkaline phosphatase expression was examined to assess the effect of the composite scaffolds on the osteogenic differentiation of rat BMMSCs. The results revealed that the nHA/Col-GM scaffolds, especially the BMP-9-loaded nHA/Col-GM scaffolds, significantly promoted the osteogenic differentiation of BMMSCs. For in vivo determination, acute toxicity, pyrogenic, and implantation tests were performed and verified that the composite scaffolds possessed excellent biocompatibility. This novel biocompatible scaffold has good application prospects for bone repair and regeneration.

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Section: Scaffold Fabricationmentioning

confidence: 99%

Biocompatible nHA/Col-BMP-9/GM Scaffold: Synthesis, Characterization, and Effects on Bone Marrow Mesenchymal Stem Cells

Zhang

et al. 2019

J. Hard Tissue Biology.

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Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation

Demirtaş

Göz

Karakeçili

et al. 2015

J Mater Sci: Mater Med

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Natural microenvironment during bone tissue regeneration involves integration of multiple biological growth factors which regulate mitogenic activities and differentiation to induce bone repair. Among them platelet derived growth factor (PDGF-BB) and bone morphogenic protein-6 (BMP-6) are known to play a prominent role. The aim of this study was to investigate the benefits of combined delivery of PDGF-BB and BMP-6 on proliferation and osteoblastic differentiation of MC3T3-E1 preosteoblastic cells. PDGF-BB and BMP-6 were loaded in gelatin and poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) particles, respectively. The carrier particles were then loaded into 3D chitosan matrix fabricated by freeze drying. The fast release of PDGF-BB during 7 days was accompanied by slower and prolonged release of BMP-6. The premising release of mitogenic factor PDGF-BB resulted in an increased MC3T3-E1 cell population seeded on chitosan scaffolds. Osteogenic markers of RunX2, Col 1, OPN were higher on chitosan scaffolds loaded with growth factors either individually or in combination. However, OCN expression and bone mineral formation were prominent on chitosan scaffolds incorporating PDGF-BB and BMP-6 as a combination.

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Preparation and characterization of nano and micro particles of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) via emulsification/solvent evaporation and nanoprecipitation techniques

et al. 2018

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Effect of emulsification-diffusion parameters on the formation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) particles

Cited by 8 publications

References 29 publications

Biocompatible nHA/Col-BMP-9/GM Scaffold: Synthesis, Characterization, and Effects on Bone Marrow Mesenchymal Stem Cells

Biocompatible nHA/Col-BMP-9/GM Scaffold: Synthesis, Characterization, and Effects on Bone Marrow Mesenchymal Stem Cells

Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation

Preparation and characterization of nano and micro particles of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) via emulsification/solvent evaporation and nanoprecipitation techniques

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