Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation

Demirtaş, Tuǧrul Tolga; Göz, Eda; Karakeçili, Ayşe; Gümüşderelı́oğlu, Menemşe

doi:10.1007/s10856-015-5626-9

Cited by 21 publications

(6 citation statements)

References 31 publications

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“…Furthermore, PDGF induces osteoblast differentiation by enhancing collagen synthesis and bone cell proliferation as well as tissue repair [17,18]. Demirtas et al [19] reported that isolated PDGF induced two-fold proliferation of osteoblasts in comparison with the control in vitro. In the current study, both fresh-PRP and FD-PRP has similar effects.…”

Section: Discussionmentioning

confidence: 99%

Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction

et al. 2020

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This study aimed to evaluate the in vitro pharmacological activity of growth factors (GFs) in freeze-dried platelet-rich plasma (FD-PRP) after storage for 4 weeks. Overview of Literature: Freshly prepared PRP is a rich source of many GFs. We reported that FD-PRP stored for 8 weeks accelerated bone union in a rat posterolateral fusion model equally well as fresh-PRP. However, the pharmacological activity of FD-PRP after longterm storage has not been shown in vitro. Methods: Immediately after preparation, as well as 4 weeks after freeze-dried storage, the platelet count was measured. Human osteoblasts were treated with fresh-PRP and FD-PRP, respectively. Western blotting was used to assess the phosphorylation of the platelet-derived growth factor (PDGF) receptor (PDGFR) and its downstream target, extracellular signal-regulated kinase (ERK). The proliferation rates of osteoblasts were investigated by immunocytochemistry and MTT cell viability assays. Furthermore, we used western blotting to evaluate the effect of PDGFR knockdown on the phosphorylation of ERK stimulated with fresh-PRP and FD-PRP. Results: Platelet counts in both the fresh-PRP and FD-PRP samples were approximately 10-fold higher than in peripheral blood samples. The phosphorylation and activation of the PDGFR and ERK were evenly induced by fresh-PRP and FD-PRP stimulation. Both fresh-PRP and FD-PRP significantly induced osteoblast proliferation in MTT cell viability assays. Furthermore, osteoblast PDGFR knockdown attenuated the downstream ERK activation by fresh PRP and FD-PRP. Conclusions: We demonstrated the pharmacological activity of PDGF in FD-PRP in vitro after 4 weeks of storage.

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Section: Discussionmentioning

confidence: 99%

Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction

et al. 2020

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“…SP-1 P recruits osteoblast precursors ( Meshcheryakova et al, 2017 ), promotes osteoblast proliferation, differentiation, activity, and survival ( Higashi et al, 2016 ; Meshcheryakova et al, 2017 ). BMP6 induces osteoblast differentiation ( Demirtaş et al, 2016 ) and bone mineralization ( Luo et al, 2011 ; Zhu et al, 2012 ). In addition to its role in bone resorption, TRAP acts as a clastokine by facilitating osteoblast proliferation and differentiation ( Gradin et al, 2012 ).…”

Section: Osteoclast Phenotype Functions and Biological Activitiesmentioning

confidence: 99%

Multinucleated Giant Cells: Current Insights in Phenotype, Biological Activities, and Mechanism of Formation

Ahmadzadeh

Vanoppen

Rosé

et al. 2022

Front. Cell Dev. Biol.

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Monocytes and macrophages are innate immune cells with diverse functions ranging from phagocytosis of microorganisms to forming a bridge with the adaptive immune system. A lesser-known attribute of macrophages is their ability to fuse with each other to form multinucleated giant cells. Based on their morphology and functional characteristics, there are in general three types of multinucleated giant cells including osteoclasts, foreign body giant cells and Langhans giant cells. Osteoclasts are bone resorbing cells and under physiological conditions they participate in bone remodeling. However, under pathological conditions such as rheumatoid arthritis and osteoporosis, osteoclasts are responsible for bone destruction and bone loss. Foreign body giant cells and Langhans giant cells appear only under pathological conditions. While foreign body giant cells are found in immune reactions against foreign material, including implants, Langhans giant cells are associated with granulomas in infectious and non-infectious diseases. The functionality and fusion mechanism of osteoclasts are being elucidated, however, our knowledge on the functions of foreign body giant cells and Langhans giant cells is limited. In this review, we describe and compare the phenotypic aspects, biological and functional activities of the three types of multinucleated giant cells. Furthermore, we provide an overview of the multinucleation process and highlight key molecules in the different phases of macrophage fusion.

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“…Recently, radiopaque beads (ROB) have been developed for embolization. Unlike traditional embolic microparticles, ROBs have iodine bound within their structure, which allows for real-time visualization of spatial distribution under standard clinical imaging [3, 5, 7-9]. This information has the potential to better define procedural endpoints, predict localized drug delivery and provide a higher level of patient specific treatment [3, 4, 7, 8, 10].…”

Section: Introductionmentioning

confidence: 99%

Distribution and Detection of Radiopaque Beads after Hepatic Transarterial Embolization in Swine: Cone-Beam CT versus MicroCT

Thompson

Sterren²,

Bakhutashvili

et al. 2018

Journal of Vascular and Interventional Radiology

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The full ROB distribution was more extensive than was apparent on cone-beam CT. Quantitative measures of embolic distribution demonstrated significantly better correlation with microCT with decreasing kVp. Similarly, qualitative analysis of MIPs showed improved visualization of beads with decreasing kVp. These findings demonstrate the clinical value of 80 kVp and 100 kVp protocols in the imaging of radiopaque embolizations compared with 120 kVp. However, considerations on X-ray penetration and dose may favor use of 100 kVp imaging over 80 kVp.

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Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation

Cited by 21 publications

References 31 publications

Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction

Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction

Multinucleated Giant Cells: Current Insights in Phenotype, Biological Activities, and Mechanism of Formation

Distribution and Detection of Radiopaque Beads after Hepatic Transarterial Embolization in Swine: Cone-Beam CT versus MicroCT

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