Effect of e-cigarette aerosol on gingival mucosa structure and proinflammatory cytokine response

Alanazi, Hanan; Rouabhia, Mahmoud

doi:10.1016/j.toxrep.2022.08.003

Cited by 5 publications

(1 citation statement)

References 37 publications

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“…11 A 4-week exposure to e-cigarettes also did not increase epithelial thickness or cell proliferation in rats. 13 Interestingly, short exposures to e-cigarette aerosols in engineered human oral 46 and nasal mucosa 47 reduces the number of proliferative cells. These findings suggest exposure to e-cigarettes and cigarettes may induce unique epithelial proliferative responses, and further studies on the larynx are needed.…”

Section: Discussionmentioning

confidence: 99%

Effects of Short‐term Electronic(e)‐Cigarette Aerosol Exposure in the Mouse Larynx

Easwaran,

Maria,

Martinez

et al. 2023

The Laryngoscope

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ObjectivesThe effects of electronic cigarettes (e‐cigarettes) on the larynx are relatively unknown. This study examined the short‐term effects of e‐cigarette inhalation on cellular and inflammatory responses within the mouse laryngeal glottic and subglottic regions after exposure to pod‐based devices (JUUL).MethodsMale C57BL6/J mice (8–9 weeks) were assigned to control (n = 9), JUUL flavors Mint (JMi; n = 10) or Mango (JMa; n = 10). JUUL mice were exposed to 2 h/day for 1, 5, and 10 days using the inExpose inhalation system. Control mice were in room air. Vocal fold (VF) epithelial thickness, cell proliferation, subglandular area and composition, inflammatory cell infiltration, and surface topography were evaluated in the harvested larynges. Mouse body weight and urinary nicotine biomarkers were also measured. Chemical analysis of JUUL aerosols was conducted using selective ion flow tube mass spectrometry.ResultsJUUL‐exposed mice had reduced body weight after day 5. Urinary nicotine biomarker levels indicated successful JUUL exposure and metabolism. Quantitative analysis of JUUL aerosol indicated that chemical constituents differ between JMi and JMa flavors. VF epithelial thickness, cellular proliferation, glandular area, and surface topography remained unchanged after JUUL exposures. Acidic mucus content increased after 1 day of JMi exposure. VF macrophage and T‐cell levels slightly increased after 10 days of JMi exposures.ConclusionsShort‐term e‐cigarette exposures cause minimal flavor‐ and region‐specific cellular and inflammatory changes in the mouse larynx. This work provides a foundation for long‐term studies to determine if these responses are altered with multiple e‐cigarette components and concentrations.Level of EvidenceN/A Laryngoscope, 2023

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