Effect of cannabis smoke condensate on human nasal epithelial cell adhesion, growth, and migration

Rouabhia, Mahmoud; Piché, Marie-Ève; Hazzi, Christina; Corriveau, Marie-Noëlle; Chakir, Jamila

doi:10.1016/j.amjoto.2023.103890

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…012 ™ ) were seeded (2 × 10^5) onto each type (PVA, PVA-PG, PVA-VG, or PVA-PG-VG) of hydrogel and cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum, 100 U/mL penicillin G, 25 μg/mL streptomycin, and 0.5 μg/mL fungizone at 37 °C in a 5% CO 2 humid atmosphere for 24 h. At this time, the culture of each hydrogel was collected and centrifuged; the supernatant was aliquoted in three tubes and frozen at −80 °C. The cells adhering to each hydrogel were fed fresh medium containing 10% of methyl thiazolyl tetrazolium salt (MTS) reagent (ab197010, Abcam, Cambridge, United Kingdom), and incubated for 3 h at 37 °C in a dark atmosphere (Rouabhia et al, 2023). At the end of the incubation time, 200 μL, in triplicate, was placed in wells of a 96-well plate, and the absorbance was read at 499 nm using an X-Mark microplate spectrophotometer (Bio-Rad, Mississauga).…”

Section: Ftir Characterizationmentioning

confidence: 99%

Release of delta-9-tetrahydrocannabinol from polyvinyl alcohol hydrogels and its safe interaction with human skin fibroblasts

Cui,

Zhang,

Rodrigue

et al. 2024

Front. Drug Deliv.

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This study aimed to design a THC-rich hydrogel to deliver cannabis derivatives topically. We developed hydrogels using polyvinyl alcohol (PVA) mixed with propylene glycol (PG), vegetable glycerin (VG), or both to facilitate the dissolution of delta-9-tetrahydrocannabinol (THC). The hydrogels showed a brown color, confirming the presence of the cannabinoid. They exhibit a porous structure and better mechanical properties than PVA alone. Indeed, the hydrogel containing PG, VG, or both showed elastic deformation behaviors with lower water content. FTIR analysis demonstrated the presence of THC with two specific peaks at 1,575 and 1,619 cm−1, confirming the presence of THC in the hydrogels. Human dermal fibroblast cultures onto the surface of all hydrogels confirmed the safety of the THC-rich hydrogel as the cell adhesion was comparable to the control (no THC). Furthermore, cells adhering to the hydrogels could proliferate, showing increased cell viability at 48 and 72 h, with a higher proliferation obtained with the THC-rich PVA-PG-VG hydrogels. Such cell behavior could be due to the release of the THC in the culture medium, as demonstrated by ultra-high performance liquid chromatography (UPLC), showing the presence of THC in the culture medium, ranging from 203 to 290 μg after 24 h of incubation of the hydrogels containing PG and VG or both. In comparison, the released THC from the PVA hydrogel was higher, reaching 852 μg. It is interesting to note that the THC release at 24, 48, and 72 h was slower with the hydrogels containing PG, VG, and both, compared to PVA alone. Overall, the present study has designed safe THC-rich PVA-PG-VG hydrogels as a functional delivery system for the topical use of cannabinoids to control tissue diseases, such as inflammation.

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Section: Ftir Characterizationmentioning

confidence: 99%