Euparen when applied to the soil at three doses (0.95, 4.75 and 9.5 mg active ingredient N,N-dimethyl-N'-phenyl-(N'-fluorodichloro-methy1thio)-sulfamide per kg dry soil) was generally suppressive to the total count of fungi and several fungal species. its toxicity persisted to act till after 10 weeks from treatment with the three doses, but after 15 weeks the toxicity was completely alleviated. Trichoderma spp. were the only fungal species that was recovered from soil when Euparen was incorporated into cellulose-CzApEKs agar medium at the three doses (4.8, 23.8 and 47.5 ppm). The decomposition of filter paper buried in Euparen-treated soil was significantly retarded after 1 week of incubation with the high dose. On the other hand, filter paper sprayed with the fungicide at the high dose and buried in untreated soil was decomposed at a significantly lower rate than the control after all experimental periods (15 weeks) in addition to the medium dose after 5 weeks of incubation. The growth and sporulation of Trichoderma viride on filter papers placed on the surface of carbon-free agar medium and treated with Euparen were partially increased by the low dose, not affected by the medium dose and partially inhibited by the high dose. The colonization of the other test fungi was partially inhibited or even completely eliminated by the three doses. The mycelial dry weight of Trichoderma viride was significantly increased (nearly doubled) by the medium dose and not affected by the other two doses. The mycelial dry weight and endo 1,4-b-~-glucanase production of the other test fungi were toxicated at least by the higher doses.Euparen (Dichlofluanid ; is a fungicide manufactured by BAYER Pflanzenschutz, West Germany with a chemical name N,N-dimethyl-N'-phenyl-(N'-fluorodichloro-methylthio)-sulfamide and is used in Egypt for the control of graps, strawberries, deciduous fruits and roses diseases.This investigation aimed at studying the side effect of Euparen on the population of cellulose-decomposing fungi and the decay of cellulose in soil. In addition, the effect of this fungicide on the growth and sporulation, the mycelial dry weight and endo-1,4P-~-glucanase production of eleven common cellulose-decomposing fungal species were tested in vitro.
Materials and methodsApplication of Euparen to the soil: Sandy-clay soil aliquot collected from Assiut area, Egypt, with pH 8.1, 0.82 % organic matter content, and 0.12% total soluble salts was used. 500 g of air-dry sieved soil (sieve, 20) were placed in a polyethylene bag which was maintained in a plastic pot. Euparen was added and thoroughly mixed with the soil in three doses: low, equivalent to the recommended field dose (0.95 mg active ingredient/ kg dry soil), medium, equivalent to five-times the field dose (4.75 mg), and high, equivalent to ten-times the field dose (9.5 mg). The water content of the soil was adjusted to 40.0 % of its maximum water holdingcapacity, using HILGARD'S pan. Treatments were set up in duplicate in addition to the control without Euparen. Pots were...