Effect of different calcium phosphate scaffold ratios on odontogenic differentiation of human dental pulp cells

AbdulQader, Sarah Talib; Kannan, Thirumulu Ponnuraj; Rahman, Ismail Ab; Ismail, Hanafi; Mahmood, Zuliani

doi:10.1016/j.msec.2014.12.070

Cited by 26 publications

(23 citation statements)

References 65 publications

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“…An ideal scaffold with osteoconductivity should have macropores that are 150-500 µm with 60-80% interconnected porosity. 59 We previously confirmed that prepared scaffolds exhibited interconnected macropores that were 150-200 µm in diameter, with an average porosity of 95-98%, which could provide an appropriate environment for inducing osteogenic differentiation. The obtained interconnectivity of the macropores is significant for tissue ingrowth into the material.…”

Section: Resultsmentioning

confidence: 68%

Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects

Tebyanian

Norahan

Eyni

et al. 2019

Journal of Applied Biomaterials & Functional Materials

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Bone defects remain a significant health issue and a major cause of morbidity in elderly patients. Composites based on collagen/calcium phosphate have been widely used for bone repair in clinical applications, owing to their comparability to bone extracellular matrix. This study aimed to evaluate the effects of a scaffold of collagen/calcium phosphate (COL/β-TCP) on bone formation to assess its potential use as a bone substitute to repair bone defects. Bilateral full-thickness critically sized calvarial defects (8 mm in diameter) were created in New Zealand white rabbits and treated with COL/β-TCP or COL scaffolds. One defect was also left unfilled as a control. Bone regeneration was assessed through histological evaluation using hematoxylin and eosin and Masson's trichrome staining after 4 and 8 weeks. Alizarin Red staining was also utilized to observe the mineralization process. Our findings indicated that COL/β-TCP implantation could better enhance bone regeneration than COL and exhibited both new bone growth and scaffold material degradation.

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Section: Resultsmentioning

confidence: 68%

Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects

Tebyanian

Norahan

Eyni

et al. 2019

Journal of Applied Biomaterials & Functional Materials

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“…apatite-forming ability) and biodegradability, suitable porous structure [8] and ability to support osteo/odontogenic differentiation and biomineralization [9]. Tailoring ionic dissolution (= biointeractivity) from bioceramic materials is key to achieving specific biological responses, since many elements released by these materials, such as Si 4+ , P, Zn 2+ , Mg 2+ , or Ca 2+ ions, participate in signaling pathways and intracellular interactions in the human body [10].…”

Section: Introductionmentioning

confidence: 99%

Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

et al. 2016

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“…Dental pulp cells (DPCs) are clonal precursors of dental pulp tissue, possessing self-renewal and multilineage differentiation capability. DPCs could differentiate into odontoblasts, adipocytes, chondrocytes and neurons, and so forth [ 1 ]. Lipopolysaccharide (LPS), one of the central regulators of pulpal pathogenesis, can bind to Toll-like receptor 4 (TLR4), to initiate inflammatory cascade through mediating synthesis and secretion of various inflammatory cytokines, causing damage or even necrosis of dental pulp [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells

Liu

Shan

Cheung

et al. 2016

BioMed Research International

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Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.

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Effect of different calcium phosphate scaffold ratios on odontogenic differentiation of human dental pulp cells

Cited by 26 publications

References 65 publications

Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects

Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects

Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells

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