Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells

Abstract

Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.

Decreased expression of microRNA‑30b promotes the development of pulpitis by upregulating the expression of interleukin‑6 receptor

“…MicroRNAs (miRNAs or miRs) are a type of non-coding, small RNA molecule made up of 18-22 nucleotides, which regulate the expression of proteins at mRNA level in eukaryotes (11)(12)(13). The pathogenesis of pulpitis is accompanied by changes in the expression of a variety of miRNA molecules and proteins, suggesting that miRNA may serve important roles in the regulation of proteins associated with the disease (14)(15)(16). In the present study, the expression of IL-6R mRNA and protein were examined in pulp tissues, blood and saliva from patients with pulpitis.…”

Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells

“…In pheochromocytoma PC12 cells, BPS can regulate the expression of miR-10b to inhibit the expression of KLF4 and induce cell migration (Jia et al, 2018). It has been reported that miR-15a (Zhu et al, 2017), miR-146a (Liu et al, 2016), and miR-195 (Wang et al, 2017) can also regulate the mRNA stability and expression of bFGF.…”

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

“…[60] Polyethyleneimine (PEI) derivatives have also been extensively studied as miRNA delivery systems, [19,61,62] and specifically PEI-poly(ethylene glycol) (PEG) nanoparticles (NP) have recently demonstrated interesting profiles when encapsulated within poly(lactic-co-glycolic acid) (PLGA) microspheres in a poly(l-lactic acid) (PLLA) scaffold [63] or as part of a calcium chloride cross-linked alginate hydrogel system. [64] PEI is watersoluble and easily attracts negatively charged ions to aid the polyplex cargo escape the endolysosomal compartment in what is known as "the proton sponge effect." [65] The extent of this effect is dependent on a parameter termed N/P ratio, that is, the proportion of moles of nitrogen in PEI to moles of phosphate in the cargo.…”