Effect of Diethylstilbestrol, Ascorbic Acid and Vitamin E on Serum Lipid Patterns

Clegg, R. E.; Klopfenstein, C. F.; Klopfenstein, W. E.

doi:10.3382/ps.0551104

Cited by 12 publications

(11 citation statements)

References 7 publications

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“…However, adding vitamin E significantly lowered the serum cholesterol from that when Wesson Oil-cholesterol alone or with ascorbic acid was fed. A similar result was observed in DES-induced hyperlipemia (Clegg et al, 1976). In both cases serum cholesterol rose less in the vitamin E group than in the control group.…”

Section: Resultssupporting

confidence: 81%

“…DES with vitamin E left triglycerides higher than ascorbic acid and vitamin E did. In this study phospholipids did not differ significantly among groups, but with D Estreated birds (Clegg et al, 1976) the phospho-lipids were higher.…”

Section: Resultscontrasting

confidence: 44%

“…Types and amounts of lipids in the serum were determined by TLC and the fatty acids by GLC as described by Clegg et al (1976).…”

Section: Methodsmentioning

confidence: 99%

“…Spittle (1971Spittle ( , 1974 and Ginter et al (1969) have linked serum lipid variations to high ascorbic acid supplements in humans and guinea pigs. Clegg et al (1976) have reported effects of ascorbic acid and vitamin E on lipids in blood serum of cockerels under high DES stress. Reported here are effects of ascorbic acid and vitamin E on the serum lipids and the fatty acid distribution in the blood serum of cockerels fed a Wesson Oil-cholesterol supplement.…”

mentioning

confidence: 97%

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Effects of Ascorbic Acid and Vitamin E on Serum Lipids of Cockerels Fed Wesson Oil-cholesterol Diets

Klopfenstein

Clegg

1977

Poultry Science

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The major lipid changes caused by adding 5% Wesson Oil and 1% cholesterol to cockerel diets were large increases in serum cholesterol, cholesteryl esters, and triglycerides. Lecithin and cephalin were not affected. Ascorbic acid significantly increased serum triglycerides; vitamin E significantly lowered serum cholesterol. Percentages of the saturated acids (palmitic, stearic, and arachidonic) of cockerels on the Wesson Oil-cholesterol diet decreased significantly; unsaturated linolenic and linolenic acids increased, and oleic acid remained the same. Dietary ascorbic acid seemed not to affect fatty acid distribution significantly, but when vitamin E was added to the diet, the stearic acid percentage rose and oleic acid percentage was lower. Acrylamide-gel electrophoresis showed that changes the vitamin E caused in the lipid and protein constituents of cockerel serum differed from changes the Wesson Oil-cholesterol diet caused.

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Section: Resultssupporting

confidence: 81%

Section: Resultscontrasting

confidence: 44%

“…Types and amounts of lipids in the serum were determined by TLC and the fatty acids by GLC as described by Clegg et al (1976).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 97%

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Effects of Ascorbic Acid and Vitamin E on Serum Lipids of Cockerels Fed Wesson Oil-cholesterol Diets

Klopfenstein

Clegg

1977

Poultry Science

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“…In contrast, the rise in serum phospholipid and triglyceride contents produced by vitamin E in chicks treated with diethylstilbesterol was reversed by the co-administration of vitamin C [32]. Although the mechanism of interaction in this instance is not known, it appears that mutual influences of these vitamins can occur in all tissues.…”

Section: Discussionmentioning

confidence: 59%

Effect of vitamins C and E on sulfated proteoglycan metabolism and sulfatase and phosphatase activities in organ cultures of human cartilage

Schwartz

1979

Calcif Tissue Int

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Summary.Previous studies conducted in cell culture utilizing chondrocytes isolated from human articular cartilage showed that inclusion of ascorbic acid in the nutrient medium resulted in an increase in the net biosynthesis of sulfated proteoglycans concomitant with a reduction in the activities of arylsulfatases A and B. This suggested a possible connection between the stability of the sulfated macromolecules and the activity of these desulfating enzymes. To examine whether this relationship also was operative in a more native milieu, the effect of ascorbate on these parameters was measured in an organ culture system using slices of human articular cartilage where the structural extracellular framework surrounding the chondrocytes is comparable to that present in vivo. In addition, since numerous studies have shown that vitamin E inhibits lysosomal enzymes, the effect of alpha-tocopherol on these parameters was also investigated.Concurrent with an inhibition of arylsulfatase A and B activities, an increase in sulfated proteoglycan biosynthesis per unit of DNA as reflected in 35S-sulfate uptake was present in all cartilage specimens examined 6 days after the introduction of vitamin C in the culture fluid. A larger fraction of the newly synthesized sulfated macromolecules was incorporated into the matrix of the tissue in samples maintained in the vitamin-supplemented cultures.Supplementation of the organ culture medium with vitamin E resulted in the inhibition of arylsulfatase A and no change in arylsulfatase B activities. In contrast to the action of vitamin C which stimulated acid phosphatase activity, a potent inhibition of this enzyme was effected by vitamin E.Sulfated proteoglycan content was greatly enhanced by alpha-tocopherol, although the vitamin Send offprint requests to Dr. Edith R. Schwartz at the above address.did not alter the distribution of the newly synthesized molecules between the tissue and medium fractions.Inhibition of acid phosphatase and arylsulfatase A by alpha-tocopherol was evident in tissue lysates and partially purified enzyme preparations. This implies a direct interaction between the enzymes and vitamin E.The actions of alpha-tocopherol and ascorbate on cartilage enzymes and structural components were dissimilar. Both vitamins, however, appeared to enhance the stability of sulfated proteoglycans in the complex structure comprising articular cartilage.

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Comparative plasma lipid response of pullets and laying hens to estradiol and progesterone

Hagan

Leszczynski

Kummerow

1984

Toxicology and Applied Pharmacology

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Effect of Diethylstilbestrol, Ascorbic Acid and Vitamin E on Serum Lipid Patterns

Cited by 12 publications

References 7 publications

Effects of Ascorbic Acid and Vitamin E on Serum Lipids of Cockerels Fed Wesson Oil-cholesterol Diets

Effects of Ascorbic Acid and Vitamin E on Serum Lipids of Cockerels Fed Wesson Oil-cholesterol Diets

Effect of vitamins C and E on sulfated proteoglycan metabolism and sulfatase and phosphatase activities in organ cultures of human cartilage

Comparative plasma lipid response of pullets and laying hens to estradiol and progesterone

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