Effect of cytokinin on alkaloid accumulation in periwinkle callus cultures transformed with a light-inducible ipt gene

Garnier, Frédérique; Carpin, Sabine; Label, Philippe; Crèche, Joël; Rideau, Marc; Hamdi, Saı̈d

doi:10.1016/s0168-9452(96)04485-8

Cited by 25 publications

(4 citation statements)

References 28 publications

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“…The use of 2,4-D decreased the accumulation of these metabolites. Garnier et al (1996) found that BAP (6-benzylaminopurine) and in some cases NAA (naphtahleneacetic acid) stimulated the accumulation of vindoline and catharanthine in periwinkle.…”

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Wesołowska

Grzeszczuk

Wilas

et al. 2016

Not Bot Horti Agrobo

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Catharanthus roseus (periwinkle) is a medicinal plant commonly known for its wide biological activity. In many countries different parts of this plant are used for the treatment of diabetes, hypertension and for menstrual regulation. Due to the ability of production of alkaloids, which can be applied in cancer therapy, is still extensively investigated. Two, the most valuable alkaloids (vincristine and vinblastine) are present in C. roseus in very low concentrations. Micropropagation is promising technique used to enhance the level of important secondary metabolites. The main objective of present study was alkaloids extraction from plants cultivated conventionally and derived from in vitro cultures. In this order the aerial parts of periwinkle were extracted with 96% ethanol at room temperature (method I) and heated with 96% ethanol at 55 °C for 90 minutes (method II). The obtained mixtures of different indole alkaloids were analyzed by gas chromatography -mass spectrometry (GC-MS). Analysis revealed the presence of 15 alkaloids, among which vindoline, vindorosine, isovindolinine and ajmalicine were the most abundant. The obtained results indicated that the propagation method had a significant effect on the percentage content of alkaloids in C. roseus herb. Plants derived from in vitro cultures were richer in vindorosine and vindoline, while conventionally cultivated -in tetrahydroalstonine and ajmalicine. Moreover, in case of isovindolinine, vindolinine and ajmalicine, extraction at 55 °C was more effective, while for pericyclivine -maceration at room temperature. Interestingly, the pericyclivine was not detected in the mixture of alkaloids obtained from periwinkle herb by the extraction at 55 °C.

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Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Wesołowska

Grzeszczuk

Wilas

et al. 2016

Not Bot Horti Agrobo

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“…The callus derived from stem explant showed significantly higher levels of CBA as compared to root, cotyledon and leaf derived callus on BA enriched medium (Table 4). Cytokinins have been demonstrated to stimulate accumulation of alkaloids in cultures of periwinkle (Garnier et al 1996), Nothapodytes foetida (Thengane et al 2003), and Stephania tetrandra (Kuo et al 2011). Contrasting to these, cytokinins were inhibitory for naphthoquinone formation in Lithospermum erythrorhizon (Fujita et al 1981).…”

Section: Boswellic Acid Production In Callusmentioning

confidence: 99%

Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb.

Nikam

Ghorpade

Nitnaware

et al. 2012

Physiol Mol Biol Plants

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Micropropagation through cotyledonary and leaf node and boswellic acid production in stem callus of a woody medicinal endangered tree species Boswellia serrata Roxb. is reported. The response for shoots, roots and callus formation were varied in cotyledonary and leafy nodal explants from in vitro germinated seeds, if inoculated on Murshige and Skoog's (MS) medium fortified with cytokinins and auxins alone or together. A maximum of 8.0 ± 0.1 shoots/cotyledonary node explant and 6.9 ± 0.1 shoots/leafy node explants were produced in 91 and 88 % cultures respectively on medium with 2.5 μM 6-benzyladenine (BA) and 200 mg l(-1) polyvinylpyrrolidone (PVP). Shoots treated with 2.5 μM IBA showed the highest average root number (4.5) and the highest percentage of rooting (89 %). Well rooted plantlets were acclimatized and 76.5 % of the plantlets showed survival upon transfer to field conditions. Randomly amplified polymorphic DNA (RAPD) analysis of the micropropagated plants compared with mother plant revealed true-to-type nature. The four major boswellic acid components in calluses raised from root, stem, cotyledon and leaf explants were analyzed using HPLC. The total content of four boswellic acid components was higher in stem callus obtained on MS with 15.0 μM IAA, 5.0 μM BA and 200 mg l(-1) PVP. The protocol reported can be used for conservation and exploitation of in vitro production of medicinally important non-steroidal anti-inflammatory metabolites of B. serrata.

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“…Zeatin enhanced the activity of G10H and the bioconversion of secologanin to ajmalicine in C. roseus cultures (Decendit et al 1993). Exogenously applied cytokinins to untransformed C. roseus callus or cell suspension cultures increased the content of ajmalicine and serpentine (Garnier et al 1996;Yahia et al 1998). Abscisic acid (ABA) regulates various aspects of plant growth and development including seed maturation and dormancy, as well as adaptation to abiotic environmental stresses (Davies and Jones 1991;Beaudoin et al 2000).…”

Section: Plant Growth Regulatorsmentioning

confidence: 99%

Catharanthus terpenoid indole alkaloids: biosynthesis and regulation

2007

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Catharanthus roseus is still the only source for the powerful antitumour drugs vinblastine and vincristine. Some other pharmaceutical compounds from this plant, ajmalicine and serpentine are also of economical importance. Although C. roseus has been studied extensively and was subject of numerous publications, a full characterization of its alkaloid pathway is not yet achieved. Here we review some of the recent work done on this plant. Most of the work focussed on early steps of the pathway, particularly the discovery of the 2-C-methyl-D-erythritol 4-phosphate (MEP)-pathway leading to terpenoids. Both mevalonate and MEP pathways are utilized by plants with apparent cross-talk between them across different compartments. Many genes of the early steps in Catharanthus alkaloid pathway have been cloned and overexpressed to improve the biosynthesis. Research on the late steps in the pathway resulted in cloning of several genes. Enzymes and genes involved in indole alkaloid biosynthesis and various aspects of their localization and regulation are discussed. Much progress has been made at alkaloid regulatory level. Feeding precursors, growth regulators treatments and metabolic engineering are good tools to increase productivity of terpenoid indole alkaloids. But still our knowledge of the late steps in the Catharanthus alkaloid pathway and the genes involved is limited.

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Effect of cytokinin on alkaloid accumulation in periwinkle callus cultures transformed with a light-inducible ipt gene

Cited by 25 publications

References 28 publications

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb.

Catharanthus terpenoid indole alkaloids: biosynthesis and regulation

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