Effect of cultivation conditions on the adhesive activity of Rhodococcus cells towards n-Hexadecane

Rubtsova, E. V.; Kuyukina, Maria S.; Ившина, И. Б.

doi:10.1134/s0003683812050110

Cited by 16 publications

(10 citation statements)

References 14 publications

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“…Treatment with increasing concentrations of Ni NPs did not significantly affect the degree of hydrophobicity of rhodococcal cells. The data obtained are consistent with previous studies [ 11 , 16 ] confirming the hydrophobic nature of Rhodococcus cells as their intrinsic adaptation to the assimilation of hydrocarbon substrates.…”

Section: Resultssupporting

confidence: 92%

“…Bacteria grown in nutrient broth (28 °C, 3 days) were harvested, washed twice and suspended in ddH 2 O or 10 mM KNO 3 to an optical density (OD 600 ) of 0.5. The relative hydrophobicity of bacterial cells was determined in salt aggregation test (SAT) using ammonium sulfate in increasing concentrations of 0.2, 0.6 and 1.0 M [ 16 ]. Ten microliters of bacterial suspension in ddH 2 O was gently mixed with 10 μL of (NH 4 ) 2 SO 4 on the microscope slide.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties

2022

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Nickel nanoparticles (NPs) are used for soil remediation and wastewater treatment due to their high adsorption capacity against complex organic pollutants. However, despite the growing use of nickel NPs, their toxicological towards environmental bacteria have not been sufficiently studied. Actinobacteria of the genus Rhodococcus are valuable bioremediation agents degrading a range of harmful and recalcitrant chemicals. Both positive and negative effects of metal ions and NPs on the biodegradation of organic pollutants by Rhodococcus were revealed, however, the mechanisms of such interactions, in addition to direct toxic effects, remain unclear. In the present work, the influence of nickel NPs on the viability, surface topology and nanomechanical properties of Rhodococcus cells have been studied. Bacterial adaptations to high (up to 1.0 g/L) concentrations of nickel NPs during prolonged (24 and 48 h) exposure were detected using combined confocal laser scanning and atomic force microscopy. Incubation with nickel NPs resulted in a 1.25–1.5-fold increase in the relative surface area and roughness, changes in cellular charge and adhesion characteristics, as well as a 2–8-fold decrease in the Young’s modulus of Rhodococcus ruber IEGM 231 cells. Presumably, the treatment of rhodococcal cells with sublethal concentrations (0.01–0.1 g/L) of nickel NPs facilitates the colonization of surfaces, which is important in the production of immobilized biocatalysts based on whole bacterial cells adsorbed on solid carriers. Based on the data obtained, cell surface functionalizing with NPs is possible to enhance adhesive and catalytic properties of bacteria suitable for environmental applications.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties

2022

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“…Exposed to ecotoxicants, rhodococci can vary the fatty acid composition of membrane lipids, including mycolic acids and phospholipids, to maintain the level of viscosity of their membranes in the liquid-crystalline state [ 108 , 115 ]. Lipids are one of the few groups of complex organic substances for which the composition can be adequately regulated by the bacterial cell in response to suboptimal growth conditions by changing the structure and relative amount of membrane fatty acids [ 116 ].…”

Section: Adaptive Cell Modifications Of Rhodococci Exposed To Hydrocarbons and Other Environmental Pollutantsmentioning

confidence: 99%

“…The total lipid content and composition in the cell wall play a key role in the sorption of liquid hydrocarbons [ 135 , 136 ]. For example, in R. ruber (IEGM 324, IEGM 333, and IEGM 371 strains) grown in a mineral salt medium supplemented with n -hexadecane, approximately a two-fold increase in the number of total lipids (17–22% of the dry cell weight) was found compared with that (8–14%) of cells grown in the nutrient broth [ 115 ]. Furthermore, an increase in straight chain saturated fatty acids and neutral phospholipids (particularly cardiolipin and phosphatidylethanolamine) was observed, which apparently leads to an increase in the degree of cellular adhesion to nonpolar hydrocarbons [ 111 , 115 , 122 , 137 ].…”

Section: Adaptive Cell Modifications Of Rhodococci Exposed To Hydrocarbons and Other Environmental Pollutantsmentioning

confidence: 99%

Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species

et al. 2021

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Under conditions of increasing environmental pollution, true saprophytes are capable of changing their survival strategies and demonstrating certain pathogenicity factors. Actinobacteria of the genus Rhodococcus, typical soil and aquatic biotope inhabitants, are characterized by high ecological plasticity and a wide range of oxidized organic substrates, including hydrocarbons and their derivatives. Their cell adaptations, such as the ability of adhering and colonizing surfaces, a complex life cycle, formation of resting cells and capsule-like structures, diauxotrophy, and a rigid cell wall, developed against the negative effects of anthropogenic pollutants are discussed and the risks of possible pathogenization of free-living saprotrophic Rhodococcus species are proposed. Due to universal adaptation features, Rhodococcus species are among the candidates, if further anthropogenic pressure increases, to move into the group of potentially pathogenic organisms with “unprofessional” parasitism, and to join an expanding list of infectious agents as facultative or occasional parasites.

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“…This result was expected, when one considers the low availability of mineral nutrients in the seawater used in this study. Both bacterial species used in the microcosms seem to have mechanisms to modify the hydrophobicity of cell surface to possible adhesion to hydrocarbons and then facilitate the catabolism of these molecules [57,58]. This fact can be related to the increase of CO 2 emission from inoculated treatments.…”

Section: Effect Of W/o/w Emulsions On Oil Biodegradationmentioning

confidence: 99%

Strategy to improve crude oil biodegradation in oligotrophic aquatic environments: W/O/W fertilized emulsions and hydrocarbonoclastic bacteria

et al. 2020

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We studied petroleum biodegradation by biostimulation by using water in oil in water (W/O/W) double emulsions. These emulsions were developed using seawater, canola oil, surfactants, and mineral salts as sources of NPK. The emulsions were used in the simulation of hydrocarbon bioremediation in oligotrophic sea water. Hydrocarbon biodegradation was evaluated by CO 2 emissions from microcosms. We also evaluated the release of inorganic nutrients and the stability of the emulsion's droplets. The double emulsions improved CO 2 emission from the microcosms, suggesting the increase in the hydrocarbon biodegradation. Mineral nutrients were gradually released from the emulsions supporting the hydrocarbon biodegradation. This was attributed to the formation of different diameters of droplets and therefore, varying stabilities of the droplets. Addition of the selected hydrocarbonoclastic isolates simulating bioaugmentation improved the hydrocarbon biodegradation. We conclude that the nutrient-rich W/O/W emulsion developed in this study is an effective biostimulation agent for bioremediation in oligotrophic aquatic environments.

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Effect of cultivation conditions on the adhesive activity of Rhodococcus cells towards n-Hexadecane

Cited by 16 publications

References 14 publications

Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties

Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties

Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species

Strategy to improve crude oil biodegradation in oligotrophic aquatic environments: W/O/W fertilized emulsions and hydrocarbonoclastic bacteria

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