Effect of crocin on aged rat kidney through inhibition of oxidative stress and proinflammatory state

Abstract

This study evaluated whether crocin, a bioactive component of saffron, has a protective effect on kidney through reducing the oxidative stress and inflammatory response in aged rats. In this study the changes in activities of antioxidant enzymes, lipid peroxidation, glutathione (GSH) levels and the expression of pro-inflammatory cytokines in the serum and renal tissue were evaluated by ELISA and RT-PCR, respectively. The middle and aged rats were given intraperitoneal injections of crocin (10, 20, 30 mg/kg/day) for 4 weeks. After 4 weeks, animals were anesthetized with diethyl ether. The kidney samples were taken for biochemical analysis. The results revealed the aging was associated with a significant decrease in the activities of antioxidant enzymes, and GSH content with increase in lipid peroxidation level in kidney of the aged rats (p < 0.001). The increased levels of serum renal functional parameter, oxidative parameters (p < 0.01) and also pro-inflammatory cytokine levels were significantly reduced by crocin administration (p < 0.05). The aged rats exhibited a dysregulation of the oxidative stress, and inflammation in the kidneys, but crocin treatment significantly reduced the expression of the inflammatory genes. These results provide pivotal documentation that crocin has a renoprotective effects against the development of oxidative stress and inflammation in the kidney of old rats. Copyright © 2016 John Wiley & Sons, Ltd.

Protective effect of crocin on ultraviolet B‑induced dermal fibroblast photoaging

“…Crocin is one of the main and active constituents isolated from saffron. It has been shown previously that crocin exhibits multiple activities, including anti-cancer, anti-inflammatory and anti-oxidation effects in various cell types ( 7 ). Lv et al ( 8 ) reported that crocin exerts an anti-oxidative effect against H 2 O 2 -induced apoptosis in retinal ganglion cells.…”

Untitled

“…In such cases, therefore, certain therapies such as surgery, chemical pharmacotherapy, and psychiatric treatments can be useful 61,62 . It is obvious that medicinal herbs and their products via antioxidant activities and anti inflammatory properties can be benefit for improvement a lot of diseases [63][64][65][66][67][68][69][70][71][72][73][74][75] .…”

Agents-induced nephrotoxicity and catcheins

“…Due to kidneys receive many vessels, it may concentrate various drugs and toxic chemicals within the renal cortex and medulla rejoins [2]. Almost all of the nephrotoxic agents bind to the brush border receptors of the renal proximal convoluted tubules and concentrate within proximal tubular cells [3]. Imbalance between oxidantantioxidant system is the main mechanism of renal injury [3].…”

“…Almost all of the nephrotoxic agents bind to the brush border receptors of the renal proximal convoluted tubules and concentrate within proximal tubular cells [3]. Imbalance between oxidantantioxidant system is the main mechanism of renal injury [3]. Herbs rich in flavonoids have been detected as an important therapy for renal failures because of their antioxidant and antiinflammatory effects [4][5][6].…”