Protective effect of crocin on ultraviolet B‑induced dermal fibroblast photoaging

Abstract

Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS), resulting in the aging of dermal fibroblasts. Crocin, a bioactive constituent of Crocus sativus, possesses anti-oxidation effects. The purpose of the present study was to evaluate the protective effect of crocin on UVB-induced dermal fibroblast photoaging. Human dermal fibroblasts were isolated and cultured with different concentrations of crocin prior to and following exposure to UVB irradiation. The senescent phenotypes of cells were evaluated, including cell proliferation, cell cycle, senescence-associated β-galactosidase (SA-β-gal) expression, intracellular ROS, expression of antioxidant protein glutathione peroxidase 1 (GPX-1) and extracellular matrix protein collagen type 1 (Col-1). Crocin rescued the cell proliferation inhibited by UVB irradiation, prevented cell cycle arrest and markedly decreased the number of SA-β-gal-positive cells. In addition, crocin reduced UVB-induced ROS by increasing GPX-1 expression and other direct neutralization effects. Furthermore, crocin promoted the expression of the extracellular matrix protein Col-1. Crocin could effectively prevent UVB-induced cell damage via the reduction of intracellular ROS; thus, it could potentially be used in the prevention of skin photoaging.

Extracellular vesicles from adipose-derived stem cells ameliorate ultraviolet B-induced skin photoaging by attenuating reactive oxygen species production and inflammation

“…Human fibroblasts were cultured in 96-well (2000 cells/ well) or 6-well plates (1 × 10 5 cells/well) in a culture medium, supplemented with or without different doses of EVs, at 37°C for 24 h. After the supernatant was removed, cells were washed with PBS twice and covered by a thin layer of PBS. Then, cells were exposed to UVB light (Philip, 311 nm, 20 W/01, Germany) at a total dose of 100 mJ/cm 2 as previously described [22]. After irradiation, PBS was removed and replaced with culture medium for 24 or 72 h for further experiments.…”

“…ROS accumulation has been reported to induce cell cycle arrest [22,35]. After UVB irradiation, the S and G2 phases of the FB cell cycle were arrested (Fig.…”

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“…Though UVB is absorbed in the epidermis and has little effect on the dermis [40], such irradiation induces ROS generation of HDF cells in vitro [41,42]. Since dermal fibroblasts are the primary cells secreting MMPs and elastase [32,36], we evaluated the biological effect of UVB-induced oxidative response in HDF cells.…”

Anti-aging Effects of Select Botanicals: Scientific Evidence and Current Trends

“…It also decreases the expression of multiple NF-κB dependent genes [45]. In a study using cultured human fibroblasts, crocin reduced UV-induced ROS, promoted expression of extracellular matrix protein Col-1, and decreased the number of cells with senescent phenotypes after UV radiation [47]. It decreases ROS production and limits apoptosis [46].…”