Effect of commercially available nano-hydroxy apatite containing desensitizing mouthwash on dentinal tubular occlusion: an in vitro FESEM analysis

Abstract

Background and aim. Dentinal hypersensitivity is a significant clinical problem encountered in daily dental practice. The management of this condition requires a good understanding of the complexity of the problem, as well as the variety of treatments currently available. The treatment approaches can be either home care products or professionally applied desensitizing agents. The present in-vitro study was designed to investigate the dentinal tubule occluding ability of commercially available nano HA containing mouthwash using FESEM analysis.
 Methods: In the present in -vitro study, 15 human premolars and canines were taken and sectioned mesiodistally. A total of 30 dentinal samples were obtained. All the dentinal discs were etched with 6% citric acid for 2 minutes. The treated samples were washed thoroughly with distilled water for 30 seconds. Samples were divided in two groups of 15 each. The specimens in Group I were shaken vigorously in the Vitis Sensitive mouthwash for 2 min twice daily for 14 days. After this intervention samples were placed in distilled water. Group II were immersed in distilled water. Samples were subjected to FESEM to analyze for tubular occlusion.
 Results. In group I nearly complete dentinal surface occlusion was present on the 7th and 14th day and precipitates were seen covering a large part of the dentinal surface. In group II no dentinal tubular occlusion was observed.
 Conclusion. The results of the present study support the ability of nHA containing Vitis sensitive mouthwash to occlude the dentinal tubules and thus it may demonstrate a significant reduction in dentinal hypersensitivity when used clinically.

Evaluation The Efficiency of Nano Seashell, Sodium Fluoride And A Commercially Available Toothpaste On Dentinal Tubules Occlusion After Acid Attack Using Scanning Electron Microscope: In vitro Study.

“…For group 1(No acid challenge); subgroup A received no treatment, subgroups B, C and D were treated with Sodium fluoride (NaF) paste, Nano seashell paste and signal Complete 8 paste respectively. The paste was rubbed on the sample surface using a micro brush for 2 minutes after which they were rinsed with deionized water for 30 seconds and then stored in the container filled with artificial saliva at 37 ֯ C. [29] This treatment procedure was repeated every 12 hours for 14 days [30,31] . The artificial saliva was changed every 24 hours.…”

Efficacy of Hydroxyapatite Nanoparticles in Dentinal Tubule Occlusion and Resistance to Erosive Wear (Scanning Electron Microscopic Study)

“…Unfortunately, no hypersensitivity therapy has been demonstrated to be entirely successful for all patients. (14) Consequently, it is mandatory to develop an occlusion agent that promotes the development of a deep and compact mineralization occlusion layer. (4) The optimal desensitizing agent should not irritate or jeopardize the dental pulp's structural integrity, be painless, both during and after application, be simple to use, show quick results, be long-lasting or permanent, and not harm the teeth or gingiva.…”

Effectiveness of Three Agents in Pulpotomy Treatment of Permanent Molars with Incomplete Root Development: A Randomized Controlled Trial

“…Nano-hydroxyapatite (NHA), another biocompatible and bioactive biomaterial, is an attractive inorganic material for endodontic application thanks to its chemical composition, biomimetic and nanoscale topography and its positive proliferative action on several mesenchymal cell types [ 28 , 29 , 30 ]. This biomaterial was used in humans as a pulp capping agent [ 31 , 32 ] and in pigs as pulp capping and pulpotomy material [ 33 ].…”