Effect of chemical enhancers and iontophoresis on thiocolchicoside permeation across rabbit and human skin in vitro

Artusi, Mariella; Nicoli, Sara; Colombo, Paolo; Bettini, Ruggero; Sacchi, Antonia; Santi, Patrizia

doi:10.1002/jps.20152

Cited by 49 publications

(25 citation statements)

References 29 publications

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“…Also rabbit back skin has been tested in vitro for passive permeability: some authors found rabbit skin more permeable [6,12] , while others found a passive permeability comparable to human skin [13,14] . Starting from the experience of other groups [12] on rabbit pinna skin, we have extensively used the skin coming from the inner part of the rabbit ear in in vitro permeation experiments [15][16][17][18][19][20] . This skin model is characterized by a density of hair follicles (80/cm 2 [16] ) much lower than that of the skin of the rabbit back and of other rodents (rat 8,000/cm 2 [21] ).…”

Section: Introductionmentioning

confidence: 99%

“…Rabbit ear skin proved to be a reasonable model for human skin, due to the comparable permeability shown by different molecules (lidocaine [16] , triptorelin [18] , thiocolchicoside [15] ). One of these works [16] has demonstrated also that rabbit ear skin is a reasonable model for studying the iontophoretic transport of drugs in vitro, since the relative electro-osmotic and electrorepulsive contributions were almost the same for human and rabbit skin.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Rabbit Ear Skin as a Skin Model for in vitro Transdermal Permeation Experiments: Histology, Lipid Composition and Permeability

Nicoli

Padula

Aversa

et al. 2008

Skin Pharmacol Physiol

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Aim: The aim of this work was to characterize rabbit ear skin in view of its use in transdermal permeation experiments. Method: The characterization included histological analysis of the tissue, qualitative and quantitative analysis of stratum corneum (SC) lipids, differential scanning calorimetry and permeation experiments (caffeine, nicotinamide, progesterone). As a reference, pig ear skin was used. Results: The results obtained show that rabbit ear skin has a similar SC thickness compared to pig skin although the viable epidermis has a different structure. The lipid composition of rabbit SC was similar to pig SC but was characterized by a lower content of ceramides and a higher content of cholesterol esters and triglycerides. In terms of permeability, rabbit ear skin was 4–7 times less permeable to hydrophilic compounds, probably because of the higher lipophilicity of its SC. The permeability to progesterone was comparable between isolated pig epidermis and rabbit ear skin. Conclusion: Overall, the results obtained in this work support the usefulness of rabbit ear skin as barrier for skin penetration studies, for both lipophilic and hydrophilic permeants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Rabbit Ear Skin as a Skin Model for in vitro Transdermal Permeation Experiments: Histology, Lipid Composition and Permeability

Nicoli

Padula

Aversa

et al. 2008

Skin Pharmacol Physiol

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“…Also, Transcutol P allows greater solubilization in the aqueous phase of the skin tissues. [50,51] Anti-inflammatory study Carrageenan-induced hind paw edema is the standard experimental model of acute inflammation. Carrageenan is the phlogistic agent of choice for testing inflammatory drugs as it is not known to be antigenic and is devoid of apparent systemic effects.…”

Section: Construction Of Pseudo-ternary Diagramsmentioning

confidence: 99%

Enhancement of skin permeation and anti-inflammatory effect of indomethacin using microemulsion

Barakat¹,

El-Medany²,

Fouad³

2011

Asian J Pharm

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T he objective of the present study was to investigate the potential of microemulsion formulations for transdermal delivery of indomethacin (IND). Microemulsion formulations with different surfactant:cosurfactant ratios (S mix ); F1-F6 (1:1, 2:2, 3:1, 4:1, 1:2, and 3:2) were prepared by the spontaneous emulsification method, and characterized for morphology, droplet size, and rheological characteristics. The ex vivo skin permeation studies were performed using Franz diffusion cell with rabbit skin as a permeation membrane. A significant increase in the steady-state flux (J ss ), permeability coefficient (K p ), and enhancement ratio (E r ) was observed in microemulsion formulations compared with the conventional IND gel. The antiinflammatory effects of microemulsion formulations showed a significant increase in percent edema inhibition value after 4 hours. The optimized formulation showed a significant increase in the steady-state flux (J ss ) and permeability coefficient (K p ). The enhancement ratio (E r ) was found to be 8.939 in optimized formulation F1 compared with IND gel.

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“…Accordingly, chemical (transcutol and lauric acid) or physical (iontophoresis) penetration enhancers have been considered to promote permeation of TCC across full-thickness human skin and epidermis (3). Miotens ® foam contains propylene glycol and propylene glycol diperlargonate as skin penetration enhancers.…”

Section: Introductionmentioning

confidence: 99%

Penetration and Distribution of Thiocolchicoside through Human Skin: Comparison Between a Commercial Foam (Miotens® ) and a Drug Solution

et al. 2008

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Abstract. Penetration and distribution of thiocolchicoside from a commercially available foam (Miotens ® 0.25%, w/v) through human excised full-thickness skin were evaluated using two different in vitro apparatus: a Franz diffusion cell and a Saarbruecken penetration model-based cell. In order to evaluate the intrinsic capability of the drug to penetrate into the skin, a simple drug aqueous solution prepared at the same drug concentration as Miotens ® was also tested. Results showed that both apparatus were suitable to study thiocolchicoside penetration into human skin. Penetrated drug amounts were comparable using the two apparatus, probably because skin acts as "sink" for the drug. Miotens ® was found to significantly promote thiocolchicoside accumulation into full human skin thickness in comparison with the simple drug solution. The mixture of propylene glycol and propylene glycol diperlargonate contained into Miotens ® foam has been proven to be effective to promote penetration of thiocolchicoside into human skin.

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Effect of chemical enhancers and iontophoresis on thiocolchicoside permeation across rabbit and human skin in vitro

Cited by 49 publications

References 29 publications

Characterization of Rabbit Ear Skin as a Skin Model for in vitro Transdermal Permeation Experiments: Histology, Lipid Composition and Permeability

Characterization of Rabbit Ear Skin as a Skin Model for in vitro Transdermal Permeation Experiments: Histology, Lipid Composition and Permeability

Enhancement of skin permeation and anti-inflammatory effect of indomethacin using microemulsion

Penetration and Distribution of Thiocolchicoside through Human Skin: Comparison Between a Commercial Foam (Miotens® ) and a Drug Solution

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