Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue

Ortiz, Adriana Cassia; Assis, Amanda Freire; Duarte, Max Jordan; Oliveira, Fabíola Singaretti; Passos, Geraldo A. S.; Beloti, Márcio Mateus

doi:10.1002/jcb.28463

Cited by 21 publications

(17 citation statements)

References 72 publications

(129 reference statements)

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“…For example, canine ADMSCs have higher differentiation potential than MSCs from bone marrow, umbilical cord, amniotic membrane, or placenta ( 21 ). In contrast, in horses, chondrogenic ( 69 ) and osteogenic potentials ( 70 ) seem higher in bone marrow MSCs than in ADMSCs. These results indicate the possibility that MSCs from different animals have different properties indeed.…”

Section: Discussionmentioning

confidence: 83%

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

2021

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Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs.

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Section: Discussionmentioning

confidence: 83%

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

2021

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“…Moreover, it has been found to play a role in the regulation of stem cell density and neural differentiation during adult neurogenesis [35]. The next gene, IGSF10, has been previously detected to be upregulated during osteoblast differentiation, with an implied role in the ossification process [36]. Finally, COL6A6 has been implicated in the process of vascular smooth muscle cell differentiation from pluripotent progenitors [37].…”

Section: Discussionmentioning

confidence: 99%

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Jankowski

Kaczmarek

Wąsiatycz

et al. 2021

IJMS

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Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine.

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“…The selection of seeding cells is one of three critical elements of tissue engineering. Previous investigations have demonstrated that mesenchymal stem cells (MSCs) derived from bone marrow, fat tissues, umbilical cord, or peripheral blood can serve as potential seeding cell sources for bone tissue engineering 17‐20 . However, sources of these stem cells are limited and the invasive isolation procedure is associated with a risk of complications.…”

Section: Introductionmentioning

confidence: 99%

Urine‐derived stem cells loaded onto a chitosan‐optimized biphasic calcium‐phosphate scaffold for repairing large segmental bone defects in rabbits

et al. 2021

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The treatment of large segmental bone defects can be challenging for orthopedic surgeons. The development of bone tissue engineering technology, including the selection of seeding cells and the construction of scaffolds, provides a promising solution. In this study, we investigated osteogenic differentiation of human urine‐derived stem cells (hUSCs, a newly identified class of stem cells), and developed a novel porous hybrid scaffold using biphasic calcium phosphate (BCP) bioceramic ornamented with chitosan sponges (CS). We combined hUSCs with a CS/BCP hybrid scaffold to construct tissue‐engineered bone and evaluated whether the combination promotes bone regeneration in large segmental bone defects in rabbits. The study showed that hUSCs can differentiate into osteoblasts, and the hUSCs adhered, proliferated, and differentiated on CS/BCP hybrid scaffolds. Micro–computed tomography measurements, biomechanical detection, and histological analyses revealed that the combination of hUSCs and the CS/BCP hybrid scaffold enhanced bone regeneration more effectively compared with conventional pure BCP scaffolds, indicating that hUSCs can be used as a cell source for bone tissue engineering and that cell‐scaffold–based biomimetic bone may be a promising approach to the repair of bone defects.

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Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue

Cited by 21 publications

References 72 publications

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Urine‐derived stem cells loaded onto a chitosan‐optimized biphasic calcium‐phosphate scaffold for repairing large segmental bone defects in rabbits

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