Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures

Steiner, Neusa; Vieira, Felipe do Nascimento; Maldonado, Sara; Guerra, Miguel Pedro

doi:10.1590/s1516-89132005000800005

Cited by 64 publications

(54 citation statements)

References 24 publications

(21 reference statements)

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“…Stein et al (2010) also observed in non-embryogenic callus of Inga vera (guaba) non-viable cells that had the same morphology as that of Type 1 callus of Musa sp.…”

Section: Resultsmentioning

confidence: 71%

“…The yellow callus showing small clusters ( Figure 2B) had isodiametric cells, and those having large yellow clusters were isodiametric in shape ( Figure 2C). Strosse et al (2003) According to Strosse et al (2003), the formation of callus with yellow heterogeneous glomeruli in Musa sp. acquire two patterns, friable compact callus or embryogenic callus with individual embryos (few embryos).…”

Section: Resultsmentioning

confidence: 99%

“…According Canhoto et al (1996), the cell pro-embryos may be covered by vacuolated cytoplasm that can exist only in a small space between the plasma membrane and the tonoplast. Stein et al (2010) observed that in embryogenic callus, organelles such as mitochondria and rough endoplasmic reticulum occupied a peripheral position because they were affected by large vacuoles. No starch grains were observed in the cells of callus Types 1, 2 and 3.…”

Section: Resultsmentioning

confidence: 99%

“…To induce callus formation, the "scalps" having grown approximately 3 mm x 3 mm x 3 mm in size were transferred to medium ZZss ( Table 1). The growing conditions consisted of a 16 hour photoperiod at a temperature of 25 ± 1°C, as proposed by Strosse et al (2003). The callus were further morphologically characterised into three types (Type 1 -transparent watery callus, Type 2 -yellow callus with small clusters, Type 3 -yellow callus with large clusters) by five reviewers using a colour and texture questionnaire.…”

Section: Methodsmentioning

confidence: 99%

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Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã

et al. 2012

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ABSTRACT. This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 -transparent watery callus, Type 2 -yellow callus with small clusters, Type 3 -yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were isodiametric, which is a characteristic of embryogenic cells. The TEM analysis showed that Type 1 callus cells had thin walls, a large number of small vacuoles and dispersed cytoplasm. The Type 2 callus cells showed dense cytoplasm, large vacuoles and a large amount of mitochondria. The Type 3 callus cells had thick and intercellular spaces. Thus, the Type 2 callus cells had characteristics consistent with embryogenic callus cells.Keywords: scanning electron microscopy, transmission electron microscopy, Musa sp., cytology, embryogenesis.Análise morfológica e ultra-estrutural de diferentes calos de bananeira, cv. Prata anã RESUMO. Este trabalho foi realizado com o objetivo de caracterizar morfologicamente e ultraestuturalmente calos (scalp method) de bananeira. Genótipos de bananeira, cv. Prata anã, cultivadas in vitro, foram utilizados para indução de meristemas na base das folhas e formação de estruturas conhecidas como scalp, que foram transferidas para meio ZZs. Os calos obtidos foram caracterizados morfológica e ultraestruturalmente. Para as análises ultra-estruturais, foram coletadas, fixadas em Karnovsky e preparadas cinco amostras, que foram analisadas em microscópio eletrônico de varredura e de transmissão. Foi verificado formação de 3 tipos de calos (Tipo 1-calo transparente aquoso; Tipo 2-calo com glomérulos amarelos menores; Tipo 3-calo com glomérulos amarelos maiores). A análise por MEV mostrou que os calos Tipo1 apresentaram células alongadas; os calos Tipo 2 e Tipo 3 apresentaram células com formato isodiamétrico que condizem com características de calos embriogênicos. A análise por MET mostrou que os calos Tipo1 apresentaram parede delgada, grande quantidade de pequenos vacúolos e citoplasma disperso. Os calos Tipo 2 apresentaram citoplasma denso, vacúolos grandes e presença de mitocôndrias. Os calos Tipo 3 apresentaram parede espessa e espaços intercelulares. Assim, os calos Tipo 2 evidenciam características de calos embriogênicos.Palavras-chave: microscopia eletrônica de varredura, microscopia eletrônica de transmissão, Musa sp., citologia, embriogênese.

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“…Stein et al (2010) also observed in non-embryogenic callus of Inga vera (guaba) non-viable cells that had the same morphology as that of Type 1 callus of Musa sp.…”

Section: Resultsmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã

et al. 2012

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show abstract

“…(2006) reported that the methods used to evaluate the presence of viable and non-viable cells can be classifi ed in two groups: those that stain only the dead cells, and those in which only the living cells are coloured, because the colour is a product of cell metabolic activity. Acetocarmine enables inferring chromosomal integrity and characterization of viable cells (STEINER et al, 2005;MUNHOZ et al, 2008), while Evans Blue enters damaged cell walls in apoptosis process, allowing for identifi cation of nonviable cells (BHARGAVA et al, 2007). Viability can also be quantifi ed with activity assays, using for example the 2, 3, 5-triphenyltetrazolium chloride (TTC).…”

Section: Introductionmentioning

confidence: 99%