Morphological characteristics and cell viability of coffee plants calli

Pádua, Marlúcia Souza; Paiva, Luciano Vilela; Silva, Luciano Coutinho da; Livramento, Kalynka Gabriella do; Alves, Eduardo; Castro, Ana Hortência Fônseca

doi:10.1590/s0103-84782014000400014

Cited by 17 publications

(14 citation statements)

References 15 publications

(12 reference statements)

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“…Embryogenic calli can be distinguished from non-embryogenic calli based on their morphological characteristics ( Yang and Zhang, 2010 ; Padua et al, 2014 ; Silva et al, 2014 ). In general, embryogenic calli are yellow and friable and their cells are small, isodiametric, arranged in clusters, with a dense cytoplasm, a nucleus with salient nucleoli and rich in small amyloplasts.…”

Section: Introductionmentioning

confidence: 99%

“…In general, embryogenic calli are yellow and friable and their cells are small, isodiametric, arranged in clusters, with a dense cytoplasm, a nucleus with salient nucleoli and rich in small amyloplasts. Non-embryogenic calli are spongy and translucent showing cells that are more elongated, with the vacuole occupying a big volume of the cytoplasm, higher number of vesicles and absence of cytoplasmic organelles ( Figure 1 ) ( Gatica-Arias et al, 2008 ; Padua et al, 2014 ; Silva et al, 2014 ). Zygotic and somatic embryo development processes for all plant species are quite similar.…”

Section: Introductionmentioning

confidence: 99%

“…In the morphogenetic stage the structure of embryos are established and the second stage is marked by a pronounced biochemical activity. In dicots, the morphogenetic stage is divided in four phases; globular, heart, torpedo and cotyledonary stage ( Etienne, 2005 ; Yang and Zhang, 2010 ; Etienne et al, 2013 ; Padua et al, 2014 ). Despite that both zygotic and somatic embryogenesis are similar, there are some differences in morphological and histochemical aspects during embryo development.…”

Section: Introductionmentioning

confidence: 99%

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Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Campos

Panis²,

Carpentier

2017

Front. Plant Sci.

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One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied in order to provide practical results for coffee improvement. While genetic transformation got many attention in the past and is booming with the CRISPR technology, micropropagation and SE are still the major bottle neck and urgently need more attention. The methodologies to induce SE and the further development of the embryos are genotype-dependent, what leads to an almost empirical development of specific protocols for each cultivar or clone. This is a serious limitation and excludes a general comprehensive understanding of the process as a whole. The aim of this review is to provide an overview of which achievements and molecular insights have been gained in (coffee) somatic embryogenesis and encourage researchers to invest further in the in vitro technology and combine it with the latest omics techniques (genomics, transcriptomics, proteomics, metabolomics, and phenomics). We conclude that the evolution of biotechnology and the integration of omics technologies offer great opportunities to (i) optimize the production process of SE and the subsequent conversion into rooted plantlets and (ii) to screen for possible somaclonal variation. However, currently the usage of the latest biotechnology did not pass the stage beyond proof of potential and needs to further improve.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Campos

Panis²,

Carpentier

2017

Front. Plant Sci.

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“…Short, round and isodiametric cells coincide with those observed by several authors that define them as a common occurrence in viable cells of embryogenic calli. Padua et al (2014) also detected isodiametric cells with evident nucleus in friable Coffea arabica callus.…”

Section: Histologymentioning

confidence: 81%

Induction, Growth Kinetics and Morpho-histological Characterization of Neem Callus

Oliveira¹,

Oliveira²,

Machado³

et al. 2018

JAS

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Azadirachta indica A. Juss, popularly known as neem, is a species native to India, belonging to family Meliaceae, considered the most important plant species with insecticidal action. The aim of this study was to evaluate the influence of growth regulators on induction and growth of neem callus and to observe their viability for embryogenesis through morpho-histological characteristics. In vitro germinated plants were used for excision of nodal explants. These segments were inoculated in Murashige and Skoog culture medium containing 1.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic) combined with BAP (6-benzylaminopurine) at the following concentrations: 0.0, 0.5, 1.0 and 2.0 mg/l (T1, T2, T3 and T4 respectively), for callus induction. At 0 (mass of nodal segments without callus), 20, 40 and 60 days of culture, the percentage of callus formation was observed and the callus weight was measured for each treatment and at the end of the 60 days, consistency, color, and cell histology were evaluated. There was callus formation in all treatments tested. The highest induction of Azadirachta indica A. Juss callus is observed in the presence of 1.0 mg/l 2,4-D + 2.0 mg/l BAP, with callus showing light brown color, friable consistency and rounded cells with intense cell division, typical of cells with potential embryogenic capacity.

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“…Three months later, two different cell suspensions (Fig. ) considered as embryogenic based in morphological observations were used to extract the proteins. The embryo regeneration capacity was estimated based on two samples with the same morphological characteristics (Fig.…”

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confidence: 99%

The proteome profile of embryogenic cell suspensions of Coffea arabica L.

Campos

Paiva

Panis³

et al. 2016

Proteomics

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Somatic embryogenesis, is a process by which new viable embryos are produced from somatic tissues. Somatic embryogenesis is not only a useful biotechnological tool for the massive clonal propagation and genetic engineering but it also allows to obtain fundamental knowledge about the molecular changes that take place during embryogenesis. We present the proteome profile of two embryogenic cell suspensions. We identified 1052 non-redundant proteins. We present their known GO annotations and show two protein networks sharing the GO annotations related to stress and embryogenic capacity via the free program Cytoscape. To our knowledge these results give the first high-throughput proteome description of embryogenic cell suspensions and provide new information about somatic embryos for the whole plant community. The published proteome is a first step toward understanding somatic embryogenesis in coffee and toward a better annotation of proteins in an important non-model crop. All data are available via ProteomeXchange with identifier PXD002963.

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Morphological characteristics and cell viability of coffee plants calli

Abstract: The a im of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá

Cited by 17 publications

References 15 publications

Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Induction, Growth Kinetics and Morpho-histological Characterization of Neem Callus

The proteome profile of embryogenic cell suspensions of Coffea arabica L.

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