Effect of Carbon Source in Alginate Bead on Synthetic Seed Germination in Eggplant (Solanum melongena L.)

., A.K.M.N. Huda; ., M.H. Rahman; Bari, M. A.

doi:10.3923/jps.2007.538.544

Cited by 11 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rao and Singh [81] encapsulated somatic embryos in various concentrations (2-6 percent) of sodium alginate and complexed with calcium chloride (25-100 mM), and discovered that 3 percent sodium alginate and 75 mM calcium chloride were best for encapsulation. Huda et al, [82] produced synthetic seeds using nodal segments and somatic embryos in two cultivars of eggplant utilizing varying quantities of sucrose, mannitol, and sorbitol in an alginate seed bead to optimize synthetic seed germination on MS media. Huda and Bari [83] used somatic embryos from the same two varieties to standardize the synthetic artificial endosperm media composition.…”

Section: Cucumber (Cucumis Sativus L)mentioning

confidence: 99%

Production of Synthetic Seeds in Vegetable Crops: A Review

Abbas

Mahood

Alhasan

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Over the last few decades, biotechnology research has advanced to the point where it is now possible to improve essential agricultural crops. Synthetic seed manufacturing has opened up new possibilities of in vitro plant propagation technique since it has various practical benefits on a commercial scale for the cultivation of a wide range of agricultural crops. It is one of the most important tools for plant tissue culture breeders and scientists, since it provides significant benefits for massive production of distinct (elite) plant species. Synthetic seeds offer a promising strategy for mass plant production in a way of encapsulation of non-embryonic or embryonic (somatic embryos) tissues in a gel-like matrix, such as shoot buds, shoot tips, microshoots, protocoms and nodal segments. When the strategies minimize the cost of the production of elite plant genotypes, this strategy can be adapted for commercial production. The technology of the synthetic seed necessitates the use of in vitro growth systems in order to produce viable materials that can be converted into plants on a wide scale. The discovery of an artificial seed technology opens up new possibilities of a promising strategy for improving a wide range of commercially significant plant species, including cereals, fruit crops, medicinal plants, and vegetable crops, which we focus on in this review article.

show abstract

Section: Cucumber (Cucumis Sativus L)mentioning

confidence: 99%

Production of Synthetic Seeds in Vegetable Crops: A Review

Abbas

Mahood

Alhasan

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Different kinds of encapsulated propagules might respond differently to short-term cold storage. For example, the encapsulated somatic embryos of eggplant and Catharanthus roseus could be stored for 45, and 75 days, respectively [8,25], the encapsulated protocorms of Cymbidium aloifolium and the nodal buds of Aquilaria malaccensis could be stored for 90 days and 60 days, respectively [24,26]. The encapsulated microshoots of rice could be stored at 4 • C for 30 days [15], while those of Begonia and Nerium oleander could be stored at 4 • C for 56 days [16,27].…”

Section: Germination and Viability Assessmentmentioning

confidence: 99%

“…If required, the encapsulated propagules or commonly called "synthetic seeds," could possibly be used to facilitate short-term storage required for germplasm exchange or delayed sowing [5,6]. In most studies on synthetic seeds, a plant propagule is often enclosed within a single alginate coating [7,8], while variations of this approach, including double coating layers, have also been investigated [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Phanomchai

Bodhipadma

Noichinda

et al. 2022

Plants

View full text Add to dashboard Cite

Microshoots have been widely used for micropropagation. It may be necessary to store microshoots for a short period of time, for example in germplasm exchange needing transport to other research groups. Here, we investigated the short-term storability of alginate-encapsulated Persian violet (Exacum affine Balf. f. ex Regel) microshoots at 4 °C and 25 °C. After storage, the encapsulated microshoots were sown on basal Murashige and Skoog medium for germination and viability determination using tetrazolium chloride staining. The results showed that one or five microshoots encapsulated with a single alginate layer could be stored at 4 °C for up to 30 days, while the percentages of germination and viability of the microshoots encapsulated with two layers of alginate were greatly reduced upon storage. This is the first report on the storability of alginate-encapsulated multiple microshoots, which could be a more efficient way to encapsulate microshoots used for short-term cold storage.

show abstract