Effect of Carbohydrate Source on Growth and Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets

Sumaryono, Sumaryono; Muslihatin, Wirdhatul; Ratnadewi, Diah

doi:10.4308/hjb.19.2.88

Cited by 30 publications

(19 citation statements)

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“…However, sucrose at high concentrations can be toxic and inhibit the growth and development of plants. This phenomenon was observed, for example in Metroxylon sagu plants grown in vitro (Sumaryono, Muslihatin & Ratnadewi, 2012).…”

Section: Discussionmentioning

confidence: 76%

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

Ptak

Morańska

Skrzypek

et al. 2020

PeerJ

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Background Leucojum aestivum L. is an important medicinal plant which produces Amaryllidaceae alkaloids, especially galanthamine and lycorine. Research is currently exploring the possibility of producing these alkaloids using biotechnological methods, including in vitro cultures. The biosynthesis of alkaloids may be affected by the types and concentrations of carbohydrate sources used in the medium. In the present investigation we performed such studies on in vitro cultures of L. aestivum with a view to obtaining plant material of good quality, characterized, in particular, by a high content of valuable Amaryllidaceae alkaloids. Methods We examined the effects of various types of carbohydrate sources—sucrose, glucose, fructose and maltose—at different concentrations (30, 60 and 90 g/L)—on the quality of L. aestivum plants grown in the RITA® bioreactor. The plants’ quality was assessed by their biomass increments, as well by as analysing photosynthetic pigments, endogenous sugar, phenolics and Amaryllidaceae alkaloid content. We also investigated the effect of sugars on the activity of the antioxidant enzymes catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Results The highest biomass increments were observed in plants cultivated in the medium containing 90 g/L sucrose. The highest CAT activity was noted in cultures growing in the medium supplemented with 90 g/L maltose, while the highest POD activity was observed in the presence of 90 g/L fructose and 60 g/L maltose. No differences in SOD activity were observed. Moreover, the sugars did not affect the contents of chlorophyll a and carotenoids, whereas the highest amount of chlorophyll b was recorded in plants growing in the medium with 60 g/L maltose. No statistically significant differences were observed in the contents of endogenous sugars and phenolics in any in vitro conditions. However, the addition of sugar had a decisive effect on the biosynthesis of the Amaryllidaceae alkaloids. The highest distribution of alkaloids occurred in plants cultured in the medium containing 60 g/L sucrose. Six Amaryllidaceae alkaloids were detected in the plant tissue. The addition of 30 g/L fructose in the medium resulted in the accumulation of five alkaloids, including ismine, which was not identified in other analysed tissues. The highest concentration of galanthamine was observed in plants cultured in the presence of 30 g/L fructose and 60 g/L sucrose (39.2 and 37.5 µg/g of dry weight (DW), respectively). The plants grown in the medium containing 60 g/L sucrose exhibited the highest lycorine content (1048 µg/g of DW). Conclusions The type and concentration of sugar used in the medium have an essential influence on the biosynthesis of Amaryllidaceae alkaloids in L. aestivum plants cultured in a RITA® bioreactor. The results point to an interesting approach for commercial production of galanthamine and lycorine.

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Section: Discussionmentioning

confidence: 76%

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

Ptak

Morańska

Skrzypek

et al. 2020

PeerJ

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“…They suggested that in vitro culture of sago palm is more favorable in an environment with high solute concentration or low osmotic potential. However, results reported by Sumaryono et al (2012) revealed that increasing the concentration of sucrose or other carbohydrates did not favor the growth and vigor of sago plantlets. Rooting of the plantlets was conducted in liquid medium as commonly performed in oil palm plantlets (Konan et al 2007).…”

mentioning

confidence: 83%

EFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLI

Riyadi¹,

Sumaryono²

2017

Indones. J. Agric. Sci.

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<p>The application of gamma irradiation on plant materials may increase the genetic variation of the offspring with useful traits. The experiment was conducted to determine the effect of irradiation dosage of gamma ray on growth and development of sago palm (Metroxylon sagu) calli. Friable calli of sago palm derived from suspension culture were used as a material source. The primary calli were initiated from apical meristematic tissues of sago palm suckers of Alitir variety from Merauke, Papua. The treatments used were dosage of gamma ray irradiation at 0, 5, 10, 15, 20 and 25 Gy. The treated calli were then subcultured on modified Murashige and Skoog (MMS) solid medium containing 3% sucrose and 0.1% activated charcoal and added with 1 mg l-1 2,4-D and 0.1 mg l-1 kinetin. The results showed that at all irradiation dosages, calli biomass increased significantly. The highest proliferation of calli biomass of 5.33 folds from the initial culture after 4 weeks was achieved at gamma irradiation of 25 Gy, whereas the lowest proliferation of calli biomass of 3.4 folds was achieved at control. The best development of embryogenic calli was obtained at 10 Gy that produced 100% somatic embryos, whereas the lowest somatic embryo formation at 0% was obtained at 0 and 25 Gy after one subculture. High response of somatic embryo induction to gamma irradiation at 10 Gy may increase production of somatic embryos. These results can be used in in vitro breeding of sago palm via mutagenesis to create new elite varieties.</p>

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“…Carbohydrate content as photosynthate is low in long photoperiods but the effects is varied according to cultivar [14]. Photosynthate accumulation in the trunk can increase the height, diameter at the same plant fresh weight [15] [16].…”

Section: Resultsmentioning

confidence: 99%

Effects of Photoperiodism to The Growth Rate of Hibiscus sabdariffa L

Muslihatin

Daesusi

2014

JTS

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AbstractRed roselle (Hibiscus saddariffa L.) is a short day plant, this plant is only cultivated in a certain time period. There is a need to manipulate the environment where this plant can grow. The objective of this research was to study the influence of day length tothe relative growth rate (RGR) of H. sabdariffa L. Day length treatment was carried out by covering plants by black cover. Day length treatments were given in 8, 9,10, 11 and 12 hours of light exposure. Parameters measured were RGR of height plant, diameter of stem, number, and color of leaves. Harvesting was carried out when the flower crown fell or petals furl (the age of plants reached 3-4 months). The result of this research showed that highest plant was obtained at 10 hours day length (128.20 cm), the largest stem diameter growth rate was obtained at 8 hours (1.79 cm), number of leaves at 10 hours (183.40),and green color of leaves on 12 hours (scale 4). The highest RGR was obtained at 8 hours for all parameters except the height of plant and the color of leaves. Plant height, diameter and number of leaves at 8,9,10, and 11 hours of day length did not show significance difference. This showed that roselle is a short day length plant that grow optimal under 12 hours of day length. Kata kunciHibiscus sabdariffa L., panjang hari penyinaran, LPR

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Effect of Carbohydrate Source on Growth and Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets

Cited by 30 publications

References 9 publications

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

EFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLI

Effects of Photoperiodism to The Growth Rate of Hibiscus sabdariffa L

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Effect of Carbohydrate Source on Growth and Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets

Cited by 30 publications

References 9 publications

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA®bioreactor

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA®bioreactor

EFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLI

Effects of Photoperiodism to The Growth Rate of Hibiscus sabdariffa L

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Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor