Effect of calcium ion on fatty acid-induced generation of superoxide in guinea pig neutrophils.

Morimoto, Yosuke; Sato, Eisuke F.; Nobori, Koichi; Takahashi, Ryutaro; Utsumi, Kozo

doi:10.1247/csf.11.143

Cited by 13 publications

(4 citation statements)

References 41 publications

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“…In accordance with other investigators [1,[44][45][46][47][48][49], we found clear differences between various structurally different FA and their ability to induce ROS generation in unstimulated neutrophils, suggesting that FA distinctively influence neutrophilfunction depending on the number of double bonds. N-6 fatty acids with known proinflammatory characteristics showed a clear potency to generate the production of ROS, whereas this effect was much lower with OA.…”

Section: Discussionsupporting

confidence: 92%

“…In addition, intracellular calcium elevation was found to be important but not essential for the AA-induced ROS generation which supports the hypothesis of previous reports showing that Ca 2+ mobilization induced by C20:4 (n-6) may be partly but not totally involved in ROS production [37]. It should be mentioned here that other groups have found a poor correlation between the degree of Ca 2+ mobilization and the amount of superoxide produced [43] or even an enhanced Ca 2+ rise in the absence of extracellular calcium [46] making it difficult to conclude the role that Ca 2+ plays in regulating superoxide production in response to n-6 FA.…”

Section: Discussionsupporting

confidence: 89%

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Activation of Human Neutrophils by Oleic Acid Involves the Production of Reactive Oxygen Species and a Rise in Cytosolic Calcium Concentration: a Comparison with N-6 Polyunsaturated Fatty Acids

Carrillo

Cavia

Roelofs

et al. 2011

Cell Physiol Biochem

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Background: There is a growing body of evidence showing that dietary constituents and lipids in particular, influence the function of the human immune system. However, although the beneficial effects of oleic acid (OA) are clear, its mechanism of action at the molecular level is poorly understood. Aims: To evaluate neutrophil activation under the influence of OA and compare this with several n-6 PUFAs. Methods: Two key aspects of neutrophil activation were investigated: oxygen radical (ROS) production and intracellular Ca²⁺ signaling. Results: OA and the n-6 PUFA arachidonic acid (AA) both induced ROS production in a dose-dependent manner, although AA was the more potent stimulus. When looking for the mechanisms behind these effects, we found that both FA induce increases in cytosolic calcium concentration [Ca²⁺]_i), but whereas OA-induced ROS production is totally mediated through Ca2+ signaling, this is not the case for AA since ROS generation by AA is only partly inhibited in BAPTA-treated cells. We also found evidence for the involvement of protein kinase C (PKC) in the OA-induced ROS generation; by contrast, other enzymes apart from PKC seem to be implicated in n-6 PUFA-induced ROS production. In addition, our results argue against the involvement of a pertussis toxin-sensitive receptor activated by OA. Conclusions: OA differs from the n-6 PUFA AA in the activation of human neutrophils and these differences may be related to their distinct inmunomodulatory properties.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Activation of Human Neutrophils by Oleic Acid Involves the Production of Reactive Oxygen Species and a Rise in Cytosolic Calcium Concentration: a Comparison with N-6 Polyunsaturated Fatty Acids

Carrillo

Cavia

Roelofs

et al. 2011

Cell Physiol Biochem

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“…OPMN and RPMN were suspended in Krebs-Ringer phosphate buffer (KRP) (1 ϫ 10 6 cells/ml) and kept on ice until used for the experiments. Rat peritoneal neutrophils (RPMN) were obtained 16 h after intraperitoneal injection of 2% casein as described previously (17).…”

Section: Methodsmentioning

confidence: 99%

Analysis of Reactive Oxygen Species Generated by Neutrophils Using a Chemiluminescence Probe L-012

Imada

Sato

Miyamoto

et al. 1999

Analytical Biochemistry

131

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“…Several studies have reported that unsaturated fatty acid effects the ROS production in neutrophils (24,34,(37)(38)(39)(40)(41)(42)(43)(44)(45)(46). Nevertheless, fatty acids can develop or inhibit the development of neutrophil activation depending on the experimental conditions.…”

Section: Production Of Reactive Oxygen Species (Ros)mentioning

confidence: 99%

Effects of oleic acid

Karacor

Cam

2015

Med Sci and Discov

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Content of fatty diet plays a significant role in the development and progression of chronic diseases. Generally, it is accepted that unsaturated fatty acids are beneficial. According to literature review, oleic acid (C18:1n-9) has positive impacts on various tissues in general and has negative impacts rarely. Olive oil composition includes high amount oleic acid. Considering of the effects of oleic acid on the cardiovascular system, it was determined that decreasing of the myocardial infarction rate, platelet aggregation and secretion of TXA2, plus reduce of the systolic blood pressure. LDL cholesterol was decreased. In terms of effects on the liver, while some studies suggest that oleic acid has beneficial effects, unlike other studies proved that exposed to oleic acid of hepatocytes induce ER (endoplasmic reticulum) stress in long-term period. The several studies which were investigating the efficacy of the oleic acid on the tumor tissue proved that OA increased to hepato-tumorigenesis in vivo as a potential. On te other hand, another research showed that oleic acid blocked the action of HER-2 / neu oncogene that led to breast cancer. Although some researchers reported that OA develop neutrophil phagocytic capacity and candidacidal activity, other researchers point out that these fatty acids didn't cause any changes on bactericidal activity and fatty acids caused moderate decreases on phagocytosis and chemotaxis only in extremely high concentrations, and they suppressed to T lymphocytes. Although, most of studies have indicated to beneficial effects of oleic acids, also the adverse effects of oleic acid have been reported in a few studies. This situation requires further researches for detail information about oleic acid

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Effect of calcium ion on fatty acid-induced generation of superoxide in guinea pig neutrophils.

Cited by 13 publications

References 41 publications

Activation of Human Neutrophils by Oleic Acid Involves the Production of Reactive Oxygen Species and a Rise in Cytosolic Calcium Concentration: a Comparison with N-6 Polyunsaturated Fatty Acids

Activation of Human Neutrophils by Oleic Acid Involves the Production of Reactive Oxygen Species and a Rise in Cytosolic Calcium Concentration: a Comparison with N-6 Polyunsaturated Fatty Acids

Analysis of Reactive Oxygen Species Generated by Neutrophils Using a Chemiluminescence Probe L-012

Effects of oleic acid

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