Effect of bonding application time on the microleakage of Class V sandwich restorations

Abstract

Background: The aim of this study was to assess the effect of bonding application time on the microleakage of Class V sandwich restorations. Methods: Eighty non-carious third molars were randomly divided into 16 groups. Two Class V cavities were prepared on the buccal and lingual surfaces of teeth. Three groups were restored with Fuji II GIC and treated with a total-etch bonding system (Stea ⁄ SDI) immediately after insertion, at 7 minutes and 15 minutes after mixing the glass ionomer cements (GICs). Another three groups were restored with Riva Self Cure GIC and treated with the total-etch system identically. The other six groups were subjected to self-etching bonding (Frog ⁄ SDI) after GIC placement in an identical procedure. The remaining groups were made using light cure GICs (Fuji II or Riva Light Cure) in conjunction with the total-etch or self-etching systems. Cavities were then restored with composite (Valux plus, 3M ⁄ ESPE). Samples were subsequently immersed in 2% methylene blue solution for 48 hours and observed under a stereomicroscope after sectioning. Four-scale grading was used to assess microleakage in occlusal and gingival walls. Data were analysed using Kruskal-Wallis and Mann-Whitney tests. Results: The self-etching bonding system exhibited more microleakage in occlusal margins regardless of time. Over time, microleakage significantly decreased in gingival margins in all self-cure groups except for Riva Self Cure treated with the total-etch system (p < 0.05). Conclusions: Bonding application time had no effect on the microleakage of occlusal margins. However, maturation of GICs induced a decreased microleakage in gingival margins.

Effect of Pit and Fissure Sealants on the Prevention of Enamel Demineralization After Exposure to Streptococcus mutans Biofilm: An Vitro Study

“…[4,18,28,29] Retention in and penetration into pits and fissures, risk of isolated carious lesions, and the inherent properties of the materials are some of the factors that can affect the effectiveness of a sealant. [19,20,21] In the present study, fluoride-releasing materials interfered with the metabolic activity of the biofilm, confirming the antimicrobial activity of fluoride. [24] The model used in this study for prevention of demineralization might have contributed to the results found for the dental materials tested.…”

“…Several studies have used different methodologies to compare the application of ionomeric and resinous materials to pits and fissures in an attempt to prevent demineralization. [17,[20][21][22] Therefore, in this study, a cavity preparation was performed to simulate the conditions of pits and fissures and the Knoop microhardness test was carried out to assess demineralization around the preparation margins after 48-hour biofilm formation. Knoop microhardness has been widely used for this purpose [18,23,24] and the S. mutans biofilm metabolic activity test, which can simulate oral conditions, has been used to replace cyclic pH.…”

Microleakage at enamel and dentin margins with a bulk fills flowable resin

“…[17] Dye penetration is an established in vitro method for investigating marginal leakage along tooth-restoration interfaces and is generally assessed after cutting the teeth in the longitudinal direction. [181920] Various tracer dyes are available for microleakage studies and there appears to be no significant difference in tracer penetration among fuchsin, silver nitrate and methylene blue. [21] Methylene blue is one of the most common tracers and can be used at different concentrations.…”

Evaluation of Microleakage and Microtensile Bond Strength of Two Bulk Fill Composites in Primary Teeth after Caries Removal by Chemomechanical Technique

“…Microleakage can be assessed by scanning electron microscope of dye penetration. Penetration of dye is an in vitro method to investigate marginal leakage over tooth restoration interfaces (12)(13)(14) . Different tracer dyes are available for micro leakage researches, while there is no important difference in penetration of tracer between them (15) .…”