Effect of blood glucose level on acute stress response of grass carp Ctenopharyngodon idella

Abstract

Stress has a considerable impact on welfare and productivity of fish, and blood glucose level of fish may be a factor modulating stress response. This study evaluated the effect of blood glucose level and handling on acute stress response of grass carp Ctenopharyngodon idella. Fish were intraperitoneally injected with glucose at 0, 0.2, 0.5, and 1.0 mg g body mass (BM) and then were exposed to handling for 5 min. Glucose injection resulted in increase of plasma glucose level and liver glycogen content and decrease of plasma lactate level. Handling resulted in increase of plasma levels of cortisol, glucose, and lactate and plasma lactic dehydrogenase (LDH) activity and decrease of liver glycogen content. At 1 h post-stress, the plasma cortisol level was lower in the stressed fish injected with glucose at 0.5 mg g BM than the stressed fish injected with glucose at 0, 0.2, and 1.0 mg g BM. No significant differences were found in the activities of phosphoenolpyruvate carboxykinase (PEPCK) and pyruvate kinase (PK) in the liver between the stressed and unstressed fish, regardless of the dose of glucose injection. At 1 h post-stress, the liver glucose-6-phosphatase (G6Pase) activity was higher in the fish without glucose injection than in the fish injected with glucose. This study reveals that blood glucose level can affect stress response of grass carp by modulating cortisol release and glucose homeostasis through glycogen metabolism and gluconeogenesis in the liver.

The effects of differences in water exchange on the productivity of glass eel stage in Anguilla bicolor bicolor culture

“…Blood glucose plays an essential role as an indicator of fish physiology, especially the stress response on grass carp fish [17]. Similar research has also been reported that the glucose level in fish is related to the stress response caused by some factors such as high stocking density [18], environmental disturbance [19], salinity [20], and pollutant of heavy metal [20].…”

Impact of transient temperature disturbance on the oxidative stress indices and glucose levels of juvenile Koi carps (Cyprinus carpio var koi)

“…Glucose is majorly involved in necessity-based transformation of chemical to mechanical energy. Being a primary energy molecule, it is extremely sensitive to varying levels of stress and its regulation is done according to the feedback mechanism (Jiang, Wu, Huang, Ren, & Wang, 2017). Wells and Pankhurst (1999) have also reported a positive correlation of stress with glucose.…”

Ameliorative Effects of Spirulina platensis and Ulva sp. on Biochemical Aspects and Residues of Lambda-Cyhalothrin in the Water of Nile Tilapia (Oreochromis niloticus)

“…The blood glucose level of fish may be a factor modulating stress response and highly dependent on feeding status. Jiang et al, (2017) stated that the increased glucose concentrations may cause hyperglycemia due to the reaction of the hormone affected by stress. Such a raise may be due to the increased reaction of stressed fish to gluconeogenesis to meet their additional energy requirement.…”