Effect of Black Grape Seed Extract (Vitis vinifera) on Biofilm Formation of Methicillin-Resistant Staphylococcus aureus and Staphylococcus haemolyticus

Al–Mousawi, Alia Hussein; Al-kaabi, Siham Jasim; Albaghdadi, Ahmad J.H.; Almulla, Abbas F.; Raheem, A Abd El; Algon, Ali Abbas Abo

doi:10.1007/s00284-019-01827-0

Cited by 19 publications

(10 citation statements)

References 32 publications

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“…The results are agreed with Ranjitha et al [66], The GSE was more effective against S. aureus (18 mm) followed by K. pneumonia (13 mm) and E. coli (11 mm) i.e., susceptibility to GSE was high in case of Gram positive bacterium when compared to Gram negative bacteria. In the study by Silvaa et al [67], Grape seeds extract presented higher antimicrobial activity than peels due to their higher contents of catechin, epicatechin, and trans-resveratrol additionally, Grape seeds extracts has been shown to have antibacterial activities against both Gramnegative and Gram-positive bacteria, and it's have a high concentration of flavonoids, which may help to reduce biofilm formation [68]. These differences in the antibacterial activity in this study, as well as in the other studies in the literature depend on the tested microorganism and the composition in the phenolic compounds of the extracts and on the existence of a synergetic effect between the different polyphenolic compounds with an antiradical role and antimicrobial activity [65].…”

Section: Antibacterial Activitymentioning

confidence: 97%

Biological activities of Egyptian grape and mulberry by-products and their potential use as natural sources of food additives and nutraceuticals foods

Abdel-Khalek

Mattar

2022

Food Measure

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Interest in the biological role of bioactive compounds present in plant by-products has increased over the last few years. This study aimed to investigate the nutritive value and biological activities of Egyptian Grape leaves (GL), Grape seeds (GS) and Mulberry leaves (ML), as well as investigate the impact of γ-irradiation for improving the utilization of these plant byproducts. The dose level 5.0 kGy showed highest the content of crude protein (24.42, 19.41 and 13.50 mg/100 g), as well as crude fiber (34.26 and 21.18 mg/100 g) for ML, GL and GS, respectively. Mulberry leaves has a highest content of protein and fiber at dose 5.0 kGy compared with GL and GS. The highest total phenolic content was found in GS (9.75 mg/g DW), followed by GL (7.32 mg/g DW) and the lowest in ML (5.97 mg/g DW). While ML had a higher total flavonoids content (5.61 mg/g DW) than GS (4.88 mg/g DW) and GL (2.86 mg/g DW). Total phenolic and flavonoid contents were significantly increased at 5.0 kGy. The highest level (83.25% and 80.24%) of scavenging activity (DPPH %) and inhibition activity of HCT 116 cells was recorded at 5.0 kGy by GS. All extracts irradiated at 5.0 kGy exhibited varying degrees of antibacterial activity against (Gram+ve and Gram-ve), the GS followed by GL then ML showed strong antibacterial activity with a diameter of inhibition zone of 26.2, 24.5 and 19.7 mm, against L. monocytoganes, respectively and 24.4, 21.4 and 17.2 against S. typhimurium, respectively. This study suggests that γ-irradiation is an effective technique to enhance the recovery of phenolics and flavonoids from GL, GS and ML. Also in current study, antioxidant, antibacterial and anticancer activity has been suggested to appear a clear positive relationship with the total phenolic material. This study has proved that the Egyptian GL, GS and ML are rich sources of valuable phytochemicals and nutrients that can serve as a potential source of nutraceuticals and multifunctional food additives (antimicrobial, antioxidant, and anticancer). Phenolic compounds recovered from GL, GS and ML may have a potential role in fighting the COVID-19.

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Section: Antibacterial Activitymentioning

confidence: 97%

Biological activities of Egyptian grape and mulberry by-products and their potential use as natural sources of food additives and nutraceuticals foods

Abdel-Khalek

Mattar

2022

Food Measure

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“…Global grape production is estimated to be about 75 million metric tons per year (Rajabi Hamedani et al 2019). Considering the low cost to collect and process the grape seeds in addition to its reported therapeutic phyto-composition that aids cardio protective (Zhang et al 2016), anti-inflammatory (Lin et al 2017), anticancer (Kumar et al 2018;Di Meo et al 2019), anti-microbial (Fiallos et al 2020;Memar et al 2019;Al-Mousawi et al 2020), anti-oxidant (Kwatra 2020), antiviral (Kwatra 2020), neuroprotective (Zhao et al 2020), hepatoprotective (Nassiri-Asl and Hosseinzadeh, 2016), and wound-healing properties (Moalla Rekik et al 2016), it is currently gaining attention for therapeutic use in pharmaceutical industries (Rodríguez-Pérez et al 2019). Extracts obtained from grape seed contains large quantities of monomeric phenolic compounds such as (−)-epicatechin, (+)-catechins, and (−)-epicatechin-3-o-gallate and dimeric, trimeric, and tetrameric proanthocyanidins (condensed tannins) (Mohammed et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Incorporation of grape seed extract towards wound care product development

Ajit¹,

Vishnu²,

Varkey

2021

3 Biotech

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Naturally derived ingredients are becoming more prevalent in therapeutic drug formulations due to consumers' concerns about chemical side effects. In the context of wound care, despite the impressive progress in therapeutic product development, drugs dispensed to treat impaired healing challenged by biofilms; excessive inflammation and oxidation are not yet really effective. Thus, the hunts for improved drug formulations preferably using natural ingredients that are cost-effective in accelerating the wound-healing process are of constant demand. The grape seed extract is extensively studied and is reported to be rich in phenolic compounds, unsaturated fatty acids and vitamins which exhibit numerous therapeutic benefits owing to their anti-inflammatory, anti-microbial, and anti-oxidative properties that support its potential use in the development of wound-healing products. We conducted a literature study using Scopus, PubMed, and Google Scholar including the keywords "grape seed extract" and "wound healing". We also scanned all the references cited by the retrieved articles. Accordingly, this review is aimed to (i) explore the various phytochemical constituents found in grape seed extracts along with their mechanism of action that instigate wound healing, (ii) to highlight the latest pre-clinical and clinical assessments of grape seed extract in wound models, and (iii) to encourage innovation scientists in the field to address current limitations and to effectively develop grape seed extract-based wound care product formulations for commercialization.

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“…Along similar lines, it has been observed by the German Neonatal Network (GNN) that weekly screening for MDR colonizers has brought down the rate of LOS essentially due to the decrease in CoNS colonization [15]. It is also noteworthy to observe that with the rise in ß-lactam resistance and mecA gene carriage among clinical S. haemolyticus isolates, numerous combination therapies are increasingly being devised to treat LOS [16,17].…”

Section: Introductionmentioning

confidence: 76%

Influence of Sub-Inhibitory Dosage of Cefotaxime on Multidrug Resistant Staphylococcus haemolyticus Isolated from Sick Neonatal Care Unit

Chakraborty

Bardhan

Basu³

et al. 2022

Antibiotics

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Staphylococcus haemolyticus has emerged to be a frequently encountered late-onset sepsis pathogen among newborn infants. Critical care of neonates involves substantial usage of antibiotics and these pathogens are often exposed to sub-optimal doses of antibiotics which can augment maintenance of selection determinants and a range of physiological effects, prime among them being biofilm formation. Therefore, in this study, the outcome of a sub-inhibitory dosage of a commonly prescribed third-generation antibiotic, cefotaxime (CTX), on multidrug resistant (MDR) S. haemolyticus, was investigated. A total of 19 CTX-resistant, MDR and 5 CTX-susceptible strains isolated from neonates were included. Biofilm-forming abilities of S. haemolyticus isolates in the presence of sub-optimal CTX (30 μg/mL) were determined by crystal violet assays and extracellular DNA (eDNA) quantitation. CTX was found to significantly enhance biofilm production among the non-susceptible isolates (p-valueWilcoxintest—0.000008) with an increase in eDNA levels (p-valueWilcoxintest—0.000004). Further, in the absence of antibiotic selection in vitro, populations of MDR isolates, JNM56C1 and JNM60C2 remained antibiotic non-susceptible after >500 generations of growth. These findings demonstrate that sub-optimal concentration of CTX induces biofilm formation and short-term non-exposure to antibiotics does not alter non-susceptibility among S. haemolyticus isolates under the tested conditions.

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Effect of Black Grape Seed Extract (Vitis vinifera) on Biofilm Formation of Methicillin-Resistant Staphylococcus aureus and Staphylococcus haemolyticus

Cited by 19 publications

References 32 publications

Biological activities of Egyptian grape and mulberry by-products and their potential use as natural sources of food additives and nutraceuticals foods

Biological activities of Egyptian grape and mulberry by-products and their potential use as natural sources of food additives and nutraceuticals foods

Incorporation of grape seed extract towards wound care product development

Influence of Sub-Inhibitory Dosage of Cefotaxime on Multidrug Resistant Staphylococcus haemolyticus Isolated from Sick Neonatal Care Unit

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