Effect of Biphasic Electrical Current Stimulation on IL-1β–Stimulated Annulus Fibrosus Cells Using In Vitro Microcurrent Generating Chamber System

Kim, Joo Han; Choi, Hyuk; Suh, Min Ji; Shin, Jae Hee; Hwang, Myung Jin; Lee, Heung Man

doi:10.1097/brs.0b013e3182a211e3

Cited by 32 publications

(30 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the precise mechanism is still unknown. The reduction of IL-1β by the microcurrent therapy may have lead to the possible involvement of nuclear factor κB (NFκB) and mitogen-activated protein kinase (MAPK) 27 .…”

Section: Discussionmentioning

confidence: 99%

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Korelo¹,

Kryczyk²,

García³

et al. 2016

Braz. J. Phys. Ther.

View full text Add to dashboard Cite

BACKGROUND: Therapeutic high-frequency ultrasound, microcurrent, and a combination of the two have been used as potential interventions in the soft tissue healing process, but little is known about their effect on the immune system. OBJECTIVE: To evaluate the effects of therapeutic high frequency ultrasound, microcurrent, and the combined therapy of the two on the size of the wound area, peritoneal macrophage function, CD4+ and CD8+, T lymphocyte populations, and plasma concentration of interleukins (ILs). METHOD: Sixty-five Wistar rats were randomized into five groups, as follows: uninjured control (C, group 1), lesion and no treatment (L, group 2), lesion treated with ultrasound (LU, group 3), lesion treated with microcurrent (LM, group 4), and lesion treated with combined therapy (LUM, group 5). For groups 3, 4 and 5, treatment was initiated 24 hours after surgery under anesthesia and each group was allocated into three different subgroups (n=5) to allow for the use of the different therapy resources at on days 3, 7 and 14 Photoplanimetry was performed daily. After euthanasia, blood was collected for immune analysis. RESULTS: Ultrasound increased the phagocytic capacity and the production of nitric oxide by macrophages and induced the reduction of CD4+ cells, the CD4+/CD8+ ratio, and the plasma concentration of IL-1β. Microcurrent and combined therapy decreased the production of superoxide anion, nitric oxide, CD4+-positive cells, the CD4+/CD8+ ratio, and IL-1β concentration. CONCLUSIONS:Therapeutic high-frequency ultrasound, microcurrent, and combined therapy changed the activity of the innate and adaptive immune system during healing process but did not accelerate the closure of the wound.

show abstract

Section: Discussionmentioning

confidence: 99%

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Korelo¹,

Kryczyk²,

García³

et al. 2016

Braz. J. Phys. Ther.

View full text Add to dashboard Cite

show abstract

“…For the evaluation of the response to inflammation, 10 −8 M 1,25(OH) 2 D 3, or vehicle (0.1% DMSO), was added to 80% confluent cells in 5% FBS in the presence or absence of 1 ng/mL IL-1β (Sigma-Aldrich). The cells and supernatant were then evaluated after 48 h of treatment [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Vitamin D’s Effect on the Proliferation and Inflammation of Human Intervertebral Disc Cells in Relation to the Functional Vitamin D Receptor Gene FokI Polymorphism

Luca

Girolamo

Orfei

et al. 2018

IJMS

View full text Add to dashboard Cite

Vitamin D is known to have immunomodulatory effects, is involved in osteo-cartilaginous metabolism, and may have a role in human intervertebral disc pathophysiology. Although a link between vitamin D receptor (VDR) gene variants and disc degeneration-related pathologies has been observed, its functional contribution to pathologic processes has not been assessed yet. The aim of this study was to investigate the response of disc cells to vitamin D in terms of the regulation of proliferation, metabolism, and inflammatory processes, with a particular focus on the FokI VDR genotype. However, although it was found that vitamin D had a pro-apoptotic effect regardless of genotype, an up-regulation of IL-1Ra and downregulation of IL-6 was found to be evident only in Ff cells. Regarding the metabolic effects, in Ff cells, vitamin D promoted an upregulation of the aggrecan in inflammatory conditions but did not have an effect on the expression of collagen-related markers. Moreover, cells bearing the Ff genotype were the most responsive to vitamin D in the upregulation of catabolic markers. In addition, in contrast to the FF genotype, vitamin D downregulated the vitamin D-dependent signaling pathway in inflamed Ff cells, counteracting the inflammation-mediated catabolic effects. In conclusion, Ff cells were found to be more responsive to the anti-inflammatory and catabolic effects of vitamin D, which is likely to be related to matrix remodeling.

show abstract

“…Petrofsky et al [27] showed that an EF of a biphasic sine wave stimulating the quadriceps muscle played a role in the NO-mediated blood flood response to electrical stimulation. Kim et al [28] found that a biphasic electric current (250 and 500 mV/mm) stimulated several factors including NO production in annulus fibrosus cells. Previous studies have shown that VEGF induces an increase in NO production from endothelial cells [12,13] and that inhibition of PI3K activity using PI3K inhibitors attenuates NO release from the cells; VEGF stimulates Aktmediated eNOS phosphorylation, resulting in an increase in eNOS activity [29].…”

Section: Discussionmentioning

confidence: 99%

Direct Current Electric Field Stimulates Nitric Oxide Production and Promotes NO-Dependent Angiogenesis: Involvement of the PI3K/Akt Signaling Pathway

et al. 2020

View full text Add to dashboard Cite

Electric fields (EFs) promote angiogenesis in vitro and in vivo. These results indicate the feasibility of the application of EFs to modulate angiogenesis. Nitric oxide (NO) derived from endothelial nitric oxide synthase (eNOS) is an important regulator of angiogenesis. However, the role of direct current EFs in eNOS activity and expression in association with angiogenesis of endothelial cells has not been investigated. In the present study, we stimulated human umbilical vein endothelial cells (HUVECs) with EFs and evaluated the activity and expression of eNOS. EFs induced significant phosphorylation of eNOS, upregulation of the expression of eNOS protein, and an increase in NO production from HUVECs. L-NAME, a specific inhibitor of eNOS, abolished EF-induced HUVEC angiogenesis. EFs stimulated Akt activation. Inhibition of PI3K activity inhibited EF-mediated Akt and eNOS activation and inhibited NO production in the endothelial cells. Moreover, EFs stimulated HUVEC proliferation and enhanced the S phase cell population of the cell cycle. We conclude that EFs stimulate eNOS activation and NO production via a PI3K/Akt-dependent pathway. Thus, activation of eNOS appears to be one of the key signaling pathways necessary for EF-mediated angiogenesis. These novel findings suggest that NO signaling may have an important role in EF-mediated endothelial cell function.

show abstract

Effect of Biphasic Electrical Current Stimulation on IL-1β–Stimulated Annulus Fibrosus Cells Using In Vitro Microcurrent Generating Chamber System

Abstract: N/A.

Cited by 32 publications

References 38 publications

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Vitamin D’s Effect on the Proliferation and Inflammation of Human Intervertebral Disc Cells in Relation to the Functional Vitamin D Receptor Gene FokI Polymorphism

Direct Current Electric Field Stimulates Nitric Oxide Production and Promotes NO-Dependent Angiogenesis: Involvement of the PI3K/Akt Signaling Pathway

Contact Info

Product

Resources

About